食品与发酵工业 >

2023 , Vol. 49 >Issue 9: 158 - 163

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031463

研究报告

熊果酸壳聚糖纳米粒子的体外消化特性及稳定性

  • 赵路洁 ,
  • 段续 ,
  • 曹伟伟 ,
  • 任广跃 ,
  • 李琳琳 ,
  • 刘盼盼 ,
  • 任星 ,
  • 苗峻伟 ,
  • BHESH Bhandari ,
  • 陈俊亮
展开
  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471023)
    2(粮食储藏安全河南省协同创新中心,河南 郑州,450001)
    3(昆士兰大学 农业和食品科学学院,昆士兰州 布里斯班市,4702)
硕士研究生(段续教授为通信作者,E-mail:duanxu_dx@163.com)

收稿日期: 2022-03-08

  修回日期: 2022-04-06

  网络出版日期: 2023-06-05

基金资助

河南省引进国外智力专项计划项目(HNGD2021040);河南省科技攻关项目(212102110077)

收起

In vitro digestion characteristics and stability of ursolic acid-loaded chitosan nanoparticles

  • ZHAO Lujie ,
  • DUAN Xu ,
  • CAO Weiwei ,
  • REN Guangyue ,
  • LI Linlin ,
  • LIU Panpan ,
  • REN Xing ,
  • MIAO Junwei ,
  • BHESH Bhandari ,
  • CHEN Junliang
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Collaorative Innovation Center of Grain Storage Security (Henan Province), Zhengzhou 450001, China)
    3(School of Agriculture and Food Sciences, University of Queensland, Brisbane 4702, Australia)

Received date: 2022-03-08

  Revised date: 2022-04-06

  Online published: 2023-06-05

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摘要

分别采用冷冻干燥、微波冷冻干燥和喷雾干燥方式制备熊果酸-壳聚糖纳米粒子,研究了熊果酸纳米粒子在光照、不同温度及体外模拟胃肠消化过程中的贮藏稳定性和抗氧化能力。结果表明,胃消化阶段,熊果酸纳米粒子释放率较少;肠消化阶段,冷冻干燥、微波冷冻干燥和喷雾干燥制备的熊果酸纳米粒子释放率分别为59%、55%和50%,说明熊果酸纳米粒子具有缓释特性。体外模拟胃肠消化阶段的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2- picrylhydrazyl,DPPH)自由基清除率试验结果表明,熊果酸纳米粒子的抗氧化性高于熊果酸。熊果酸和熊果酸纳米粒子在4 ℃下稳定性较高,熊果酸保留率均在80%以上。避光室温条件下,冷冻干燥、微波冷冻干燥和喷雾干燥制备的熊果酸纳米粒子保留率分别为66%、64%和60%。其中,冷冻干燥制备的熊果酸纳米粒子在4 ℃和避光条件下DPPH自由基清除率最高,分别为2.32、2.09 mg/g。研究结果表明,冷冻干燥和微波冷冻干燥制备的熊果酸纳米粒子的光照、温度以及模拟胃肠消化稳定性和抗氧化性均高于熊果酸,熊果酸的稳定性显著提高。

关键词： 熊果酸; 壳聚糖纳米粒子; 稳定性; 抗氧化性

本文引用格式

赵路洁 , 段续 , 曹伟伟 , 任广跃 , 李琳琳 , 刘盼盼 , 任星 , 苗峻伟 , BHESH Bhandari , 陈俊亮 . 熊果酸壳聚糖纳米粒子的体外消化特性及稳定性[J]. 食品与发酵工业, 2023 , 49(9) : 158 -163 . DOI: 10.13995/j.cnki.11-1802/ts.031463

Abstract

Ursolic acid (UA)-loaded chitosan nanoparticles were prepared by freeze drying (FD), microwave freeze drying (MFD), and spray drying (SD), and the storage stability and antioxidant activity of UA in UA-loaded chitosan nanoparticles in light, temperature, and in vitro simulated gastrointestinal digestion process were investigated. Results showed that the release rate of UA-loaded chitosan nanoparticles was less in the gastric digestion stage. During the intestinal digestion stage, the release rates of UA-loaded chitosan nanoparticles prepared by FD, MFD, and SD were 59%, 55%, and 50%, respectively. It showed that UA-loaded chitosan nanoparticles had sustained release properties. The results of the DPPH free radical scavenging activity in simulating the gastrointestinal digestion stage showed that the antioxidant activities of UA-loaded chitosan nanoparticles were higher than those of UA. UA and UA-loaded chitosan nanoparticles had high stability at 4 ℃, and the retention rate of UA was greater than 80%. Under light protection conditions, the retention rates of UA-loaded chitosan nanoparticles prepared by FD, MFD, and SD were 66%, 64%, and 60%, respectively. Among them, the UA-loaded chitosan nanoparticles prepared by FD had the highest activity of DPPH free radical scavenging at 4 ℃ and avoidance of light, at 2.32 and 2.09 mg/g, respectively. The stability and antioxidant activities of UA-loaded chitosan nanoparticles prepared by FD and MFD under the light, temperature, and simulated gastrointestinal digestion were higher than those of UA-loaded chitosan nanoparticles, and the stability of UA was significantly improved.

Key words： ursolic acid; chitosan nanoparticles; stability; antioxidant activity

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