食品与发酵工业 >

2023 , Vol. 49 >Issue 12: 278 - 284

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033093

分析与检测

超高效液相色谱质谱法测定酱香型酒曲原料中氨基酸含量

  • 乔敏莎 ,
  • 王凡 ,
  • 山其木格 ,
  • 乔晓娜 ,
  • 毕荣宇 ,
  • 张长霞 ,
  • 卢君
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  • 1(天士力医药集团股份有限公司研究院,天津,300410)
    2(贵州国台酒业集团股份有限公司,贵州 仁怀,564501)
硕士,中级工程师(卢君高级工程师为通信作者,E-mail:lujun951@tasly.com)

收稿日期: 2022-07-25

  修回日期: 2022-08-18

  网络出版日期: 2023-07-13

基金资助

贵州省科技成果应用及产业化计划项目(黔科合成果[2020]2Y045);贵州省仁怀市科技计划项目(仁科支撑[2020]04号);遵义市科技计划项目(遵市科合R&D[2020]31号;遵市科合支撑GY[2021]40号)

收起

Direct determination of 19 amino acids in raw materials of Moutai-flavor Jiuqu by ultra performance liquid chromatography-tandem mass spectrometry

  • QIAO Minsha ,
  • WANG Fan ,
  • SHAN Qimuge ,
  • QIAO Xiaona ,
  • BI Rongyu ,
  • ZHANG Changxia ,
  • LU Jun
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  • 1(Tasly Academy, Tasly Holding Group Co.Ltd., Tianjin 300410, China)
    2(Guizhou Guotai Distillery Co.Ltd., Renhuai 564501, China)

Received date: 2022-07-25

  Revised date: 2022-08-18

  Online published: 2023-07-13

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摘要

利用超高效液相色谱-质谱联用仪(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)建立了一种高效、快速测定酱香型白酒酿造大曲及制曲原料小麦中19种游离氨基酸的方法,并对酱酒制曲过程中氨基酸的变化规律进行初步探究。样品经粉碎、超声提取、离心及适当稀释后,可直接用于含量分析。结果表明,19种游离氨基酸在特定范围内线性关系良好(R2≥0.998 3),检出限为0.3~6.1 ng/mL,定量限为2.1~29.3 ng/mL,加标回收率为84.5%～118.5%,方法重复性和仪器精密度相对标准偏差均低于6.5%(n=6)。该方法具有分析速度快、灵敏度高且选择性好的优点,适用于酱香型白酒酿造大曲及原料中19种游离氨基酸的定量分析。

关键词： 氨基酸; 检测; 超高效液相色谱-质谱法; 酱香型酒曲

本文引用格式

乔敏莎 , 王凡 , 山其木格 , 乔晓娜 , 毕荣宇 , 张长霞 , 卢君 . 超高效液相色谱质谱法测定酱香型酒曲原料中氨基酸含量[J]. 食品与发酵工业, 2023 , 49(12) : 278 -284 . DOI: 10.13995/j.cnki.11-1802/ts.033093

Abstract

An efficient and rapid method was established by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of 19 free amino acids in Moutai-flavor Daqu starter and its raw material -wheat.And the variation tendency of these amino acids in the production process of Moutai-flavor Daqu starter was preliminarily analyzed.The sample was for direct content determination after crushing, ultrasonic extraction, centrifugation and appropriate dilution.The results showed that the linear relationship of these 19 free amino acids was good within a specific range (R2≥0.998 3), the limit of detection (LOD) was 0.3-6.1 ng/mL, the limit of quantitation (LOQ) was 2.1-29.3 ng/mL, and the recovery rate was 84.5%-118.5%.The relative standard deviation values of repeatability and instrument precision were both less than 6.5% (n=6).The method has the advantages of high speed, high sensitivity and good selectivity and is suitable for the quantitative analysis of 19 free amino acids in Moutai-flavor Daqu starter and its raw material.

Key words： amino acids; determination; ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); Moutai-flavor Jiuqu

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