木聚糖酶在造纸、酿酒等方面有广泛的应用。为了获得低成本高活力的木聚糖酶,以黑果腺肋花楸作为主要培养基原料,通过单因素试验和正交试验,探索里氏木霉和绿色木霉共发酵生产木聚糖酶的培养条件。结果表明,木聚糖酶活性最高的培养条件为氮源(NH4NO3)质量浓度0.5 g/L,果渣质量分数25%,里氏木霉与绿色木霉的质量比3∶2,接种质量分数14%,pH 5.50,培养到第4天时,其木聚糖酶活性高达121.62 U/mL。同时还探究了无机离子添加量对木聚糖酶活性的影响,无机离子的最佳添加量是MgSO4 12 mg/L和MnSO4 1.4 mg/L。结合正交试验的最佳培养条件以及最佳无机离子添加量进行发酵后,木聚糖酶的活性高达(127.25±0.09) U/mL,与基础培养基相比,木聚糖酶产量增加了69.46%。使用里氏木霉和绿色木霉共发酵黑果腺肋花楸生产木聚糖酶在获得了高活性木聚糖酶的同时降低了成本,对木聚糖酶工业化生产有重要参考价值。
Xylanase has a wide range of applications in papermaking, winemaking, etc. To obtain xylanase with low cost and high enzymatic activity, Aronia melanocarpa (Michx.) Elliott was used as the main medium material. The culture conditions for the co-fermentation of Trichoderma reesei and Trichoderma viride in Aronia melanocarpa for xylanase production were investigated through single-factor and orthogonal experiments. The results showed that the culture conditions with the highest xylanase activity were: 0.5 g/L NH4NO3 as the nitrogen source, 25% pomace (mass fraction), T. reesei∶T. viride 3∶2(mass ratio), an inoculation mass fraction of 14%, pH 5.50. Under the optimized conditions, the xylanase activity was as high as 121.62 U/mL on the fourth day of incubation. Additionally, the influence of different MgSO4, FeSO4, MnSO4, ZnCl2 ,and CoCl2 concentrations on the activity of xylanase were studied. The effect of Mg2+ and Mn2+ on the production of xylanase from the co-fermentation of T. reesei and T. viride was higher than that of Co2+, Fe2+ and Zn2+. Therefore, the optimal addition of inorganic ions was 12 mg/L MgSO4 and 1.4 mg/L MnSO4. Combining the optimal culture conditions from the orthogonal experiment and the optimal addition of inorganic ions, the xylanase activity was as high as (127.25±0.09) U/mL, an increase of 69.46%, compared to the basal medium. The co-fermentation of T. reesei and T. viride in Aronia melanocarpa to produce xylanase had low cost and high enzyme yield, which provided a valuable reference for the industrial production of xylanase.
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