采用NaOH溶液协同乳酸溶液作为处理剂,以明暗度和质量损失率为指标,研究了NaOH溶液与乳酸溶液的pH和浸泡处理时间对贻贝壳表面角质层脱除作用的影响,得出了pH 13的NaOH溶液浸泡处理36 h,再经pH 2乳酸溶液浸泡处理4 h为最佳净化处理条件。对净化处理后样品的扫描电镜、傅里叶变换红外光谱、电感耦合等离子体质谱等检测显示,NaOH-乳酸协同处理有效脱除了贻贝壳表面的黑色素和黏连角质的低纯CaCO3,及角质层吸附的Br、Zn、Pb等元素,贝壳表面钙含量从对照组的0.39%上升到37.58%,已近似达到CaCO3中理论钙含量。该研究为制备高品质贻贝壳源钙补充剂提供了一定的理论参考。
In this study, the Mytilus edulis shell was treated with NaOH-lactic acid. The effects of the pH and immersion time of NaOH and lactic acid solution on the removal of cuticles on Mytilus edulis shell were investigated by using the mass loss rate and the brightness of the shell powder as the indicators. It was found that the optimal treatment conditions were as follows: immersing in NaOH solution (pH 13) for 36 h, and then immersing in lactic acid solution (pH 2) for 4 h. The results of scanning electron microscope, Fourier transform infrared spectroscopy, and inductively coupled plasma mass spectrometry analysis showed that NaOH-lactic acid treatment effectively removed the melanin and the impurified CaCO3 adjoining with the cuticles on the shell, and also the elements adsorbed on the cuticles, such as Br, Zn, and Pb. The mass ratio of Ca of M. edulis shell surface was increased from 0.39% in control to 37.58% after NaOH-lactic acid treatment, which was approximately equal to the theoretical value in CaCO3. This study provides a reference for the preparation of high-quality calcium supplements from the M. edulis shell.
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