食品与发酵工业 >

2023 , Vol. 49 >Issue 13: 239 - 249

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033528

研究报告

构建原位集气袋气相色谱质谱测定苹果香气并预测其品质变化

  • 曹晓蒙 ,
  • 杜国荣 ,
  • 赵鹏涛 ,
  • 刘慧 ,
  • 赵擎豪 ,
  • 成晨亚琼 ,
  • 王晓宇
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  • 1(陕西师范大学 食品工程与营养科学学院,陕西 西安,710119)
    2(西安文理学院 生物与环境工程学院,陕西 西安,710065)
    3(西部果品资源高值利用教育部工程研究中心,陕西 西安,710119)
第一作者:硕士研究生(王晓宇教授为通信作者,E-mail:wangxiaoyu@snnu.edu.cn)

收稿日期: 2022-09-03

  修回日期: 2022-10-08

  网络出版日期: 2023-08-07

基金资助

陕西省重点研发计划-重点产业创新链项目(2020ZDLNY05-08);咸阳市科技局项目(2021ZDYF-NY-0021)

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Designing in situ air collecting bag to detect apple aroma and predict its quality change

  • CAO Xiaomeng ,
  • DU Guorong ,
  • ZHAO Pengtao ,
  • LIU Hui ,
  • ZHAO Qinghao ,
  • CHENG Chenyaqiong ,
  • WANG Xiaoyu
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  • 1(College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710119, China)
    2(College of Biological and Environmental Engineering, Xi'an Academy of Arts and Sciences, Xi'an 710065, China)
    3(Engineering Research Center of High-Value Utilization of Western China Fruit Resources, Ministry of Education, Xi'an 710119, China)

Received date: 2022-09-03

  Revised date: 2022-10-08

  Online published: 2023-08-07

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摘要

为建立新的苹果品质评价标准和寻找苹果标志性香气化合物,该文利用集气袋收集苹果逸散香气,同时结合顶空固相微萃取-气相色谱质谱联用和电子鼻分析技术,对苹果香气组分进行分析。结果发现,利用原位集气袋收集到苹果逸散香气47种,果体自留香气有30种,峰面积值较高的包括2-甲基丁酸己酯、己酸己酯、乙酸己酯、乙酸2-甲基丁酯和2-甲基丁酸丁酯。气味活度值分析发现,随着货架期延长,苹果主要呈香物质数量减少。热图聚类分析与偏最小二乘-判别分析可根据特征香气变化区分其品质,通过变量重要性因子分析寻找出1-丁醇、2-甲基-1-丁醇、乙酸2-甲基丁酯为潜在标志性香气。电子鼻结果显示不同货架期在LDA2可被显著区分。该实验为苹果采后贮藏和流通过程中香气品质变化提供了可行的检测方法。

关键词： 货架期; 多元组学分析; 果体自留香气; 苹果逸散香气; 电子鼻分析

本文引用格式

曹晓蒙 , 杜国荣 , 赵鹏涛 , 刘慧 , 赵擎豪 , 成晨亚琼 , 王晓宇 . 构建原位集气袋气相色谱质谱测定苹果香气并预测其品质变化[J]. 食品与发酵工业, 2023 , 49(13) : 239 -249 . DOI: 10.13995/j.cnki.11-1802/ts.033528

Abstract

To establish a new apple quality evaluation standard and find the apple's iconic aroma compounds, this paper collected the apple's escaped aroma using a gas bag, and combined headspace solid-phase microextraction gas chromatography-mass spectrometry and electronic nose analysis technology to analyze the apple's aroma compounds. Results showed that 47 aroma species were collected by in situ airbags, and the highest peak area values were hexyl 2-methyl butyrate, hexyl hexanoate, hexyl acetate, 2-methyl butyl acetate, and 2-methyl butyl butyrate, however, the retention aroma of apple are 30 kinds. Analysis of odor activity value showed that the number of aroma compounds that played a major role in apples decreased with the increase in shelf life. Thermal map clustering analysis and partial least squares regression could be used to distinguish the shelf life according to the characteristic aroma changes, and 1-butanol, 2-methyl-1-butanol, and 2-methyl-butyl acetate were screened as potential signature aromas by variable importance factor analysis. Electronic nose results showed that different shelf life in LDA2 could be distinguished significantly. This study provided a feasible method for the detection of aroma quality changes in apples during storage and circulation after harvest.

