研究报告

不同发酵剂对泡萝卜挥发性风味成分及感官品质的影响

  • 杨洋 ,
  • 杨宇航 ,
  • 王洪伟 ,
  • 索化夷 ,
  • 宇嘉 ,
  • 张玉
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  • 1(西南大学 食品科学学院,重庆,400700)
    2(西南大学,食品科学与工程国家实验教学示范中心,重庆,400700)
本科生(张玉高级实验师为通信作者,E-mail:zhangyu_512@sina.cn)

收稿日期: 2022-02-23

  修回日期: 2022-05-06

  网络出版日期: 2023-03-20

基金资助

重庆市自然科学基金面上项目(cstc2020jcyj-msxmX201);重庆市北碚区技术创新与应用示范类项目(2020-31)

Effects of different starter on volatile flavor components and sensory properties of pickled radish

  • YANG Yang ,
  • YANG Yuhang ,
  • WANG Hongwei ,
  • SUO Huayi ,
  • YU Jia ,
  • ZHANG Yu
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  • 1(College of Food Science, Southwest University, Chongqing 400700, China)
    2(National Teaching Demonstration Center of Food Science and Engineering, Southwest University, Chongqing 400700, China)

Received date: 2022-02-23

  Revised date: 2022-05-06

  Online published: 2023-03-20

摘要

该文以白萝卜为原料发酵泡萝卜,探究了6种发酵剂对泡菜的挥发性风味成分和感官品质的影响。对不同发酵剂发酵的泡萝卜的挥发性成分进行定性和半定量分析,利用聚类分析法探究挥发性成分与菌种相关性,采用闪现剖面(flash profile,FP)法比较腌制萝卜的品质差异。研究发现,不同发酵剂发酵的泡萝卜共检出75种挥发性成分,包括酯类12种、醇类27种、萜烯类5种、酸类10种、醛酮类4种、烷烃类4种和其他13种,不同的发酵剂发酵泡萝卜所含各类组分的含量和占比均不同。挥发性成分与菌种相关性分析结果表明6种发酵剂菌种与挥发性成分之间具有较大的差异,且真菌和细菌产生的挥发性成分的物质组成也具有较大差异。植物乳杆菌、耐酸乳杆菌和沙雷氏菌是发酵过程中主要细菌组成,毕赤酵母是发酵过程中的主要真菌组成,与醇类、酯类、酸类和萜烯类化合物显著正相关(P<0.01)。感官评价结果表明不同发酵剂发酵的泡萝卜的挥发性成分可被明显区分,感官分析结果与挥发性成分测定结果重合度较高。综合实验结果,HS发酵剂发酵的泡萝卜挥发性风味成分表现较优,并且该发酵剂发酵的泡萝卜感官品质较好。

本文引用格式

杨洋 , 杨宇航 , 王洪伟 , 索化夷 , 宇嘉 , 张玉 . 不同发酵剂对泡萝卜挥发性风味成分及感官品质的影响[J]. 食品与发酵工业, 2023 , 49(4) : 175 -182 . DOI: 10.13995/j.cnki.11-1802/ts.031253

Abstract

The effects of six kinds of starter cultures on the volatile components and sensory quality of pickled white radish were studied. The volatile components of pickled radish were qualitatively and semi-quantitatively analyzed. Cluster analysis was used to explore the correlation between volatile components and strains. Flash Profile(FP) method was used to compare the quality differences of pickled radish. Seventy-five volatile components were detected in pickled radish, including 12 esters, 27 alcohols, 5 terpenes, 10 acids, 4 aldehydes and ketones, 4 alkanes and 13 others. The contents and proportions of components in different pickled radish were different. Significant differences were observed between strains of different starter cultures and volatile components based on correlation analysis, and the composition of volatile components produced by fungi and bacteria was also quite different. Lactobacillus plantarum, Lactobacillus acetotolerans and Serratia were the main bacteria during fermentation, while Pichia pastoris was the main fungi, which was significantly positively correlated with alcohols, esters, acids and terpenes (P<0.01). Sensory evaluation showed that the volatile components of pickled radish fermented by different starter cultures can be clearly distinguished, and the results of sensory analysis were in high coincidence with that of volatile components determination. Based on the results, the HS starter culture performed better in terms of volatile components, and the pickled radish fermented by HS starter cultures had better sensory quality.

