食品与发酵工业 >

2022 , Vol. 48 >Issue 20: 105 - 111

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029481

研究报告

羧甲基多孔淀粉表征及其对槲皮素吸附研究

  • 王宇霞 ,
  • 马云翔 ,
  • 苟丽娜 ,
  • 李敏 ,
  • 张盛贵
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  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
硕士研究生(张盛贵教授为通信作者,E-mail:zhangshenggui@gsau.edu.cn)

收稿日期: 2021-09-22

  修回日期: 2021-10-11

  网络出版日期: 2022-11-18

基金资助

甘肃省重点研发项目(20YF8NA33);中央引导地方科技发展专项(ZCYD-2020-3)

收起

The characterization of carboxymethyl porous starch and its adsorption on quercetin

  • 王宇霞 ,
  • 马云翔 ,
  • 苟丽娜 ,
  • 李敏 ,
  • 张盛贵
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2021-09-22

  Revised date: 2021-10-11

  Online published: 2022-11-18

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摘要

以马铃薯多孔淀粉(porous starch,PS)为材料,制备具有较好溶解性和吸附性的羧甲基多孔淀粉(carboxymethyl porous starch,CMPS),并应用于生物活性物质载体。实验通过N2-吸/脱附、扫描电镜、液态核磁、吸附性能分析等方法,研究了制备样品CMPS的特性。结果表明,以PS为主体,成功制备了取代度为0.25~1.44的CMPS。PS的比表面积(42.88 m2/g)最大,羧甲基化后CMPS的比表面积(12.06 m2/g)减小,但较羧甲基淀粉(carboxymethyl starch,CMS)的比表面积(0.87 m2/g)明显增加。多孔和羧甲基处理均使淀粉结晶消失,醚化反应降低了淀粉的热稳定性。CMPS吸附槲皮素(carboxymethyl porous starch@quercetin,CMPS@Q)的负载率为13.71%,较CMS吸附槲皮素(carboxymethyl starch@quercetin,CMS@Q)负载率(0.57 %)提高了95.84%。与不溶于水的槲皮素对比,CMPS@Q的水溶性(34.00%)较CMS@Q水溶性(23.33%)提高了45.74%。制备的CMPS具备良好的多孔结构、比表面积、溶解性和吸附性能,可作为低水溶性功能性物质载体,具有良好的应用前景。

关键词： 多孔淀粉; 羧甲基淀粉; 比表面积; 结构表征; 槲皮素

本文引用格式

王宇霞 , 马云翔 , 苟丽娜 , 李敏 , 张盛贵 . 羧甲基多孔淀粉表征及其对槲皮素吸附研究[J]. 食品与发酵工业, 2022 , 48(20) : 105 -111 . DOI: 10.13995/j.cnki.11-1802/ts.029481

Abstract

Potato porous starch (PS) is used as a material to prepare carboxymethyl porous starch (CMPS) with better solubility and adsorption, then CMPS will be used as a carrier of biologically active substances. The experiment used N2-adsorption/desorption, scanning electron microscopy (SEM), liquid nuclear magnetic (1H NMR), adsorption performance analysis and other methods to study the characteristics of the prepared samples CMPS. The results showed that carboxymethyl porous starch (CMPS) with a degree of substitution (DS) of 0.25-1.44 was successfully prepared. The specific surface area of PS (42.88 m2/g) was the largest. After carboxymethylation, the specific surface area (12.06 m2/g) of CMPS decreased. However, compared with the specific surface area (0.87 m2/g) of carboxymethyl starch (CMS), it was still significantly increased. Both porous and carboxymethyl treatments made the starch crystal disappear, and the etherification reaction reduced the thermal stability of the starch. The loading rate of CMPS@Q was 13.71%, which was 95.84% higher than the loading rate of CMS@Q (0.57%). Compared with quercetin insoluble in water, the water solubility of CMPS@Q (34.00%) was 45.74% higher than that of CMS@Q (23.33%). The prepared CMPS has a good porous structure, specific surface area, solubility and adsorption performance, and can be used as a carrier of low water-soluble functional substances.

Key words： porous starch; carboxymethyl starch; specific surface area; structural characterization; quercetin

