食品与发酵工业 >

2023 , Vol. 49 >Issue 4: 288 - 294

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032056

综述与专题评论

基于分子生物学原理技术检测肉制品中动物源性成分研究进展

  • 易艳 ,
  • 刘明东 ,
  • 蒋子敬 ,
  • 姜展樾 ,
  • 马丽侠 ,
  • 叶德萍 ,
  • 韩国全 ,
  • 周李华
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  • 1(四川农业大学 食品学院,四川 雅安,625000)
    2(中国测试技术研究院,四川 成都,610000)
硕士研究生(周李华研究员和韩国全副教授为共同通信作者,E-mail:375697868@qq.com;hans_980306@sicau.edu.cn)

收稿日期: 2022-04-27

  修回日期: 2022-05-09

  网络出版日期: 2023-03-20

基金资助

国家重点研发计划(2021YFF0701905)

收起

Advance in the detection of animal-derived ingredients in meat products using techniques based on molecular biology principle

  • YI Yan ,
  • LIU Mingdong ,
  • JIANG Zijing ,
  • JIANG Zhanyue ,
  • MA Lixia ,
  • YE Deping ,
  • HAN Guoquan ,
  • ZHOU Lihua
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  • 1(Sichuan Agricultural University, College of Food Science, Ya′an 625000, China)
    2(National Institute of Measurement and Testing Technology, Chengdu 610000, China)

Received date: 2022-04-27

  Revised date: 2022-05-09

  Online published: 2023-03-20

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摘要

肉制品掺假严重侵害消费者对食品质量安全的信任,影响食品行业的健康发展,已成为全球关注的焦点之一。该文综述当前应用较为广泛的基于分子生物学原理的检测技术的优缺点和应用场景,包括普通PCR定性检测、实时荧光PCR相对定量、数字PCR绝对定量、环介导等温扩增快速检测、DNA条形码、高通量测序等新型分子检测技术,以期为政府监管部门及第三方检测机构的研究者提供参考。

关键词： 肉制品掺假; 动物源性成分; 分子生物学技术; 高通量测序; PCR

本文引用格式

易艳 , 刘明东 , 蒋子敬 , 姜展樾 , 马丽侠 , 叶德萍 , 韩国全 , 周李华 . 基于分子生物学原理技术检测肉制品中动物源性成分研究进展[J]. 食品与发酵工业, 2023 , 49(4) : 288 -294 . DOI: 10.13995/j.cnki.11-1802/ts.032056

Abstract

The adulteration of meat products damages consumers′ trust in food quality and safety, and affects the sound development of the food industry. It has become one of the focuses of global concern. In this paper, the advantages, disadvantages, and application scenarios of the widely used detection technologies based on molecular biology principles are summarized, including qualitative general PCR, relative quantitative real-time PCR, absolute quantitative digital PCR, loop-mediated isothermal amplification for rapid detection, DNA barcoding, high-throughput sequencing, and other new molecular detection technologies. We hope this can provide theoretical reference for researchers of government regulatory authorities and third-party testing institutions.

Key words： meat products adulteration; animal-derived ingredients; molecular biology technology; high-throughput sequencing; PCR

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