研究报告

低盐咸鸭蛋腌制过程对蛋黄中游离氨基酸和风味的影响

  • 何春霞 ,
  • 王修俊 ,
  • 于沛 ,
  • 包欢欢 ,
  • 许九红 ,
  • 李佳敏 ,
  • 冯廷萃
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  • 1(贵州大学 酿酒与食品工程学院,贵州 贵阳,550025)
    2(贵州省发酵工程与生物制药重点实验室,贵州 贵阳,550025)
    3(镇远县名城食品厂,贵州 黔东南苗族侗族自治州,557700)
第一作者:硕士研究生(王修俊教授为通信作者,E-mail:775298123@qq.com)

收稿日期: 2022-08-04

  修回日期: 2022-11-12

  网络出版日期: 2023-08-31

基金资助

贵州省科技计划项目(黔科合支撑[2017]2555);贵州省朝天椒产业集群建设项目(黔农函[2020]43号);贵州省科技计划重点项目(黔科合支撑[2022]重点010号);中央引导地方科技发展专项(黔科中引地[2018]4020);贵州省人才平台项目(贵州省平台人才[2020]2102)

Effect of low-salt salted duck egg pickling on free amino acids and flavor in yolk

  • HE Chunxia ,
  • WANG Xiujun ,
  • YU Pei ,
  • BAO Huanhuan ,
  • XU Jiuhong ,
  • LI Jiamin ,
  • FENG Tingcui
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  • 1(School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China)
    2(Guizhou Provincial Key Laboratory of Fermentation Engineering and Bio-pharmacy, Guiyang 550025, China)
    3(Zhenyuan County Mingcheng Food Factory, Qiandongnan Miao and Dong Autonomous Prefecture 557700, China)

Received date: 2022-08-04

  Revised date: 2022-11-12

  Online published: 2023-08-31

摘要

为探究三穗特色低盐咸鸭蛋在腌制过程中氨基酸种类、含量的变化,采用氨基酸分析仪对不同腌制时间的低盐咸鸭蛋黄游离氨基酸组成及含量进行测定,并采用主成分分析法探究游离氨基酸在咸蛋黄滋味中的呈味特性。结果表明,不同腌制时间检测到的游离氨基酸种类相同(16种),含有7种必需氨基酸,不同腌制时间游离氨基酸总含量有显著性差异,28 d腌制结束的咸蛋黄总游离氨基酸含量相较于0 d提高了46.49%,蛋白质相对含量提高了1.23倍。鲜味氨基酸、甜味氨基酸、苦味氨基酸、芳香族氨基酸含量均显著增加,0~7 d是呈味氨基酸含量增加的重要阶段。结合主成分分析和聚类分析结果得出脯氨酸、苯丙氨酸、酪氨酸、丙氨酸、谷氨酸、天冬氨酸、精氨酸、异亮氨酸8种氨基酸对咸蛋黄滋味影响程度最大。此为咸鸭蛋加工技术的改进及咸鸭蛋风味的人工控制提供理论基础。

本文引用格式

何春霞 , 王修俊 , 于沛 , 包欢欢 , 许九红 , 李佳敏 , 冯廷萃 . 低盐咸鸭蛋腌制过程对蛋黄中游离氨基酸和风味的影响[J]. 食品与发酵工业, 2023 , 49(15) : 243 -249 . DOI: 10.13995/j.cnki.11-1802/ts.033200

Abstract

To explore the changes in amino acid types and contents of Sansui low-salt salted duck eggs during pickling, an amino acid analyzer was used to determine the composition and content of free amino acids in low-salt salted duck egg yolk with different pickling time, and principal component analysis was used to evaluate the flavor characteristics of salted duck egg yolk. Results showed that 16 kinds of free amino acids were detected at different pickling times, containing seven kinds of essential amino acids. The total content of free amino acids had significant difference. The total content of free amino acids in salted egg yolk after 28 d of pickling increased by 46.49% compared with that at day 0, and the relative content of protein increased by 1.23 times. The contents of fresh amino acids, sweet amino acids, bitter amino acids, and aromatic amino acids increased significantly. The important stage for the increase of flavor amino acids was 0-7 d. Combined with the results of principal component analysis and cluster analysis, it was found that the eight amino acids of proline, phenylalanine, tyrosine, alanine, glutamic acid, aspartic acid, arginine, and isoleucine had the greatest influence on the taste of salted egg yolk. This provides a theoretical basis for the improvement of salted duck egg processing technology and the artificial control of salted duck egg flavor.

