研究报告

木枣发酵过程中三萜酸、环磷酸腺苷含量以及味觉变化研究

  • 张玲 ,
  • 张江宁
展开
  • (山西农业大学,山西功能食品研究院,山西 太原,030031)
第一作者:博士,副研究员(张江宁副研究员为通信作者,E-mail:17400495@qq.com)

收稿日期: 2022-06-09

  修回日期: 2022-06-28

  网络出版日期: 2023-05-16

基金资助

山西省科技成果转化引导专项(202104021301051);山西省农业科学院优秀青年基金项目(YCX2020YQ03)

Changes of triterpenic acid and cyclic AMP contents and taste sense during probiotics fermentation of Ziziphus jujube cv Muzao juice

  • ZHANG Ling ,
  • ZHANG Jiangning
Expand
  • (Shanxi Agricultural University, Institute of Functional Food of Shanxi, Taiyuan 030031, China)

Received date: 2022-06-09

  Revised date: 2022-06-28

  Online published: 2023-05-16

摘要

以木枣为原料,利用益生菌对枣汁进行发酵,分析发酵过程中功能活性成分环磷酸腺苷、三萜酸以及味觉的变化规律,结果表明,随着发酵时间的延长,木枣汁中熊果酸和齐墩果酸的含量逐渐上升,发酵3 d后其木枣汁中熊果酸和齐墩果酸的含量可达1.25 mg/mL。白桦脂酸的含量也逐渐上升。发酵前木枣汁中白桦脂酸的含量为2.95 mg/mL,当发酵3 d后其木枣汁中白桦脂酸的含量可达8.24 mg/mL,是发酵前木的2.8倍左右。发酵前枣汁中环磷酸腺苷的含量为0.74 mg/mL,发酵1 d后其木枣汁中环磷酸腺苷的含量可增加到1.06 mg/mL,之后其含量增加不明显。同时,该实验利用电子舌对不同发酵时间的木枣汁进行了味觉分析,随着发酵时间的延长,木枣汁的酸味值和鲜味值逐渐增加,而甜味值、咸味值和苦味值逐渐减小。经益生菌发酵后的木枣汁中三萜酸和环磷酸腺苷的含量都有所增加,其木枣汁风味也得到提升。

本文引用格式

张玲 , 张江宁 . 木枣发酵过程中三萜酸、环磷酸腺苷含量以及味觉变化研究[J]. 食品与发酵工业, 2023 , 49(8) : 145 -150 . DOI: 10.13995/j.cnki.11-1802/ts.032490

Abstract

In this experiment, Muzao (Ziziphus jujube cv Muzao) was used as raw materials and the changes of triterpenic acid and cyclic AMP contents and the taste sense during the probiotics fermentation of Muzao juice were analyzed. Results showed that with the extension of fermentation time, the total ursolic acid and oleanolic acid content increased and could reach 1.25 mg/mL after Muzao juice fermentation for 3 days. The content of betulinic acid in Muzao juice before fermentation was 2.95 mg/mL and it increased to 8.24 mg/mL after fermentation for 3 days, which was about 2.8 times of that before fermentation. The content of cyclic AMP in Muzao juice before fermentation was 0.74 mg/mL and increased to 1.06 mg/mL after fermentation for 1 day. Taste analysis showed that the value of acid and umami in Muzao juice increased gradually, while the value of sweet, salty, and bitter decreased gradually with the extension of fermentation time. The contents of triterpenic acids and cyclic AMP were increased in Muzao juice fermented by probiotics and the flavor of jujube juice was also enhanced.

