食品与发酵工业 >

2023 , Vol. 49 >Issue 3: 131 - 138

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031670

研究报告

基于图像计数酿酒酵母的统计分析

  • 李雪松 ,
  • 陈翔宇 ,
  • 李蒙蒙 ,
  • 徐娟 ,
  • 林丽军 ,
  • 陆利霞 ,
  • 崔晓文 ,
  • 熊晓辉
展开
  • (南京工业大学 食品与轻工学院,南京,211800)
硕士研究生(林丽军讲师为通信作者,E-mail:lljnjut@126.com)

收稿日期: 2022-03-23

  修回日期: 2022-05-22

  网络出版日期: 2023-03-03

基金资助

国家重点研发计划项目(2018YFC1602800)

收起

Statistical analysis of Saccharomyces cerevisiae based on image counting

  • LI Xuesong ,
  • CHEN Xiangyu ,
  • LI Mengmeng ,
  • XU Juan ,
  • LIN Lijun ,
  • LU Lixia ,
  • CUI Xiaowen ,
  • XIONG Xiaohui
Expand
  • (College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211800, China)

Received date: 2022-03-23

  Revised date: 2022-05-22

  Online published: 2023-03-03

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摘要

图像计数可快速定量微生物的活细胞浓度,但对其统计参数及定量结果的可靠性研究较少。该研究以酿酒酵母为材料,研究了自行设计的微型计数板图像计数方法中异常数、中位数、平均数、图像间菌体数量的相对标准偏差等统计量与计数图像数量的关系,比较了图像计数和GB 4789.15—2016平板计数定量的相对标准不确定度,以及结果的差异性和相关性等。结果表明,酿酒酵母105~107 CFU/mL时,图像计数可使用中位数、平均数统计活菌数量;105 CFU/mL时需计数50个图像,106~107 CFU/mL时计数13个图像可准确获得酿酒酵母浓度。图像计数的相对标准不确定度(0.022~0.100)小于平板计数法(0.099)。在105~107 CFU/mL时,图像计数与平板计数对酿酒酵母定量的结果一致。

关键词： 酿酒酵母; 图像计数; 平板计数; 统计分析; 不确定度

本文引用格式

李雪松 , 陈翔宇 , 李蒙蒙 , 徐娟 , 林丽军 , 陆利霞 , 崔晓文 , 熊晓辉 . 基于图像计数酿酒酵母的统计分析[J]. 食品与发酵工业, 2023 , 49(3) : 131 -138 . DOI: 10.13995/j.cnki.11-1802/ts.031670

Abstract

Image counting can quickly quantify the activity cell concentration of microorganisms, but there are few studies on the statistical parameters and the reliability of quantitative results. In this study, Saccharomyces cerevisiae was used as the material and a self-made micro-counting board was designed as the counting tool to study the statistics relationship of the image counting result, such as abnormal data, median, average, relative standard deviation of the activity cell number of S. cerevisiae. The relative standard uncertainty, the difference and correlation of image counting and GB 4789.15—2016 plate counting were compared. The results showed that the median and the average could be used to count the viable number of S. cerevisiae at 105-107 CFU/mL concentration. And 50 images were necessary to be counted in 105 CFU/mL and 13 images in 106-107 CFU/mL for accurately quantified the concentration of S. cerevisiae. The relative standard uncertainty of image counting was at 0.022-0.100 and lower than that of the plate counting method (0.099). It was consistent between image counting and plate counting on the quantitative results of S. cerevisiae at 105-107 CFU/mL concentration.

Key words： Saccharomyces cerevisiae; image counting; plate counting; statistical analysis; uncertainty

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