苯丙酮尿症是一种因体内缺乏苯丙氨酸羟化酶而导致苯丙氨酸过度积累的氨基酸代谢病,研究与开发苯丙氨酸限制的低蛋白特殊膳食对这类疾病患者的营养补给具有重要意义。该研究筛选具有苯丙氨酸降解活性的益生菌,并评价其生产低苯丙胺酸发酵乳的潜力。结果表明:植物乳杆菌(Lactobacillus plantarum)YZX21和动物双歧杆菌(Bifidobacterium animalis)F1-7具有降解苯丙氨酸的能力,降解率分别为12.43%和10.09%。菌株的胃肠环境耐受实验显示,L.plantarum YZX21和B.animalis F1-7均具有良好的胃肠道耐受性、自聚集和黏附能力。与B.animali F1-7相比,L.plantarum YZX21具有优良发酵特性,可作为发酵乳生产的辅助发酵菌株。与市售发酵乳相比,添加L.plantarum YZX21使发酵乳中的游离苯丙氨酸含量降低13.10%。此外,添加L.plantarum YZX21发酵乳的感官评价优于商业发酵乳。筛选得到的L.plantarum YZX21具有明显的苯丙氨酸降解活性,而且具有优良的发酵特性与益生特性,为研究与开发针对苯丙酮尿症的特医食品奠定了基础。
马欣莹
,
纪媛媛
,
刘奥
,
周炜
,
印伯星
,
刘同杰
,
公丕民
,
张兰威
,
易华西
. 降解苯丙氨酸益生菌的筛选及其在酸奶发酵中的应用[J]. 食品与发酵工业, 2022
, 48(19)
: 204
-209
.
DOI: 10.13995/j.cnki.11-1802/ts.031031
Phenylketonuria is an amino acid metabolic disorder caused by the deficiency of phenylalanine hydroxylase, which leads to the over-accumulation of phenylalanine in body. The phenylalanine-restricted low-protein diet plays a significant role of nutrition supply to phenylketonuria patients. The aim of this study was to screen the probiotics with phenylalanine-degrading activity and evaluate the potential application of fermented milk production. The results showed that Lactobacillus plantarum YZX21 and Bifidobacterium animalis F1-7 exhibited high phenylalanine degradation ability. The degradation rates were 12.43% and 10.09% respectively. Moreover, L. plantarum YZX21 and B. animalis F1-7 had good gastrointestinal tolerance, self-aggregation and adhesion ability. Compared with B. animalis F1-7, L. plantarum YZX21 showed better fermentation properties and could be used as an auxiliary starter strain to produce fermented milk. As well as compared with the commercially fermented milk, the content of phenylalanine in fermented milk inoculated with L. plantarum YZX21 was decreased by 13.10%. In addition, the sensory characteristics of the fermented milk with L. plantarum YZX21was better than that of commercially fermented milk. These findings indicated that L. plantarum YZX21 had high phenylalanine degradation ability, excellent fermentation and probiotic properties, which laid the foundation for the development of foods for special medical purpose to phenylketonuria patients.
[1] SHINTAKU H, OHURA T, TAKAYANAGI M, et al.Guide for diagnosis and treatment of hyperphenylalaninemia[J].Pediatrics International:Official Journal of the Japan Pediatric Society, 2021, 63(1):8-12.
[2] TREPP R, MURI R, ABGOTTSPON S, et al.Impact of phenylalanine on cognitive, cerebral, and neurometabolic parameters in adult patients with phenylketonuria (the PICO study):A randomized, placebo-controlled, crossover, noninferiority trial[J].Trials, 2020, 21(1):178.
[3] SU Y J, WANG H J, REJIAFU N, et al.The molecular epidemiology of hyperphenylalaninemia in Uygur population:Incidence from newborn screening and mutational spectra[J].Annals of Translational Medicine, 2019, 7(12):258.
[4] BUSHUEVA T V, BOROVIK T E, SEMYONOVA N N, et al.Use of low-protein enriched starch products in diet therapy of children with phenylketonuria aged over one year[J].Pediatric Pharmacology, 2018, 15(2):129-134.