参考文献

[1] 李辉, 欧秀勇, 唐益成, 等.贵州省2个地方鸭品种蛋品质研究[J].江苏农业科学, 2010, 38(5):286-287.
LI H, OU X Y, TANG Y C, et al.Study on egg quality of two local duck breeds in Guizhou Province[J].Jiangsu Agricultural Sciences, 2010, 38(5):286-287.
[2] 沈畅萱, 王修俊, 黄珊.贵州三穗特色麻鸭蛋成分分析及营养评价[J].食品与机械, 2017, 33(12):55-60.
SHEN C X, WANG X J, HUANG S.The analysis and evaluation of nutrients of Sansui's characteristic sheldrake duck eggs[J].Food and Machinery, 2017, 33(12):55-60.
[3] 于沛, 王修俊, 刘林新, 等.不同三穗鸭蛋的挥发性风味成分比较分析[J].现代食品科技, 2021, 37(1):216-222;242.
YU P, WANG X J, LIU L X, et al.Comparative analysis of volatile flavor components of different Sansui duck eggs[J].Modern Food Science and Technology, 2021, 37(1):216-222;242.
[4] 顾永江, 陶宇航, 何明才, 等.三穗鸭品种特性概述及产业发展现状与对策[J].上海畜牧兽医通讯, 2013(6):44-45.
GU Y J, TAO Y H, HE M C, et al.Overview of variety characteristics of Sansui duck and its industrial development status and countermeasures[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine, 2013(6):44-45.
[5] 于沛, 王修俊, 徐雯, 等.贵州三穗特色黄皮蛋加工过程中凝胶形成及颜色变化分析[J].食品科学, 2021, 42(19):65-73.
YU P, WANG X J, XU W, et al.Analysis of gel formation and color change during the processing of yellow preserved eggs in Sansui, Guizhou[J].Food Science, 2021, 42(19):65-73.
[6] 王一亭, 庞敏.咸鸭蛋腌制机理与方法的研究[J].现代食品, 2020(22):52-54.
WANG Y T, PANG M.Study on salting mechanism and method of salted duck eggs[J].Modern Food, 2020(22):52-54.
[7] 罗雅. 咸蛋黄成网红 甜咸口味渐流行[J].中国食品, 2019(11):62-63.
LUO Y. Salted egg yolk is becoming more and more popular in online celebrity[J]. China Food, 2019(11):62-63.
[8] 王兆燃, 李文钊, 王强, 等.鸭蛋蛋黄加工过程中主要成分的变化[J].中国调味品, 2018, 43(2):1-3.
WANG Z R, LI W Z, WANG Q, et al.Changes in the main ingredients during processing of duck egg yolk[J].Chinese Condiment, 2018, 43(2):1-3.
[9] 陈远哲, 黄熙莺, 鲁安娜, 等.耐盐菌发酵对咸鸭蛋蛋清脱盐及抗氧化活性的影响[J].食品与生物技术学报, 2019, 38(11):130-136.
CHEN Y Z, HUANG X Y, LU A N, et al.Research on the desalination salted duck egg white and antioxidant activity by salt-tolerant bacterium fermentation[J].Journal of Food Science and Biotechnology, 2019, 38(11):130-136.
[10] 昝沛清, 程裕东, 金银哲.咸鸭蛋快速腌制工艺及咸蛋清综合利用研究进展[J].食品与机械, 2020, 36(10):210-214.
ZAN P Q, CHENG Y D, JIN Y Z.Research progress on rapid pickling process of salted duck eggs and comprehensive utilization of salted duck eggs white[J].Food and Machinery, 2020, 36(10):210-214.
[11] 邵萍. 酸浸减压法腌制咸蛋及其蛋白和脂质变化研究[D].天津:天津科技大学, 2017.
SHAO P.The research of acid soaking pretreatment and reduced pressure vacuum of salted egg and the change in protein and lipid during the processing[D].Tianjin:Tianjin University of Science and Technology, 2017.
[12] 欧阳玲花, 杜金平, 何家林, 等.负压快速腌制咸蛋研究[J].食品科技, 2015, 40(9):38-40.