参考文献

[1] 曹艳萍,杨秀利, 薛成虎.红枣中齐墩果酸提取工艺的研究[J].食品科学, 2007, 28(10):165-167.
CAO Y P, YANG X L, XUE C H.Study on extraction technology of oleanolic acid in Zizyphus jujuba date[J].Food Science, 2007, 28(10):165-167.
[2] 王文生, 姬迁龙, 杨绍艳.山西临县木枣贮藏保鲜试验研究[J].保鲜与加工, 2007, 7(4):16-18.
WANG W S, JI Q L, YANG S Y.Research of techniques of storage and fresh- keeping of muzao jujube in lin County of Shanxi Province[J].Storage and Process, 2007, 7(4):16-18.
[3] 张玲, 杨春, 丁卫英, 等. 不同品种枣的黄酮含量及其体外抗氧化性特征[J]. 贵州农业科学, 2018, 46(10):119-121.
ZHANG L, YANG C, DING W Y, et al. Flavonoids content and antioxidant activity of different jujube varieties[J]. Guizhou Agricultural Sciences, 2018, 46(10):119-121.
[4] 杨卫民, 杜京旗, 赵君.木枣多酚氧化酶酶学特性研究[J].中国食品添加剂, 2016(4):74-80.
YANG W M, DU J Q, ZHAO J.Study on the enzyme properties of polyphenol oxidase in zizyphus jujube cv.Muzao[J].China Food Additives, 2016(4):74-80.
[5] LUO M M, WANG W W, ZHAO Z H, et al.Spatiotemporal distribution of cAMP in Chinese jujube[J].Acta Horticulturae,2013(993):249-252.
[6] PAREEK S.Nutritional composition of jujube fruit[J].Emirates Journal of Food and Agriculture, 2013, 25(6):463-470.
[7] 王向红, 桑亚新, 崔同, 等.高效液相色谱法测定枣果中的环核苷酸[J].中国食品学报, 2005, 5(3):108-112.
WANG X H, SANG Y X, CUI T, et al.Determination of cyclic-nucleotide in Chinese jujube by HPLC[J].Journal of Chinese Institute of Food Science and Technology, 2005, 5(3):108-112.
[8] 盛灵慧, 高运华, 王晶, 等.固相萃取-高效液相色谱法测定枣中熊果酸和齐墩果酸[J].化学分析计量, 2008, 17(4):18-20.
SHENG L H, GAO Y H, WANG J, et al.Determination of oleanolic acid and ursolic acid in Chinese jujube by high performance liquid chromatography with solid-phase extraction[J].Chemical Analysis and Meterage, 2008, 17(4):18-20.
[9] 蔡天娇, 王瑞珍, 魏君慧, 等.白桦脂酸与红枣总三萜酸对小鼠酒精肝损伤的保护作用[J].食品科学, 2018, 39(11):191-195.
CAI T J, WANG R Z, WEI J H, et al.Protective effects of betulinic acid and total triterpenic acids from red jujubes on alcoholic liver injury in mice[J].Food Science, 2018, 39(11):191-195
[10] WEN Z M, MARTIN D E, BULLOCK P, et al.Glucuronidation of anti-HIV drug candidate bevirimat:Identification of human UDP-glucuronosyltransferases and species differences[J].Drug Metabolism and Disposition: the Biological Fate of Chemicals, 2007, 35(3):440-448.
[11] 蔡天娇. 红枣三萜酸提取纯化及其小鼠保肝作用研究[D].杨凌:西北农林科技大学, 2017.
CAI T J.Study of purification and hepatoprotective effect in mice of triterpenic acids in jujube[D].Yangling:Northwest A&F University, 2017.
[12] 付亚玲. 黑枣三萜酸的提取、分离纯化及抗氧化活性研究[D].泰安:山东农业大学, 2021.
FU Y L.Extraction, purification and antioxidant activity of triterpenic acids from blackened jujube (Zizyphus jujuba Mill.)[D].Tai'an:Shandong Agricultural University, 2021.
[13] 畅晓洁. 不同成熟度红枣的酚类物质、有机酸、三萜酸、VC含量及其抗氧化活性研究[J].保鲜与加工, 2021, 21(2):28-32.
CHANG X J.Study on contents of phenolics, organic acids, triterpene acids, vitamin C of red jujube with different maturity and their antioxidant activities[J].Storage and Process, 2021, 21(2):28-32.
[14] 李辉, 赵亮, 李建贵, 等.高效液相色谱法测定我国北方红枣中环磷酸腺苷的含量[J].分析测试技术与仪器, 2020, 26(1):42-48.
LIU H, ZHAO L, LI J G, et al.Determination of cAMP in jujube from north of China by high performance liquid chromatography[J].Analysis and Testing Technology and Instruments, 2020, 26(1):42-48.