[5] ZHANG Z, GAO J J, FENG Y, et al.Mutational spectrum of the phenylalanine hydroxylase gene in patients with phenylketonuria in the central region of China[J].Scandinavian Journal of Clinical and Laboratory Investigation, 2018, 78(3):211-218.
[6] GRISCH-CHAN H M, SCHWANK G, HARDING C O, et al.State-of-the-art 2019 on gene therapy for phenylketonuria[J].Human Gene Therapy, 2019, 30(10):1 274-1 283.
[7] GOLDSTEIN A, VOCKLEY J.Clinical trials examining treatments for inborn errors of amino acid metabolism[J].Expert Opinion on Orphan Drugs, 2017, 5(2):153-164.
[8] REID G, ABRAHAMSSON T, BAILEY M, et al.How do probiotics and prebiotics function at distant sites?[J].Beneficial Microbes, 2017, 8(4):521-533.
[9] 黄珊. 基于转录组学与代谢组学的Streptococcus thermophilus TF96氨基酸代谢机制研究[D].哈尔滨:东北农业大学, 2017.
HUANG S.Integrating transcriptomics and metabolomics to reveal metabolism of amino acid of Streptococcus thermophilus TF96[D].Harbin:Northeast Agricultural University, 2017.
[10] ISABELLA V M, HA B N, CASTILLO M J, et al.Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria[J].Nature Biotechnology, 2018, 36(9):857-864.
[11] PINTO A, ALMEIDA M F, RAMOS P C, et al.Nutritional status in patients with phenylketonuria using glycomacropeptide as their major protein source[J].European Journal of Clinical Nutrition, 2017, 71(10):1 230-1 234.
[12] SU Y, LI N, LIANG Z Q.Stabilization of phenylalanine ammonia lyase activity in the yeast[J].Chemistry of Natural Compounds, 2007, 43(5):639-640.
[13] 康小红, 王凡, 陈建国, 等.嗜热链球菌MN-ZLW-002体外益生特性与安全性分析[J].食品工业科技, 2020, 41(15):124-128.
KANG X H, WANG F, CHEN J G, et al.The probiotic properties in vitro and safety analysis of Streptococcus thermophiles MN-ZLW-002[J].Science and Technology of Food Industry, 2020, 41(15):124-128.
[14] 丁淑娟. 降胆固醇乳酸菌筛选及其在高脂大鼠模型上的应用评价[D].广州:华南理工大学, 2019.
DING S J.Screening and comparative analysis of cholesterol-lowing Lactobacillus[D].Guangzhou:South China University of Technology, 2019.
[15] SHAHEEN A, EL-BAGHDADY K, ZAKARIA A.Isolation and identification of potent probiotics with high lead removal capability[J].Egyptian Journal of Botany, 2018,59(1):95-105.
[16] 丁诗瑶, 雷文平, 刘成国, 等.不同来源植物乳杆菌的益生特性研究[J].中国乳品工业, 2021, 49(1):20-24.
DING S Y, LEI W P, LIU C G, et al.Probiotic characteristics of Lactobacillus plantarum strains isolated from different habitats[J].China Dairy Industry, 2021, 49(1):20-24.
[17] RAI R, TAMANG J P.In vitro and genetic screening of probiotic properties of lactic acid bacteria isolated from naturally fermented cow-milk and yak-milk products of Sikkim, India[J].World Journal of Microbiology & Biotechnology, 2022, 38(2):25.
[18] 张莉. 植物乳杆菌的黏附特性研究及其在益生菌干酪中的应用[D].长春:吉林大学, 2013.
ZHANG L.Study on adhesion properties of Lactobacillus plantarum and applications in probiotic cheese[D].Changchun:Jilin University, 2013.
[19] YANG S O, KIM S H, CHO S, et al.Classification of fermented soymilk during fermentation by 1H NMR coupled with principal component analysis and elucidation of free-radical scavenging activities[J].Bioscience, Biotechnology, and Biochemistry, 2009, 73(5):1 184-1 188.