Key words： shelf life; multivariate omics analysis; fruit retention aroma; the escaping aroma of apple; electronic nose analysis

参考文献

[1] ESPINO-DÍAZ M, SEPÚLVEDA D R, GONZÁLEZ-AGUILAR G, et al.Biochemistry of apple aroma:A review[J].Food Technology and Biotechnology, 2016, 54(4):375-397.
[2] 匡立学, 聂继云, 李银萍, 等.中国不同地区‘富士'苹果品质评价[J].中国农业科学, 2020, 53(11):2253-2263.
KUANG L X, NIE J Y, LI Y P, et al.Quality evaluation of ‘Fuji' apples cultivated in different regions of China[J].Scientia Agricultura Sinica, 2020, 53(11):2253-2263.
[3] CAO Y D, WANG H J, WU W B, et al. Simple and effctive chracterization of fuji apple flavor quality by ethylene and sugar content[J]. Food Analytical Methods, 2021, 14(12): 2576-2784.
[4] WAGHMODE B, MASOODI L, KUSHWAHA K, et al.Volatile components are non-invasive biomarkers to track shelf-life and nutritional changes in apple cv.‘Golden Delicious' during low-temperature postharvest storage[J].Journal of Food Composition and Analysis, 2021, 102:104075.
[5] 路翔, 高源, 王昆, 等.苹果栽培品种不同族系香气特征分析[J].中国农业科学, 2022, 55(3):543-557.
LU X, GAO Y, WANG K, et al.Analysis of aroma characteristics in different cultivated apple strains[J].Scientia Agricultura Sinica, 2022, 55(3):543-557.
[6] 杜薇, 陈丽娟, 李瑞, 等.5种苹果野生资源果实香气物质的分析[J].西北农业学报, 2021, 30(10):1547-1555.
DU W, CHEN L J, LI R, et al.Analysis of aroma components in five wild apple resources[J].Acta Agriculturae Boreali-Occidentalis Sinica, 2021, 30(10):1547-1555.
[7] 马蒙蒙, 程晨霞, 杨绍兰, 等.富士系不同品种苹果货架期间品质特性分析[J].青岛农业大学学报(自然科学版), 2020, 37(2):84-90;94.
MA M M, CHENG C X, YANG S L, et al.Analysis of the fruit shelf life quality of different ‘Fuji' apple cultivars[J].Journal of Qingdao Agricultural University (Natural Science), 2020, 37(2):84-90;94.
[8] 程超, 夏兰欣, 杜芬妮, 等.基于9种风味物质的利川红SPME优化及PLS-DA风味识别[J].食品科学, 2021, 42(6):215-222.
CHENG C, XIA L X, DU F N, et al.Optimization of solid phase microextraction for GC-MS analysis of nine flavor compounds in Lichuan Hong tea and flavor discrimination by partial least squares-discriminant analysis[J].Food Science, 2021, 42(6):215-222.
[9] 张乐, 张雅, 史冠莹, 等. GC-IMS结合化学计量学分析8个产区香椿挥发性成分差异[J]. 食品科学, 2022, 43(22):301-308.
ZHANG L, ZHANG Y, SHI G Y, et al.Differences of volatile organic compounds of Toona sinensis from 8 regions analyzed by GC-IMS Combined with Chemometrics[J].Food Science, 2022, 43(22):301-308.
[10] 任晓俊. 苹果采后风味物质变化及货架期模型构建[D].锦州: 渤海大学, 2019.
REN X J.Changes of flavor compounds and establishment shelf-life model in post-harvest apple[D].Jinzhou:Bohai University, 2019.
[11] 杨绍兰, 杨玉群, 张新富, 等.1-甲基环丙烯和乙酰水杨酸处理对茌梨果实质构性能的影响[J].北方园艺, 2012(5):1-4.
YANG S L, YANG Y Q, ZHANG X F, et al.Effect of 1-MCP and ASA treatments on the fruit texture profile of ‘chili' pear[J].Northern Horticulture, 2012(5):1-4.
[12] QI Y W, LEI Q, ZHANG Y J, et al.Comparative transcripome data for commercial maturity and physiological maturity of ‘Royal Gala' apple fruit under room temperature storage condition[J].Scientia Horticulturae, 2017, 225:386-393.
