食品与发酵工业 >

2022 , Vol. 48 >Issue 16: 182 - 187

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030252

研究报告

紫山药多酚分离纯化及其对α-葡萄糖苷酶活性的抑制作用

  • 朱延胜 ,
  • 魏明 ,
  • 钱森和 ,
  • 赵世光
展开
  • (安徽工程大学 生物与食品工程学院,安徽 芜湖,241000)
硕士研究生(魏明教授为通信作者,E-mail:wmrainbow69@126.com)

收稿日期: 2021-12-15

  修回日期: 2021-12-30

  网络出版日期: 2022-09-16

基金资助

安徽省高校自然科学基金(KJ2020A0374)

收起

Purification and α-glucosidase inhibitory activity of polyphenols from purple yam

  • ZHU Yansheng ,
  • WEI Ming ,
  • QIAN Senhe ,
  • ZHAO Shiguang
Expand
  • College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China

Received date: 2021-12-15

  Revised date: 2021-12-30

  Online published: 2022-09-16

Fold

摘要

为了分离纯化紫山药中的多酚,研究了5种大孔树脂对紫山药多酚的吸附和解吸特性,筛选出了较适宜的树脂,并对其纯化工艺进行了优化。采用不同溶剂进行洗脱,评价不同洗脱组分对α-葡萄糖苷酶活性的抑制效果,分析了不同洗脱组分的单酚组成。结果表明,AB-8大孔树脂是较适于紫山药多酚分离纯化的吸附介质,其最佳纯化条件为:粗多酚溶液pH为5.0,质量浓度为2.0 g/L,上样流速为1.0 mL/min,吸附平衡后,用50%的乙醇进行洗脱,洗脱流速为1.2 mL/min时,纯化效果较好,经大孔树脂纯化后,多酚纯度为59.06%,提升了3.25倍。比较了50%乙醇、乙酸乙酯和三氯甲烷洗脱组分的α-葡萄糖苷酶抑制活性,其中乙酸乙酯洗脱组分对α-葡萄糖苷酶活性抑制效果最好,其IC50值为0.126 g/L;经高效液相色谱分析可知,其主要含有芦丁、阿魏酸、表儿茶素、绿原酸和槲皮素,说明紫山药多酚具有较高的开发价值。

关键词： 紫山药; 多酚; 大孔树脂; 纯化; α-葡萄糖苷酶

本文引用格式

朱延胜 , 魏明 , 钱森和 , 赵世光 . 紫山药多酚分离纯化及其对α-葡萄糖苷酶活性的抑制作用[J]. 食品与发酵工业, 2022 , 48(16) : 182 -187 . DOI: 10.13995/j.cnki.11-1802/ts.030252

Abstract

In order to purify crude polyphenols from purple yam, the adsorption-desorption properties of purple yam polyphenols by five macroporous resins were investigated. The suitable resin for separating and purifying purple yam polyphenols was obtained and the purification parameters were optimized. Using different solvents for elution, the inhibitory activity of different elution fractions on α-glucosidase was evaluated and the monophenol constituents were analyzed. The results showed that AB-8 macroporous resin was an ideal adsorbent for separating and purifying purple yam polyphenols. The optimum purification parameters were as follows, crude polyphenol 2.0 g/L, pH 5.0, flow velocity of 1.0 mL/min, and 50% ethanol was used as the eluent solvent with the elution flow rate of 1.2 mL/min. The results indicated that the purity of purple yam polyphenols reached 59.06% after purification by AB-8 resin, which increased by 3.25 times than that of the crude polyphenol extract. Three elution components were obtained by using 50% ethanol, chloroform and ethyl acetate as eluents, respectively. The ethyl acetate elution component had the best inhibitory effect on α-glucosidase activity, which contained chlorogenic acid, ferulic acid, rutin, and quercetin. In conclusion, purple yam polyphenols have good utilization and development prospects.

Key words： purple yam; polyphenols; macroporous resin; purification; α-glucosidase