参考文献

[1] 陈功. 试论中国泡菜历史与发展[J].食品与发酵科技, 2010, 46(3):1-5.
CHEN G.The history and development of Chinese pickles[J].Food and Fermentation Technology, 2010,46(3):1-5
[2] 王艳, 胡跃, 方红美, 等.白萝卜泡菜加工工艺的研究[J].食品工业科技, 2021, 42(11):185-191.
WANG Y, HU Y, FANG H M, et al.Study on processing technology of pickled radish[J].Science and Technology of Food Industry, 2021, 42(11):185-191.
[3] MARCO M L, HEENEY D, BINDA S, et al.Health benefits of fermented foods:Microbiota and beyond[J].Current Opinion in Biotechnology, 2017,44:94-102.
[4] MARKOWIAK P, ŚLIZ′EWSKA K.Effects of probiotics, prebiotics, and synbiotics on human health[J].Nutrients, 2017, 9(9):1 021.
[5] 陈地. 四川:泡菜年产值超330亿元[J].中国食品, 2018(23):45-46.
CHEN D.Sichuan:The annual output value of pickles exceeds 33 billion yuan[J].China Food, 2018 (23):45-46.
[6] 廖俸, 周淼葭.产业集群 东坡泡菜撑起百亿大市场[J].当代县域经济, 2021(3):28-31.
LIAO F, ZHOU M J.Industrial cluster Dongpo pickles support 10 billion market[J].Contemporary County Economy, 2021(3):28-31.
[7] 周佳, 龙思伊, 仝静雯, 等.乳酸菌接种发酵泡菜工艺研究[J].中国果菜, 2018, 38(12):1-6.
ZHOU J, LONG S Y, TONG J W, et al.Fermentation process of pickles inoculated with lactic acid bacteria[J].China Fruit & Vegetable, 2018, 38(12):1-6.
[8] 刘继业. 直投式乳酸菌发酵剂制备技术的研究及其应用[D].泰安:山东农业大学, 2017.
LIU J Y.Study on preparing technology of the lactic acid bacteria direct vat set and its application[D].Tai’an:Shandong Agricultural University, 2017.
[9] 经骐源, 李婷, 曾凡坤, 等.发酵剂对泡萝卜品质的影响[J].食品科学, 2021, 42(22):171-177.
JING Q Y, LI T, ZENG F K, et al.Effect of starters on the quality of pickled radish[J].Food Science, 2021, 42(22):171-177.
[10] 云琳. 不同发酵方式的萝卜泡菜风味特征解析及发酵剂菌种的筛选[D].无锡:江南大学, 2020.
YUN L.Analysis of the flavor characteristics of radish pickles with different fermentation methods and selection of the starter cultures[D].Wuxi:Jiangnan University, 2020.
[11] 朱海霞. 乳酸菌发酵辣椒泡菜的研究及其发酵剂的制备[D].天津:天津科技大学, 2014.
ZHU H X.Study on pepper pickles fermentation by lactic acid bacteria and its starter culture prepartion[D].Tianjin:Tianjin University of Science and Technology, 2014.
[12] 康娇, 张亚蓉, 杨从绪, 等.FYa1泡菜用直投式发酵剂的制作工艺优化[J].食品科学, 2020, 41(24):216-222.
KANG J, ZHANG Y R, YANG C X, et al.Optimization of the production process for direct vat set of Lactobacillus fermentum FYa1 for pickle fermentation[J].Food Science, 2020, 41(24):216-222.
[13] YANG Y L, FAN Y, LI T, et al.Microbial composition and correlation between microbiota and quality-related physiochemical characteristics in chongqing radish paocai[J].Food Chemistry, 2022, 369:130897.
[14] 汪冬冬, 唐垚, 陈功, 等.不同发酵方式盐渍萝卜挥发性成分动态分析[J].食品科学, 2020, 41(6):146-154.
WANG D D, TANG Y, CHEN G, et al.Dynamic analysis of volatile components of salted radish during different fermentation processes[J].Food Science, 2020, 41(6):146-154.
[15] 姚月华, 索化夷, 赵欣, 等.Flash Profile法和定量描述分析法对市售酸奶和巴氏杀菌酸奶感官性质的分析[J].食品与发酵工业, 2018, 44(12):237-244.
YAO Y H, SUO H Y, ZHAO X, et al.Sensory attributes of commercial yogurts and pasteurized yogurts using Flash Profile and quantitative descriptive analysis[J].Food and Fermentation Industries, 2018, 44(12):237-244.
[16] 李雨萌, 曾里, 吴正云.老坛水发酵与接种发酵过程中细菌菌落与挥发性成分差异研究[J].中国调味品, 2020, 45(12):73-77;86.
LI Y M, ZENG L, WU Z Y.Study on the differences of bacterial colony and volatile components in laotan water fermentation and inoculation fermentation process[J].China Condiment, 2020, 45(12):73-77;86.
