食品与发酵工业 >

2023 , Vol. 49 >Issue 15: 160 - 165

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033590

研究报告

乌鸡肽中潜在功能活性肽段的研究

  • 方磊 ,
  • 李诒光 ,
  • 陈亮 ,
  • 刘丹 ,
  • 王雨晴 ,
  • 廖群 ,
  • 张新雪 ,
  • 许锦珍 ,
  • 刘文君 ,
  • 刘文颖
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  • 1(中国食品发酵工业研究院有限公司,北京市蛋白功能肽工程技术研究中心,北京,100015)
    2(江中药业股份有限公司,江西 南昌,330096)
第一作者:硕士,工程师(刘文君高级工程师和刘文颖高级工程师为共同通信作者,E-mail:liuwenjun@crjz.com;wenyingliu888@126.com)

收稿日期: 2022-09-09

  修回日期: 2022-10-24

  网络出版日期: 2023-08-31

基金资助

宁夏回族自治区重点研发计划项目(2021BEG02027);北京市科技创新基地培育与发展工程专项(Z191100002819001)

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Potential functional active peptides from silky fowl peptides

  • FANG Lei ,
  • LI Yiguang ,
  • CHEN Liang ,
  • LIU Dan ,
  • WANG Yuqing ,
  • LIAO Qun ,
  • ZHANG Xinxue ,
  • XU Jinzhen ,
  • LIU Wenjun ,
  • LIU Wenying
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  • 1(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Engineering Research Center of Protein and Functional Peptides, Beijing 100015, China)
    2(Jiangzhong Pharmaceutical Co.Ltd., Nanchang 330096, China)

Received date: 2022-09-09

  Revised date: 2022-10-24

  Online published: 2023-08-31

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摘要

研究考察了乌鸡肽的总蛋白、肽含量、氨基酸组成、分子质量分布以及潜在的功能活性肽段。结果表明,乌鸡肽的总蛋白含量(干基)为94.88%,肽含量(干基)为78.93%,氨基酸组成丰富,分子质量主要分布在1 000 Da以下,占比91.04%,此外,基于超快速液相色谱串联三重四极杆质谱方法,从乌鸡肽中鉴定出5条潜在功能活性的特征肽段序列,分别为谷氨酰精氨酸二肽、异亮氨酰精氨酸二肽、天冬酰胺酰精氨酸二肽、脯氨酰天冬氨酰精氨酸三肽、丙氨酰丙氨酰精氨酸三肽,其含量为0.14~4.28 mg/g,研究结果为乌鸡肽应用于营养保健食品奠定了物质基础。

关键词： 乌鸡肽; 分子质量分布; 氨基酸组成; 肽段鉴定; 质谱

本文引用格式

方磊 , 李诒光 , 陈亮 , 刘丹 , 王雨晴 , 廖群 , 张新雪 , 许锦珍 , 刘文君 , 刘文颖 . 乌鸡肽中潜在功能活性肽段的研究[J]. 食品与发酵工业, 2023 , 49(15) : 160 -165 . DOI: 10.13995/j.cnki.11-1802/ts.033590

Abstract

The total protein, peptide content, amino acid composition, molecular weight distribution, and potential functional active peptides of silky fowl peptides were investigated. Results showed that the total protein content (dry basis) of silky fowl peptides was 94.88%, the peptide content (dry basis) was 78.93%, the amino acid composition was rich, and the molecular mass was mainly distributed below 1 000 Da, accounting for 91.04%. In addition, 5 characteristic peptide components with potential functional activities were identified from silky fowl peptides by ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry method, including glutyl-arginine dipeptide (ER), isoleucyl arginine dipeptide (IR), asparagyl-arginine dipeptide (NR), prolyl-aspartyl-arginine tripeptide (PDR), and alanyl-alanyl-arginine tripeptide (AAR), the content of which was 0.14-4.28 mg/g, laying a material foundation for the application of silky fowl peptides to nourishing blood in nutritious and healthy food.

Key words： silky fowl peptides; molecular weight distribution; amino acid composition; peptide identification; mass spectrometry

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