食品与发酵工业 >

2023 , Vol. 49 >Issue 12: 209 - 214

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033064

研究报告

基于宏基因组分箱方法揭示房县黄酒加工用小曲中微生物多样性

  • 王玉荣 ,
  • 李文鹏 ,
  • 蔡文超 ,
  • 刘慧杰 ,
  • 余海燕 ,
  • 侯强川 ,
  • 郭壮
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  • 1(湖北文理学院,湖北省食品配料工程技术研究中心,湖北 襄阳,441053)
    2(湖北文理学院,乳酸菌生物技术与工程襄阳市重点实验室,湖北 襄阳,441053)
    3(北京忠和房县生物食品有限公司,湖北 十堰,442100)
    4(湖北庐陵王酒业有限责任公司,湖北 十堰,442100)
博士,讲师(郭壮教授为通信作者,E-mail:guozhuang1984@163.com)

收稿日期: 2022-07-21

  修回日期: 2022-07-30

  网络出版日期: 2023-07-13

基金资助

湖北省教育厅科学技术研究重点项目(D20212601);湖北文理学院教师科研能力培育基金“科技创新团队”(2020kypytd009)

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Revealing microbial diversity in Huangjiu Xiaoqu by metagenomic binning approaches

  • WANG Yurong ,
  • LI Wenpeng ,
  • CAI Wenchao ,
  • LIU Huijie ,
  • YU Haiyan ,
  • HOU Qiangchuan ,
  • GUO Zhuang
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  • 1(Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China)
    2(Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang 441053, China)
    3(Beijing Zhonghe Fanxian Bio-Food Co.Ltd., Shiyan 442100, China)
    4(Hubei Lulingwang Wine Industry Co.Ltd., Shiyan 442100, China)

Received date: 2022-07-21

  Revised date: 2022-07-30

  Online published: 2023-07-13

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摘要

该研究以房县黄酒加工用小曲为研究对象,使用宏基因组分箱(Binning)技术对其微生物多样性进行解析。在每个样本平均获得148 458 867条高质量序列,平均测序量为20.74 Gb的基础上发现,硬壁菌门(Firmicutes)、变形菌门(Proteobacteria)和子囊菌门(Ascomycota)为房县黄酒加工用小曲中的优势菌门,累计占比为99.06%;而戊糖片球菌(Pediococcus pentosaceus,24.04%)、食窦魏斯氏菌(Weissella cibaria,18.86%)和融合魏斯氏菌(Weissella confusa,16.15%)等菌种为优势种。该研究通过宏基因组Binning技术从宏基因组序列中共获得8个高质量的宏基因组组装基因组(metagenome assembly genomes,MAGs),分别隶属于硬壁菌门下的5个菌属。此外,通过ANI(average nucleotide identity)分析从高质量MAGs中发现两个疑似的新物种,分别为Weissella sp.和乳杆菌(Lactobacillus sp.)。基因功能注释结果表明,Lactobacillus sp.具有较高的碳水化合物运输和代谢能力,并含有许多独有的碳水化合物运输和代谢基因,可能促进房县黄酒的发酵进程。由此可见,在后续研究中对房县黄酒加工用小曲中蕴含的微生物资源进行挖掘是极为必要的。

关键词： 房县; 黄酒曲; 宏基因组; 宏基因组组装; 微生物多样性

本文引用格式

王玉荣 , 李文鹏 , 蔡文超 , 刘慧杰 , 余海燕 , 侯强川 , 郭壮 . 基于宏基因组分箱方法揭示房县黄酒加工用小曲中微生物多样性[J]. 食品与发酵工业, 2023 , 49(12) : 209 -214 . DOI: 10.13995/j.cnki.11-1802/ts.033064

Abstract

In this study, the microbial diversity of Xiaoqu of Huangjiu in Fangxian region was resolved using metagenomic binning technology.Based on an average of 148 458 867 high-quality sequences and an average sequencing volume of 20.74 Gb per sample, it was found that Firmicutes, Proteobacteria and Ascomycota were the dominant phyla in Huangjiu Xiaoqu with a cumulative percentage of 99.06%, while Pediococcus pentosaceus (24.04%), Weissella cibaria (18.86%), and Weissella confusa (16.15%) were the dominant species.A total of eight high-quality metagenome assembly genome (MAGs) belonging to five genera of Firmicutes were obtained from the metagenomic sequence by binning.In addition, two suspected new species, Weissella sp.and Lactobacillus sp, were identified from the high-quality MAGs by average nucleotide identity (ANI) analysis.The results of gene function annotation indicated that Lactobacillus sp.has high carbohydrate transport and metabolism ability and contains many unique carbohydrate transport and metabolism genes, which may promote the fermentation process of Huangjiu.Thus, it is extremely necessary to explore the microbial resources contained in the Xiaoqu used in the processing of Huangjiu in Fangxian region in subsequent studies.

Key words： Fangxian; Huangjiu Xiaoqu; metagenome; metagenome binning; microbial diversity

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