[17] 王芮东, 赵燕飞, 邢颖, 等.萝卜泡菜自然发酵过程中挥发性香气成分变化分析[J].中国酿造, 2019, 38(9):124-129.
WANG R D, ZHAO Y F, XING Y, et al.Change of aroma components in natural fermentation of radish kimchi[J].China Brewing, 2019, 38(9):124-129.
[18] YUN L, MAO B Y, CUI S M, et al.Gas chromatography-mass spectrometry-based metabolomics analysis of metabolites in commercial and inoculated pickles[J].Journal of the Science of Food and Agriculture, 2021, 101(4):1 436-1 446.
[19] 张华芳, 车幸轩, 刘阳, 等.芫根泡菜发酵过程中风味物质变化研究[J].中国调味品, 2019, 44(7):65-70.
ZHANG H F, CHE X X, LIU Y, et al.Study on the changes of flavor substances in the fermentation process of Brassica rapa[J].China Condiment, 2019, 44(7):65-70.
[20] 夏季,方勇,王梦梦, 等.不同发酵处理对香菇泡菜质构及风味物质的影响[J].食品科学, 2019, 40(20):171-177.
XIA J, FANG Y, WANG M M, et al.Effects of different fermentation treatments on the texture and flavor compounds of Lentinus edodes kimchi[J].Food Science, 2019, 40(20):171-177.
[21] 郑文迪,关倩倩,刘长根, 等.基于GC-MS 法对比广西地区酸菜和酸笋风味的差异[J].食品与发酵工业, 2020, 46(4):253-257.
ZHENG W D, GUAN Q Q, LIU C G, et al.Comparison of flavors of Suancai and Suansun in Guangxi based on GC-MS[J].Food and Fermentation Industries, 2020, 46(4):253-257.
[22] 徐丹萍, 蒲彪, 陈安均, 等.传统四川泡菜中挥发性成分分析[J].食品与发酵工业, 2014, 40(11):227-232.
XU D P, PU B, CHEN A J, et al.Analysis of volatile components in traditional Sichuan Paocai[J].Food and Fermentation Industries, 2014, 40(11):227-232.
[23] 李庆羊, 吴祖芳, 翁佩芳, 等.植物乳杆菌和棒状乳杆菌对发酵萝卜干风味品质的影响[J].中国食品学报, 2021, 21(9):150-159.
LI Q Y, WU Z F, WENG P F, et al.Effects of Lactobacillus plantarum and Lactobacillus rodarum on flavor quality of fermented dried radish[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(9):150-159.
[24] 陈大鹏, 郑娅, 周芸, 等.自然发酵与人工接种发酵法发酵泡菜的品质比较研究[J].食品工业科技, 2019, 40(18):368-372.
CHEN D P, ZHENG Y, ZHOU Y, et al.Quality comparison of pickled cabbage fermented by natural fermentation and artificial inoculation[J].Science and Technology of Food Industry, 2019, 40(18):368-372.
[25] HU Y Y, ZHANG L, LIU Q, et al.The potential correlation between bacterial diversity and the characteristic volatile flavour of traditional dry sausages from Northeast China[J].Food Microbiology, 2020, 91:103505.
[26] YANG X Z, HU W Z, XIU Z L, et al.Microbial community dynamics and metabolome changes during spontaneous fermentation of Northeast Sauerkraut from different households[J].Frontiers in Microbiology, 2020, 11:1 878.
[27] SHANG Z X, YE Z, LI M Q, et al.Dynamics of microbial communities, flavor, and physicochemical properties of pickled chayote during an industrial-scale natural fermentation:Correlation between microorganisms and metabolites[J].Food Chemistry, 2022, 337:132004.
[28] RAO Y, TAO Y F, CHEN X, et al.The characteristics and correlation of the microbial communities and flavors in traditionally pickled radishes[J].LWT-Food Science and Technology, 2020, 118:108804.
[29] 张慧敏, 姜林君, 赵江欣, 等.预添加乳酸对直投式发酵泡萝卜感官品质的影响[J].基因组学与应用生物学, 2020, 39(1):246-253.
ZHANG H M, JIANG L J, ZHAO J X, et al.The effect on radish pickles direct fermentation process by pre-adding a few lactic acids[J].Genomics and Applied Biology, 2020, 39(1):246-253.
[30] 赵江欣. 预添加乳酸对泡萝卜品质影响的研究[D].雅安:四川农业大学, 2018.
ZHAO J X.Research on the effects of addition of lactic acid before fermentation on the sensory quality of radish pickles[D].Ya’an:Sichuan Agricultural University, 2018.
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