OUYANG L H, DU J P, HE J L, et al.Research on quickly salted eggs by negative pressure technology[J].Food Science and Technology, 2015, 40(9):38-40.
[13] 杨哪, 金亚美, 徐悦, 等.磁电辅助快速腌制咸鸭蛋的工艺[J].农业工程学报, 2015, 31(21):295-300.
YANG N, JIN Y M, XU Y, et al.Processing of rapid salting on duck eggs by using magnetoelectric-assisted method[J].Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(21):295-300.
[14] 李德洪. 磁电辅助快速腌制咸鸭蛋的工艺分析[J].电子测试, 2016(17):175;172.
LI D H.Analysis of the process of quick curing Salted Duck Egg the magnetoelectric auxiliary[J].Electronic Test, 2016(17):175;172.
[15] 龙门, 张文豪, 郑素玲, 等.基于低场核磁共振技术的咸鸭蛋腌制过程水分及质构特性变化[J].食品与机械, 2019, 35(2):21-26;106.
LONG M, ZHANG W H, ZHENG S L, et al.Study on changes of gel moisture characteristic and texture properties of duck eggs during salting period by low-field nuclear magnetic resonance[J].Food and Machinery, 2019, 35(2):21-26;106.
[16] 孙秀秀, 何立超, 杨海燕, 等.间歇超声辅助加快咸蛋腌制速度工艺优化[J].食品工业科技, 2018, 39(22):204-211.
SUN X X, HE L C, YANG H Y, et al.Intermittent ultrasound assisted in speeding up the pickling speed of salted eggs[J].Science and Technology of Food Industry, 2018, 39(22):204-211.
[17] 范娟娟. 超声波辅助快速腌制咸蛋工艺的研究[D].福州:福建农林大学, 2013.
FAN J J.Study on the process of ultrasonic-assisted pickling salted egg[D].Fuzhou:Fujian Agriculture and Forestry University, 2013.
[18] 王石泉, 王树才, 张益鹏, 等.超声波-脉动压联用快速腌制咸鸭蛋的工艺参数优化[J].农业工程学报, 2013, 29(23):286-292.
WANG S Q, WANG S C, ZHANG Y P, et al.Parameter optimization for quickly salted egg by using ultrasonic-pulsed pressure technology[J].Transactions of the Chinese Society of Agricultural Engineering, 2013, 29(23):286-292.
[19] 吴玲. 咸蛋低钠脉动压腌制新技术的研究[D].武汉:华中农业大学, 2011.
WU L.Study on new technology of salted egg preserved with mixed low-sodium and pulse pressure[D].Wuhan:Huazhong Agricultural University, 2011.
[20] 丁德龙, 王鹏宇.低钠脉动压力技术对咸鸭蛋腌制渗透压及品质的影响[J].食品科技, 2021, 46(9):91-98.
DING D L, WANG P Y.Effect of low-sodium pressure fluctuation technology on osmotic pressure and quality of salted duck eggs[J].Food Science and Technology, 2021, 46(9):91-98.
[21] 李学贤, 张雪, 童灵, 等.游离氨基酸改善作物风味品质综述[J].中国农业大学学报, 2022, 27(4):73-81.
LI X X, ZHANG X, TONG L, et al.Summary of free amino acids to improve crop flavor quality[J].Journal of China Agricultural University, 2022, 27(4):73-81.
[22] 张晓维, 郑萍, 高洁, 等.3种方法腌制咸蛋理化性质的对比[J].食品研究与开发, 2020, 41(12):108-116.
ZHANG X W, ZHENG P, GAO J, et al.Comparison of physicochemical properties of salted eggs by three pickling methods[J].Food Research and Development, 2020, 41(12):108-116.
[23] ARDÖ Y.Flavour formation by amino acid catabolism[J].Biotechnology Advances, 2006, 24(2):238-242.
[24] 孙静, 李开耀, 向俊, 等.泥腌、水腌咸蛋的理化指标和滋味品质差异分析[J].食品安全质量检测学报, 2021, 12(21):8342-8350.