[15] 盛洁, 田欢, 刘原野, 等.响应面法优化红枣益生菌发酵饮料工艺[J].中国酿造, 2021, 40(2):203-209.
SHENG J, TIAN H, LIU Y Y, et al.Optimization of fermentation process of probiotics jujube beverage by response surface methodology[J].China Brewing, 2021, 40(2):203-209.
[16] 胡贝多, 刘鑫磊, 战相君, 等.益生菌发酵对红枣汁抗氧化活性、营养品质及香气的影响[J].中国酿造, 2020, 39(2):44-50.
HU B D, LIU X L, ZHAN X J, et al.Effects of probiotics fermentation on antioxidant activity, nutritional quality and aroma of jujube juice[J].China Brewing, 2020, 39(2):44-50.
[17] CEJUDO BASTANTE M J, DURÁN GUERRERO E, CASTRO MEJÍAS R, et al. Study of the polyphenolic composition and antioxidant activity of new sherry vinegar-derived products by maceration with fruits[J]. Journal of Agricultural and Food Chemistry, 2010, 58(22):11 814-11 820.
[18] GUO S, DUAN J N, QIAN D W, et al.Content variations of triterpenic acid, nucleoside, nucleobase, and sugar in jujube (Ziziphus jujuba) fruit during ripening[J].Food Chemistry, 2015, 167:468-474.
[19] 高娅, 杨洁, 杨迎春, 等.不同品种红枣中三萜酸及环核苷酸的测定[J].中成药, 2012, 34(10):1 961-1 965.
GAO Y, YANG J, YANG Y C, et al.Determination of triterpenoid and cyclic-nucleotide in different Jujubae Fructus cultivars[J].Chinese Traditional Patent Medicine, 2012, 34(10):1 961-1 965.
[20] 张玲, 丁卫英, 韩基明, 等.不同品种枣粉的化学成分和电子舌分析[J].食品工业, 2020, 41(11):169-172.
ZHANG L, DING W Y, HAN J M, et al.Taste and chemical properties analysis of different kinds of jujube powder[J].The Food Industry, 2020, 41(11):169-172.
[21] 胡芳, 赵智慧, 刘孟军.HPLC法测定不同枣品种果实中白桦酯酸、齐墩果酸和熊果酸含量[J].中国农学通报, 2011, 27(5):434-438.
HU F, ZHAO Z H, LIU M J.The determination of betulic acid, oleanic acid and ursolic acid in different Chinese jujube cultivars by HPLC[J].Chinese Agricultural Science Bulletin, 2011, 27(5):434-438.
[22] 王向红, 崔同, 齐小菊, 等.HPLC法测定不同品种枣及酸枣中的齐墩果酸和熊果酸[J].食品科学, 2002,23(6):137-138.
WANG X H, CUI T, QI X J, et al.Determination of ursolic acid and ol eanolic acid in jujuba and wild jujuba by HPLC[J].Food Science, 2002,23(6):137-138.
[23] 梁鑫, 陈思雨, 赵育, 等.乳酸菌和酵母菌发酵红枣汁工艺优化及成分分析[J].食品与发酵工业, 2021, 47(7):175-182.
LIANG X, CHEN S Y, ZHAO Y, et al.Process optimization and composition analysis of jujube juice fermentation by lactic acid bacteria and yeast[J].Food and Fermentation Industries, 2021, 47(7):175-182.
[24] 谢明勇, 熊涛, 关倩倩.益生菌发酵果蔬关键技术研究进展[J].中国食品学报, 2014, 14(10):1-9.
XIE M Y, XIONG T, GUAN Q Q.Research progress on the key techniques of the fruit and vegetable products fermented by probiotics[J].Journal of Chinese Institute of Food Science and Technology, 2014, 14(10):1-9.
[25] 陈历水, 丁庆波, 吴伟莉, 等.发酵果蔬汁的功能特性的研究进展[J].食品工业科技, 2012, 33(11):418-421;425.
CHEN L S, DING Q B,WU W L, et al.Research progress in functional characteristics of fermented fruit and vegetable juices[J].Science and Technology of Food Industry, 2012, 33(11):418-421;425.
[26] 全琦, 刘伟, 左梦楠, 等.乳酸菌发酵果蔬汁的风味研究进展[J].食品与发酵工业, 2022, 48(1):315-323.
QUAN Q, LIU W, ZUO M N, et al.Advances in the flavor of fruit and vegetable juices fermented by lactic acid bacteria[J].Food and Fermentation Industries, 2022, 48(1):315-323.
文章导航

/