参考文献

[1] KAUR B, ARIFFIN F, BHAT R, et al.Progress in starch modification in the last decade[J].Food Hydrocolloids, 2012, 26(2):398-404.
[2] WILPISZEWSKA K, ANTOSIK A K, SPYCHAJ T.Novel hydrophilic carboxymethyl starch/montmorillonite nanocomposite films[J].Carbohydrate Polymers, 2015, 128:82-89.
[3] LIU J, WANG X C, YONG H M, et al.Preparation, characterization, digestibility and antioxidant activity of quercetin grafted Cynanchum auriculatum starch[J].International Journal of Biological Macromolecules, 2018, 114:130-136.
[4] 卢双彦, 张雅楠, 翟光喜.槲皮素纳米制剂研究进展[J].药物生物技术, 2019, 26(6):552-557.
LU S Y, ZHANG Y N, ZHAI G X.Development of the quercetin loaded nanoformulations[J].Pharmaceutical Biotechnology, 2019, 26(6):552-557.
[5] LIN J, TEO L M, LEONG L P, et al.In vitro bioaccessibility and bioavailability of quercetin from the quercetin-fortified bread products with reduced glycemic potential[J].Food Chemistry, 2019, 286:629-635.
[6] CHEN J H, WANG Y X, LIU J, et al.Preparation, characterization, physicochemical property and potential application of porous starch:A review[J].International Journal of Biological Macromolecules, 2020, 148:1 169-1 181.
[7] JU J, CHEN X Q, XIE Y F, et al.Simple microencapsulation of plant essential oil in porous starch granules:Adsorption kinetics and antibacterial activity evaluation[J].Journal of Food Processing and Preservation, 2019, 43(10):e14156.
[8] WANG H L, LYU J, JIANG S W, et al.Preparation and characterization of porous corn starch and its adsorption toward grape seed proanthocyanidins[J].Starch-Stárke, 2016, 68(11-12):1 254-1 263.
[9] HU J, TIAN T, XIAO Z B.Preparation of cross-linked porous starch and its adsorption for chromium (VI) in tannery wastewater[J].Polymers for Advanced Technologies, 2015, 26(10):1 259-1 266.
[10] BUDARIN V, CLARK J H, HARDY J J E, et al.Starbons:New starch-derived mesoporous carbonaceous materials with tunable properties[J].Angewandte Chemie International Edition, 2006, 45(23):3 782-3 786.
[11] ZHANG B, LI X M, XIE Q T, et al.Preparation and characterization of non-crystalline granular starch and corresponding carboxymethyl starch[J].International Journal of Biological Macromolecules, 2017, 103:656-662.
[12] 陈庆. 直链淀粉的羧化和多孔改性及其对氨和苯酚的吸附性能研究[D].杭州:浙江大学, 2017.
CHEN Q.Study on carboxylation, pore-forming of amylose and its ammonia and phenol adsorption properties[D].Hangzhou:Zhejiang University, 2017.
[13] AHMAD M, MUDGIL P, GANI A, et al.Nano-encapsulation of catechin in starch nanoparticles:Characterization, release behavior and bioactivity retention during simulated in-vitro digestion[J].Food Chemistry, 2019, 270:95-104.
[14] 刘维信, 冯希环, 蔡宋宋, 等.大葱槲皮素含量的测定[J].中国农学通报, 2008,24(3):266-269.
LIU W X, FENG X H, CAI S S, et al.Evaluation of quercetin content in Welsh onion[J].Chinese Agricultural Science Bulletin, 2008,24(3):266-269.
[15] 毕英慧. 羧甲基马铃薯淀粉的制备、反应动力学及性能研究[D].兰州:兰州大学, 2009.
BI Y H.Study on the preparation, reaction kinetics and properties of carboxymethyl potato starch[D].Lanzhou:Lanzhou University, 2009.
[16] 李海燕, 马云翔, 俞力月,等.辛烯基琥珀酸酐改性多孔淀粉结构表征[J].中国粮油学报, 2020, 35(6):70-75.
LI H Y, MA Y X, YU L Y, et al.Structure characterizatin of octenyl succinic anhydride modified porous starch[J].Journal of the Chinese Cereals and Oils Association, 2020, 35(6):70-75.
[17] WANG L F, PAN S Y, HU H, et al.Synthesis and properties of carboxymethyl kudzu root starch [J].Carbohydrate Polymers, 2010, 80(1):174-179.
[18] ZHANG B, CUI D P, LIU M Z, et al.Corn porous starch:Preparation, characterization and adsorption property[J].International Journal of Biological Macromolecules, 2012, 50(1):250-256.
[19] 苟丽娜, 马云翔, 王宇霞, 等.高比表面积阿魏酸多孔淀粉酯结构表征及体外消化特性[J].食品与发酵工业, 2021,47(22):76-83.
GOU L N, MA Y X, WANG Y X, et al.Structural characterization and in vitro digestibility of ferulic acid porous starch ester with high specific surface area[J].Food and Fermentation Industries, 2021,47(22):76-83.
[20] LIU Y, LU K Y, HU X T, et al.Structure, properties and potential applications of phytoglycogen and waxy starch subjected to carboxymethylation[J].Carbohydrate Polymers, 2020, 234:115908.
[21] NAMAZI H, FATHI F, DADKHAH A.Hydrophobically modified starch using long-chain fatty acids for preparation of nanosized starch particles[J].Scientia Iranica, 2011, 18(3):439-445.
[22] ZHOU M, SHI L, CHENG F, et al.High-efficient preparation of carboxymethyl starch via ball milling with limited solvent content[J].Starch - Stárke, 2018, 70(5-6):1700250.
[23] HE X, GONG X C, LI W F, et al.Preparation and characterization of amphiphilic composites made with double-modified (etherified and esterified) potato starches[J].Starch-Stárke, 2019, 71(9-10):1900089.
[24] SING, K S W.Reporting physisorption data for gas/solid systems with special reference to the determination of surface area and porosity (Recommendations 1984)[J].Pure and Applied Chemistry, 1985, 57(4), 603-619.
[25] UBEYITOGULLARI A, CIFTCI O N.Generating phytosterol nanoparticles in nanoporous bioaerogels via supercritical carbon dioxide impregnation:Effect of impregnation conditions[J].Journal of Food Engineering, 2017, 207:99-107.
[26] LI X, GAO W Y, HUANG L J, et al.Preparation and physicochemical properties of carboxymethyl Fritillaria ussuriensis Maxim.starches[J].Carbohydrate Polymers, 2010, 80(3):768-773.
[27] SUJKA M, PANKIEWICZ U, KOWALSKI R, et al.Porous starch and its application in drug delivery systems[J].Polymers in Medicine, 2018, 48(1):25-29.
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