SUN J, LI K Y, XIANG J, et al.Comparison of physical and chemical indexes and taste quality of salted egg in single mud-salted and water-salted methods[J].Journal of Food Safety and Quality, 2021, 12(21):8342-8350.
[25] 孙静, 杜金平, 向俊, 等.腌制前清洗消毒对咸蛋游离氨基酸的影响[J].食品安全质量检测学报, 2021, 12(15):6160-6168.
SUN J, DU J P, XIANG J, et al.Effect of washing and disinfection before pickling on free amino acids in salted eggs[J].Journal of Food Safety and Quality, 2021, 12(15):6160-6168.
[26] PANG T, ZHANG H T, WEN L L, et al.Quantitative analysis of a weak correlation between complicated data on the basis of principal component analysis[J].Journal of Analytical Methods in Chemistry, 2021, 2021:1-12.
[27] 顾晓敏, 童川, 韩延超, 等.不同品种莲藕游离氨基酸多样性分析[J].食品科学, 2022, 43(4):183-189.
GU X M, TONG C, HAN Y C, et al.Diversity of free amino acids among different lotus rhizomes[J].Food Science, 2022, 43(4):183-189.
[28] 范婷婷, 赵晓燕, 李晓贝, 等.人工栽培和野生羊肚菌游离氨基酸主成分分析及综合评价[J].食品科学, 2022, 43(6):295-302.
FAN T T, ZHAO X Y, LI X B, et al.Principal component analysis for comprehensive evaluation of free amino acids in cultivated and wild Morchella[J].Food Science, 2022, 43(6):295-302.
[29] 魏光强, 李子怡, 黄艾祥, 等.基于游离氨基酸、挥发性组分和感官评价的2种酸化技术加工乳饼的滋味特征差异分析[J].食品科学, 2021, 42(22):263-269.
WEI G Q, LI Z Y, HUANG A X, et al.Differential taste characteristics of milk cakes processed by two acidification methods revealed by free amino acids, volatile compounds and sensory evaluation[J].Food Science, 2021, 42(22):263-269.
[30] 于沛. 三穗特色咸蛋低盐腌制控制技术研究[D].贵阳:贵州大学, 2021.
YU P.Study on low-salt curing control technology of characteristic salted eggs in Sansui[D].Guiyang:Guizhou University, 2021.
[31] 余平莲. 咸蛋黄加工中风味物质的变化及其成因初探[D].天津:天津科技大学, 2017.
YU P L.Changes of flavor compounds in salted egg yolk processing and preliminary study on the causes[D].Tianjin:Tianjin University of Science and Technology, 2017.
[32] 任发政. 咸蛋的腌制机理及其加工方法[J].农产品加工, 2009(5):24-25.
REN F Z.Pickling mechanism and processing method of salted eggs[J].Farm Products Processing, 2009(5):24-25.
[33] 孔祥会, 郭春丽, 郭彦玲, 等.食盐对草鱼血清超氧化物歧化酶和溶菌酶活性的影响[J].水生态学杂志, 2008, 29(5):80-83.
KONG X H, GUO C L, GUO Y L, et al.Effects of sodium chloride on activities of superoxide dismutase and lysozyme in serum of grass carp(ctenpharyngodon idella)[J].Journal of Hydroecology, 2008, 29(5):80-83.
[34] 苟琳, 姚倩, 傅泱.氯化钠对马铃薯酪氨酸酶的抑制作用[J].食品科技, 2010, 35(5):32-35.
GOU L, YAO Q, FU Y.Inhibitory effect of sodium chloride on potato tyrosinase[J].Food Science and Technology, 2010, 35(5):32-35.
[35] 朱佳源, 郑淘, 肖嫩群, 等.食盐对小鼠肠道微生物、酶活及血常规的影响[J].食品与机械, 2018, 34(9):43-46.
ZHU J Y, ZHENG T, XIAO N Q, et al.Effect of salt on intestinal microorganism, enzyme activities and blood in mice[J].Food and Machinery, 2018, 34(9):43-46.
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