食品与发酵工业 >

2022 , Vol. 48 >Issue 22: 82 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030666

研究报告

香芹酚-壳聚糖纳米颗粒的制备及抑菌活性研究

  • 邢承宇 ,
  • 王璐 ,
  • 陈星光 ,
  • 陆健 ,
  • 吴殿辉
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  • 1(粮食发酵与食品生物制造国家工程研究中心(江南大学),江苏 无锡,214122)
    2(工业生物技术教育部重点实验室,生物工程学院(江南大学),江苏 无锡,214122)
    3(江苏省生物活性制品加工工程技术研究中心,江苏 无锡,214122)
第一作者：硕士研究生(陆健教授和吴殿辉副研究员为共同通信作者:E-mail:jlu@jiangnan.edu.cn;wudianhui@jiangnan.edu.cn)

收稿日期: 2021-12-31

  修回日期: 2022-02-10

  网络出版日期: 2022-12-20

基金资助

江苏省重点研发计划(BF2018308);高等学校学科创新引智计划(111计划)项目(111-206);江苏高校优势学科建设工程资助项目

收起

Preparation and antifungal activity of chitosan nanoparticle loaded with carvacrol

  • XING Chengyu ,
  • WANG Lu ,
  • CHEN Xingguang ,
  • LU Jian ,
  • WU Dianhui
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  • 1(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing (Jiangnan University), Wuxi 214122, China)
    2(Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, School of Biotechnology, Wuxi 214122, China)
    3(Jiangsu Engineering Technology Research Center for Bioactive Products Processing, Wuxi 214122, China)

Received date: 2021-12-31

  Revised date: 2022-02-10

  Online published: 2022-12-20

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摘要

禾谷镰刀菌(Fusarium graminearum)及其产生的真菌毒素严重危害着谷物的质量与安全,开发新型的绿色抗菌剂是控制粮食霉变和保障食品安全的重要途径之一。该研究采用乳化交联法制备了香芹酚-壳聚糖纳米颗粒,结果显示,香芹酚的包封率在35%~60%,所得纳米颗粒呈现规则分布的球形,尺寸为40~100 nm。通过对香芹酚和香芹酚-壳聚糖纳米颗粒的加速贮藏考察了其对禾谷镰刀菌的抑菌稳定性。在35 ℃环境下贮藏50 d后,香芹酚和香芹酚-壳聚糖纳米颗粒对禾谷镰刀菌的菌丝抑制率分别从82.2%降至25.1%以及70.5%降至47.7%,表明纳米颗粒的制备提高了香芹酚的抑菌稳定性。扫描电子显微镜结果表明,香芹酚-壳聚糖纳米颗粒可以显著破坏禾谷镰刀菌的细胞膜和细胞壁,从而抑制其生长。该研究为解决粮食霉变和粮食中真菌毒素的污染提供了新的思路。

关键词： 禾谷镰刀菌; 香芹酚; 壳聚糖纳米颗粒; 包封率; 抑菌稳定性

本文引用格式

邢承宇 , 王璐 , 陈星光 , 陆健 , 吴殿辉 . 香芹酚-壳聚糖纳米颗粒的制备及抑菌活性研究[J]. 食品与发酵工业, 2022 , 48(22) : 82 -88 . DOI: 10.13995/j.cnki.11-1802/ts.030666

Abstract

Fusarium graminearum and its mycotoxins seriously endanger the quality and safety of grains. The development of new green antifungal agents is still one of the important ways to control grain mildew and ensure food safety. In this study, carvacrol-loaded chitosan nanoparticles were prepared by emulsion-ionic gelation. The results showed that the retention rate of carvacrol ranged from 35% to 60%, and the resulting nanoparticles displayed regular distribution and spherical shape, the size range of which were 40-100 nm. The stability of antifungal activity of carvacrol and carvacrol-chitosan nanoparticles against F. graminearum was investigated by accelerated storage. After 50 d of storage at 35 ℃, the mycelial inhibition against F. graminearum by carvacrol and carvacrol-chitosan nanoparticles decreased from 82.2% to 25.1% and 70.5% to 47.7%, respectively. It indicated that the preparation of nanoparticles improved the inhibition stability of carvacrol. The results of scanning electron microscopy showed that the carvacrol-loaded chitosan nanoparticles could significantly damage the cell membrane and cell wall of F. graminearum, thereby inhibiting its growth. This study provides a new idea to address the contamination of grain mold and fungal toxins in grain.

Key words： Fusarium graminearum; carvacrol; chitosan nanoparticles; retention rate; antifungal stability

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