食品与发酵工业 >

2022 , Vol. 48 >Issue 20: 44 - 50

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030736

研究报告

醋糟固态厌氧消化特性及微生物群落结构分析

  • 张周 ,
  • 张雅晶 ,
  • 周云龙 ,
  • 许之扬 ,
  • 施万胜 ,
  • 阮文权
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  • 1(江南大学 环境与土木工程学院,江苏 无锡,214122)
    2(无锡市太湖湖泊治理股份有限公司,江苏 无锡,214062)
    3(江苏晨洁再生资源科技有限公司,江苏 张家港,215600)
硕士,实验师(阮文权教授为通信作者,E-mail:wqruan@jiangnan.edu.cn)

收稿日期: 2022-01-13

  修回日期: 2022-02-14

  网络出版日期: 2022-11-18

基金资助

国家重点研发计划“固废资源化”重点项目(2018YFC1903000);国家重点研发计划绿色生物制造专项(2021YFC2102203)

收起

Solid-state anaerobic digestion characteristics of vinegar residue and microbial community structure analysis

  • 张周 ,
  • 张雅晶 ,
  • 周云龙 ,
  • 许之扬 ,
  • 施万胜 ,
  • 阮文权
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  • 1(School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China)
    2(Wuxi Taihu Lake Restoration Co. Ltd., Wuxi 214062, China)
    3(Jiangsu Chenjie Renewable Resources Technology Co. Ltd., Zhangjiagang 215600, China)

Received date: 2022-01-13

  Revised date: 2022-02-14

  Online published: 2022-11-18

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摘要

醋糟产生量巨大,对其进行有效处理十分必要,厌氧消化是实现醋糟资源化、减量化的重要途径。采用全混式反应器构建高固态连续式厌氧消化体系对醋糟进行厌氧发酵处理,研究不同有机负荷(organic loading rate, OLR)条件下,醋糟厌氧消化产气性能、中间代谢产物变化情况及微生物群落结构演替规律。结果表明,在OLR为6.06 g/(L·d)条件下表现出最佳的消化性能,沼气产量和甲烷产率分别达423 mL/g和226 mL/g,细菌门水平上拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)相对丰度分别为45.6%和53.1%,4种与木质纤维素代谢有关的菌属相对丰度分布均匀,有利于形成各种代谢途径促进底物高效水解。古菌解析检测到甲烷杆菌属(Methanobacterium)和甲烷八叠球菌属(Methanosarcina)构成主要优势菌属,氢营养型和混合营养型产甲烷途径协同作用。系统稳定运行时总氨氮(total ammonia nitrogen, TAN)和挥发性脂肪酸(volatile fatty acids, VFAs)质量浓度分别维持在2 g/L和9 g/L左右,过高的VFAs浓度会使产甲烷菌活性受到抑制,系统甲烷产率降低。因此,全混式高固态厌氧消化体系能够对醋糟进行高效处理,系统微生物群落丰富度和多样性较高,各类型水解产物均能被体系中多样的产甲烷途径利用。

关键词： 醋糟; 厌氧消化; 有机负荷; 产气性能; 微生物群落

本文引用格式

张周 , 张雅晶 , 周云龙 , 许之扬 , 施万胜 , 阮文权 . 醋糟固态厌氧消化特性及微生物群落结构分析[J]. 食品与发酵工业, 2022 , 48(20) : 44 -50 . DOI: 10.13995/j.cnki.11-1802/ts.030736

Abstract

As the generation of vinegar residue is increasing, its appropriate disposal is important. Anaerobic digestion is an important way to realize the resourcization and reduction of vinegar residue. A high solid-state continuous anaerobic digestion system was constructed for the vinegar residue treatment by using a full-mixed anaerobic reactor, the biogas generation performance, changes of intermediate metabolites and microbial community structure were investigated under different organic loading rate (OLR). The results indicated that the optimum anaerobic digestion performance was obtained when the organic loading rate reached 6.06 g/(L·d), with the biogas and methane yield of 423 and 226 mL/g, respectively. The relative abundances of Bacteroidetes and Firmicutes were 45.6% and 53.1%, respectively. And the relative abundance distribution of 4 species of microorganism related to lignocellulose metabolism was evenly distributed, which was helpful to the formation of various metabolic pathways to promote substrate hydrolysis. The analysis of archaea showed that Methanobacterium and Methanosarcina were the dominant microorganism, hydrotrophic and mixed-trophic methanogenesis pathways had synergetic effect. When the system was stable, the mass concentrations of total ammonia nitrogen (TAN) and volatile fatty acids (VFAs) were maintained at about 2 and 9 g/L, respectively. High VFAs concentration could inhibit the activity of methanogens and reduce the methane generation rate. Therefore, the fully-mixed high-solid anaerobic digestion system could efficiently treat the vinegar residue. The diversity of microbial community was high, different hydrolysates could be all utilized by methane production pathways in the system.

Key words： vinegar residue; anaerobic digestion; organic loading rate; biogas generation performance; microbial community

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