参考文献

[1] 于东.紫山药功能性营养成分及加工技术研究[D].杭州:浙江大学, 2011.
YU D.Study on the functional nutrients and processing technology of purple yam[D].Hangzhou:Zhejiang University, 2011.
[2] 王彦平, 宿时, 陈月英.紫山药多糖超声结合酶法提取工艺优化及抗氧化活性研究[J].食品工业科技, 2017, 38(8): 189-192;198.
WANG Y P, SU S, CHEN Y Y.Study on optimization of ultrasonic-enzymatic extraction of polysaccharides from purple yam and its antioxidant activity[J].Science and Technology of Food Industry, 2017, 38(8):189-192;198.
[3] MORIYA C, HOSOYA T, AGAWA S, et al.New acylated anthocyanins from purple yam and their antioxidant activity[J].Bioscience, Biotechnology, and Biochemistry, 2015, 79(9):1 484-1 492.
[4] CHRISTINA Y I, RIFA′I M. Bioactivity of purple yam tuber (Dioscorea alata L.) on the level of CD8+and CD8+CD462L+ T cells and histology of liver in BALB/c mice model of digestive allergy[J]. The Journal of Experimental Life Sciences, 2014, 4(2):27-33.
[5] HE K, SHI J C, MAO X M.Safety and efficacy of acarbose in the treatment of diabetes in Chinese patients[J].Therapeutics and Clinical Risk Management, 2014, 10(1):505-511.
[6] KWON Y I, APOSTOLIDIS E, SHETTY K.In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension[J].Bioresource Technology, 2008, 99(8):2 981-2 988.
[7] LES F, ARBONS-MAINAR J M, VALERO M S, et al.Pomegranate polyphenols and urolithin A inhibit α-glucosidase, dipeptidyl peptidase-4, lipase, triglyceride accumulation and adipogenesis related genes in 3T3-L1 adipocyte-like cells[J].Journal of Ethnopharmacology, 2018, 220:67-74.
[8] GUO X X, SHA X H, LIU J, et al.Chinese purple yam (Dioscorea alata L.) extracts inhibit diabetes-related enzymes and protect HepG2 cells against oxidative stress and insulin resistance induced by FFA[J].Food Science and Technology Research, 2015, 21(5):677-683.
[9] IGNAT I, VOLF I, POPA V I.A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables[J].Food Chemistry, 2011, 126(4):1 821-1 835.
[10] GUO C X, QIAO J P, ZHANG S W, et al.Purification of polyphenols from kiwi fruit peel extracts using macroporous resins and high-performance liquid chromatography analysis[J].International Journal of Food Science & Technology, 2018, 53(6):1 486-1 493.
[11] GAO N X, WANG Y H, JIAO X Y, et al.Preparative purification of polyphenols from Aronia melanocarpa (chokeberry) with cellular antioxidant and antiproliferative activity[J].Molecules(Based,Switzerland), 2018, 23(1):139.
[12] 杨希娟, 党斌, 张杰, 等.黑青稞麸皮结合态酚类物质大孔树脂分离纯化工艺优化[J].农业工程学报, 2018, 34(21):295-303.
YANG X J, DANG B, ZHANG J, et al.Process optimization on separation and purification of bound polyphenol in black highland barley bran by macroporous resin[J].Transactions of the Chinese Society of Agricultural Engineering, 2018, 34(21):295-303.
[13] ZHANG J H, ZONG A Z, XU T C, et al. A novel method: Ionic liquid-based ultrasound-assisted extraction of polyphenols from Chinese purple yam[J]. Natural Product Research, 2018, 32(7):863-866.
[14] 巫永华,张建萍,赵节昌,等.大孔树脂纯化黄精多酚及其抗氧化性与组成分析[J].农业工程学报, 2020,36(1):318-326.
WU Y H, ZHANG J P, ZHAO J C, et al.Antioxidation and composition analysis of purified polygonatum sibiricum Polyphenols using macroporous resin[J].Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(1):318-326.
[15] LUENGO E, LVAREZ I, RASO J.Improving the pressing extraction of polyphenols of orange peel by pulsed electric fields[J].Innovative Food Science & Emerging Technologies, 2013, 17:79-84.
[16] GHANI U, NUR-E-ALAM M, YOUSAF M, et al.Natural flavonoid α-glucosidase inhibitors from Retama raetam:Enzyme inhibition and molecular docking reveal important interactions with the enzyme active site[J].Bioorganic Chemistry, 2019, 87:736-742.
[17] 巫永华, 刘恩岐, 张建萍, 等.山楂叶多酚的纯化及其抗氧化特性与组分分析[J].食品与发酵工业, 2020, 46(2):165-172.
WU Y H, LIU E Q, ZHANG J P, et al.Purification, antioxidant capacity and component analysis of hawthorn leaf polyphenols[J].Food and Fermentation Industries, 2020, 46(2):165-172.
[18] WANG C, XIE Y T, WANG H Y, et al.Phenolic compounds in beer inhibit formation of polycyclic aromatic hydrocarbons from charcoal-grilled chicken wings[J].Food Chemistry, 2019, 294(10):578-586.
[19] SUN L J, GUO Y R, FU C C, et al.Simultaneous separation and purification of total polyphenols, chlorogenic acid and phlorizin from thinned young apples[J].Food Chemistry, 2013, 136(2):1 022-1 029
[20] 甘芝霖, 倪元颖, 郭悦, 等.大孔树脂分离纯化玫瑰果多酚及其抗氧化性[J].农业工程学报, 2015, 31(24):298-306.
GAN Z L, NI Y Y, GUO Y, et al.Separation and purification of rosehip polyphenols by macroporous resin and its antioxidant activity[J].Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(24):298-306.
[21] 马乐, 韩军岐, 白欢欢, 等.采用大孔树脂分离纯化核桃青皮中多酚[J].食品与发酵工业, 2016, 42(3):237-242.
MA L, HAN J Q, BAI H H, et al.Separation and purification of polyphenols from walnut green husk by macroporous resin[J].Food and Fermentation Industries, 2016, 42(3):237-242.
[22] ZHANG L, ZHU M F, TU Z C, et al.α-Glucosidase inhibition, anti-glycation and antioxidant activities of Liquidambar formosana Hance leaf, and identification of phytochemical profile[J].South African Journal of Botany, 2017, 113:239-247.
[23] 曾桥, 韦承伯, 夏飞, 等.响应面法优化超声波辅助提取杜仲叶茯砖茶绿原酸及其体外降血糖抗氧化活性[J].食品与发酵工业, 2018, 44 (9):204-211.
ZENG Q, WEI C B, XIA F, et al.Optimization of ultrasonic-assisted extraction of Eucommia ulmoides leaves Fu brick tea chlorogenic acid via response surface analysis and its hypoglycemic and antioxidant activities in vitro[J].Food and Fermentation Industries, 2018, 44(9):204-211.
[24] IRONDI E A, OBOH G, AKINDAHUNSI A A, et al.Phenolic composition and inhibitory activity of Mangifera indica and Mucuna urens seeds extracts against key enzymes linked to the pathology and complications of type 2 diabetes[J].Asian Pacific Journal of Tropical Biomedicine, 2014, 4(11):903-910.
[25] LI Y Q, ZHOU F C, GAO F, et al.Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of α-glucosidase[J].Journal of Agricultural and Food Chemistry, 2009, 57(24):11 463-11 468.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司