[13] 林莎莎, 张琦, 于巨, 等.不同桃品种果皮色差值与色素含量的关系分析[J].江西农业学报, 2018, 30(9):35-38.
LIN S S, ZHANG Q, YU J, et al.Analysis of relationship between chromatic aberration value and pigment content of peel in different peach varieties[J].Acta Agriculturae Jiangxi, 2018, 30(9):35-38.
[14] YAN D, SHI J R, REN X L, et al.Insights into the aroma profiles and characteristic aroma of ‘Honeycrisp' apple (Malus ×domestica)[J].Food Chemistry, 2020, 327:127074.
[15] BIZZIO L N, TIEMAN D, MUNOZ P R.Branched-chain volatiles in fruit:A molecular perspective[J].Frontiers in Plant Science, 2022, 12:814138.
[16] EL HADI M A M, ZHANG F J, WU F F, et al. Advances in fruit aroma volatile research[J]. Molecules, 2013, 18(7):8200-8229.
[17] DONADEL J Z, THEWES F R, ANESE R O, et al.Key volatile compounds of ‘Fuji Kiku' apples as affected by the storage conditions and shelf life:Correlation between volatile emission by intact fruit and juice extracted from the fruit[J].Food Research International (Ottawa, Ont.), 2019, 125:108625.
[18] 何近刚, 冯云霄, 程玉豆, 等.采后1-MCP和MAP处理对‘红富士'苹果冷藏和货架期品质的影响[J].食品科学, 2016, 37(22):301-306.
HE J G, FENG Y X, CHENG Y D, et al.Effects of postharvest 1-MCP and MAP treatments on physiological characteristics and quality of ‘Fuji' apple during cold storage and subsequent shelf life[J].Food Science, 2016, 37(22):301-306.
[19] 李莹. 基于电子鼻的苹果品质无损检测研究[D].杨凌: 西北农林科技大学, 2014.
LI Y.Non-destructive testing of apple qaulity based on electronic nose[D].Yangling:Northwest A&F University, 2014.
[20] 张雅. 温度和乙烯处理对‘富士'苹果采后果实品质的影响[D].泰安: 山东农业大学, 2020.
ZHANG Y.Effects of temperature and ethylene treatment on postharvest fruit quality of ‘Fuji' apple[D].Tai'an:Shandong Agricultural University, 2020.
[21] 倪瑞洁, 詹萍, 田洪磊.基于GC-IMS结合多元统计方法分析炸制时间对花椒调味油挥发性物质的影响[J].食品科学, 2022, 43(6):279-286.
NI R J, ZHAN P, TIAN H L.Effects of frying time on volatile flavor compounds in fried pepper(Zanthoxylum bungeanum) oil as analyzed by gas chromatography-ion mobility spectrometry and multivariate statistical analysis[J].Food Science, 2022, 43(6):279-286.
[22] LIU Y D, TANG M F, LIU M C, et al. The molecular regulation of ethylene in fruit ripening[J]. Small Methods, 2020, 4(8):1900485.
[23] 张敏. 不同贮藏条件对富士及寒富苹果的货架品质调查[D].沈阳: 辽宁大学, 2019.
ZHANG M.Investigation on the shelf quality of Fuji and hanfu apples under different storage conditions[D].Shenyang:Liaoning University, 2019.
[24] PAN X, WU J H, ZHANG W T, et al.Effects of sugar matrices on the release of key aroma compounds in fresh and high hydrostatic pressure processed Tainong mango juices[J].Food Chemistry, 2021, 338:128117.
[25] 张薇薇. 富士苹果不同贮藏条件下品质变化和消费者接受度差异研究[D].哈尔滨: 东北农业大学, 2018.
ZHANG W W.Study on the difference of quality and consumer acceptance of fuji apple under different storage conditions[D].Harbin:Northeast Agricultural University, 2018.
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