为开发兼具抗氧化和血管紧张素转化酶(angiotensin converting enzyme, ACE)抑制活性的富硒牡蛎肽,该研究以富硒牡蛎蛋白为原料优化制备富硒牡蛎肽,并对其硒含量、氨基酸组成、抗氧化活性和ACE抑制活性进行评价。结果表明,富硒牡蛎肽的最佳酶解条件为时间4 h、温度37 ℃、酶底比0.5%、pH 1.0、底物质量浓度7 g/100mL。该条件下制备的富硒牡蛎肽富含与抗氧化和ACE抑制活性相关的疏水性氨基酸和酸性氨基酸,其清除DPPH自由基和ABTS阳离子自由基的EC50值分别为1.365、1.074 mg/mL,并呈现出良好的细胞抗氧化活性(EC50:1.114 μg/mL),对HepG2细胞的氧化损伤具有显著的保护作用,且呈量效关系。此外,富硒牡蛎肽的ACE抑制活性较强,这可能与其良好的抗氧化活性具有内在关联性。富硒牡蛎肽具有良好的抗氧化和降血压活性,该研究结果为牡蛎源富硒肽研究奠定了前期基础,为天然富硒营养健康食品的开发提供了理论依据。
To develop selenium-enriched oyster peptides with antioxidant and angiotensin converting enzyme (ACE)inhibitory activities, selenium-enriched oyster peptides were prepared from selenium-enriched oyster protein, and its selenium content, amino acid composition, antioxidant activity, and ACE inhibitory activities were evaluated. Results showed that the optimal enzymatic conditions were as follows: Hydrolysis time of 4 h,temperature of 37 ℃, enzyme/substrate ratio of 0.5%, pH 1.0, and substrate mass concentration of 7 g/100mL. The selenium-enriched oyster peptidesprepared under these conditions were rich in hydrophobic and acidic amino acids related to antioxidant and ACE inhibitory activities, and the EC50 values for scavenging DPPH radicals and ABTS cation radicals were 1.365 and 1.074 mg/mL, respectively. Selenium-enriched oyster peptides showed good cellular antioxidant activity (EC50: 1.114 μg/mL) and had a significant protective effect on oxidative damage of HepG2 cells in a dose-effect relationship. In addition, selenium-enriched oyster peptides showed strong ACE inhibitory activity, which might be intrinsically related to its good antioxidant activity. Selenium-enriched oyster peptides have good antioxidant and antihypertensive activities, which lay a preliminary foundation for the further study of selenium-enriched peptides from oysters, and provide a theoretical basis for the development of natural selenium-enriched nutritional health food.
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