食品与发酵工业 >

2023 , Vol. 49 >Issue 6: 162 - 169

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031971

研究报告

冠突散囊菌SP-5固态发酵普洱大叶毛茶产果胶酶的研究

  • 岳彦超 ,
  • 杨云娟 ,
  • 郝大海 ,
  • 黄遵锡 ,
  • 唐湘华
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  • 1(云南师范大学 生命科学学院,云南 昆明, 650500)
    2(云南省马铃薯重点实验室,云南 昆明, 650500)
    3(云南省生物质能与环境生物技术重点实验室,云南 昆明,650500)
硕士研究生(唐湘华副教授为通信作者, E-mail:txhdom@163.com)

收稿日期: 2022-04-18

  修回日期: 2022-05-29

  网络出版日期: 2023-04-14

基金资助

云南省教育厅自然科学基金资助项目(2020J0091)

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Solid-state fermentation to Pu-erh big leaf tea with Eurotium cristatum SP-5 to produce pectinase

  • YUE Yanchao ,
  • YANG Yunjuan ,
  • HAO Dahai ,
  • HUANG Zunxi ,
  • TANG Xianghua
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  • 1(College of Life Sciences, Yunnan Normal University, Kunming 650500, China)
    2(Yunnan Key Laboratory of Potato Biology, Kunming 650500, China)
    3(Key Lab of Yunnan Province for Biomass Energy and Environmental Biotechnology, Kunming 650500, China)

Received date: 2022-04-18

  Revised date: 2022-05-29

  Online published: 2023-04-14

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摘要

该文利用BIOLOG 工作站分析了冠突散囊菌对75种碳源的利用,通过显色反应筛选出可利用的碳源,以碳源结构设计多聚体底物,用于冠突散囊菌诱导培养定向产酶,筛选新酶产品。结果表明,采用果胶、木聚糖、纤维素作为碳源接种培养冠突散囊菌SP-5,其菌落形态生长良好。根据冠突散囊菌SP-5利用果胶诱导产酶特性,我们对普洱的大叶毛茶固态发酵获得果胶酶并进行了酶学性质分析。结果表明,冠突散囊菌SP-5在培养基含水量为 20%~40%的比例中长势较好,其中,在含水量 20%的茶叶培养基中产生的果胶酶活力最高,能达到12.52 U/g;果胶酶最适反应温度为 50.0 ℃,最适pH为3.50;在80 ℃条件下的半衰期为 30.28 min,在pH 2.0条件下半衰期为 15.39 min;动力学研究发现SP-5果胶酶米氏常数Km为1.21 g/L,酶促最大反应速率vmax为6.15 μmol/(U·min)。

关键词： BIOLOG工作站; 冠突散囊菌; 果胶酶; 动力学; 半衰期

本文引用格式

岳彦超 , 杨云娟 , 郝大海 , 黄遵锡 , 唐湘华 . 冠突散囊菌SP-5固态发酵普洱大叶毛茶产果胶酶的研究[J]. 食品与发酵工业, 2023 , 49(6) : 162 -169 . DOI: 10.13995/j.cnki.11-1802/ts.031971

Abstract

In the BIOLOG system, 75 kinds of carbon sources were used for Eurotium cristatum SP-5 to screen the new enzyme species. The available carbon sources were screened out through chromogenic reactions. The polymeric substrates were designed based on the carbon source structure, which could be used in the induction culture of E. cristatum for directing enzyme production. E. cristatum SP-5 was used for solid-state fermentation to produce pectinase. The results showed that pectin, xylan, and cellulose were used as carbon sources to culture SP-5, and its colony morphology grew well. Based on the pectinase induced by SP-5 with pectin, solid-state fermentation was used to produce the enzyme by SP-5 with Pu-erh big leaf tea of Yunnan, and its enzymatic dynamics were analyzed. The experiments showed that E. cristatum SP-5 grew best in the pectin medium and had the highest pectinase activity in tea medium with 20% water content, reaching 12.5 U/g. The optimum reaction temperature and pH of pectinase from SP-5 were 50 ℃ and 3.5, respectively. The half-life of pectinase's heat and acid resistance was 30.28 min at 80 ℃ and 15.3 min at pH 2.0. The Km of SP-5 pectinase was 1.21 mg/mL, and the maximum reaction rate vmax was 6.15 μmol/(U·min). Metal ion magnesium, copper, iron, calcium, potassium, and silver ions have inhibitory effects on pectinase.

Key words： BIOLOG system; Eurotium cristatum; pectinase; dynamics; half-life

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