为提高樟芝菌液态发酵硒多糖产量,探究樟芝菌多糖富硒后活性方面的变化。以生物量、多糖含量、硒含量和富硒率为评价指标,利用单因素试验和正交试验对发酵条件进行优化,综合得出最佳发酵条件。同时在脂多糖(lipopolysaccharide,LPS)诱导小鼠腹腔巨噬细胞(RAW264.7)建立炎症模型的基础上,评价樟芝菌硒多糖与无硒多糖的抗炎活性。通过发酵优化得到最佳发酵条件为Na2SeO3添加量2 mg/L、麦芽糖24 g/L、可溶性淀粉16 g/L、玉米浆干粉9 g/L,硫酸铵9 g/L,装液量75 mL/250 mL、初始pH值7,24 ℃、150 r/min发酵9 d。体外细胞实验结果表明,二者均能够显著降低促炎介质NO、TNF-α、IL-6和IL-1β的释放量,提高细胞内超氧化物歧化酶(superoxide dismutase,SOD)活性,起到有效抑制炎症反应的作用。而且,硒多糖在提高SOD活性、抑制IL-1β释放方面效果要优于无硒多糖。综上,樟芝菌硒多糖在富硒的同时,能够具有高于无硒多糖的抗炎活性,具备一定的开发前景。
To improve the production of selenium polysaccharide from submerged fermentation of Antrodia cinnamomea, and to explore the changes of polysaccharide activity after selenium enrichment. Biomass, polysaccharide content, selenium content and selenium enrichment rate were used as evaluating indicator. The fermentation conditions of polysaccharide were optimized by single factor experiment and orthogonal experiment. Hence, the best fermentation conditions were obtained. Meanwhile, on the basis of establishing inflammatory model of mouse peritoneal macrophages (RAW264.7) induced by lipopolysaccharide (LPS), the anti-inflammatory activities of selenium polysaccharide and selenium-free polysaccharide of A. cinnamomea were evaluated. Through fermentation optimization, the optimum fermentation conditions were obtained as follows: Na2SeO3 2 mg/L, maltose 24 g/L, soluble starch 16 g/L, corn pulp dry powder 9 g/L, ammonium sulfate 9 g/L, liquid volume 75 mL/250 mL, initial pH 7, fermentation 9 d at 24 ℃, 150 r/min. The results of cell experiment in vitro showed that both of them could significantly reduce the expression of pro-inflammatory mediators such as nitrite oxide (NO), tumor necrosis factor (TNF-α), interleukin IL-6 and IL-1β, increase the activity of intracellular superoxide dismutase (SOD), and effectively inhibit the inflammatory reaction. Moreover, selenium polysaccharide is superior to selenium-free polysaccharide in increasing SOD activity and inhibiting IL-1β expression. In summary, while enriching selenium, selenium polysaccharide from A. cinnamomea have higher anti-inflammatory activity than selenium-free polysaccharide, which has a certain development prospect.
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