研究报告

干酪乳酪杆菌Zhang在酸和低温胁迫下“活的非可培养”态的诱导研究

  • 代利霞 ,
  • 马学波 ,
  • 王会莹 ,
  • 刘春芳 ,
  • 薄晓宇 ,
  • 包秋华
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  • (内蒙古农业大学,乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古 呼和浩特, 010018)
第一作者:硕士研究生(包秋华副研究员为通信作者,E-mail: nmgbqh@126.com)

收稿日期: 2022-10-24

  修回日期: 2022-11-17

  网络出版日期: 2023-09-12

基金资助

国家自然科学地区基金项目(31860434;31301516);内蒙古自然科学基金项目(2019MS03051)

Induction of VBNC state of Lacticaseibacillus casei Zhang under the stress of acid and low temperature

  • DAI Lixia ,
  • MA Xuebo ,
  • WANG Huiying ,
  • LIU Chunfang ,
  • BO Xiaoyu ,
  • BAO Qiuhua
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  • (Inner Mongolia Agricultural University, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China)

Received date: 2022-10-24

  Revised date: 2022-11-17

  Online published: 2023-09-12

摘要

为探究干酪乳酪杆菌Zhang(Lacticaseibacillus casei Zhang)在酸和低温胁迫下能否形成活的非可培养(viable but non-culturable,VBNC)状态。该实验将L.casei Zhang置于不同pH值(2.5、3、4、5、6)的MRS液体培养基中,在4 ℃条件下进行酸和低温胁迫,不同时间点取样,然后采用平板计数法、荧光显微镜和流式细胞术3种方法对胁迫后的菌体进行活性测定。实验结果显示,低温条件下L.casei Zhang在pH值为2.5、3、5、6胁迫下未获得VBNC态;L.casei Zhang在pH 4的MRS液体培养基低温胁迫120 d获得了VBNC态,革兰氏染色观察到VBNC态细胞长度变短变弯曲。该研究为L.casei Zhang VBNC态的机理研究提供了一定的参考依据。

本文引用格式

代利霞 , 马学波 , 王会莹 , 刘春芳 , 薄晓宇 , 包秋华 . 干酪乳酪杆菌Zhang在酸和低温胁迫下“活的非可培养”态的诱导研究[J]. 食品与发酵工业, 2023 , 49(16) : 83 -87 . DOI: 10.13995/j.cnki.11-1802/ts.034087

Abstract

To investigate whether Lacticaseibacillus casei Zhang can form viable but non-culturable state (VBNC) under acid and low temperature stress, L. casei Zhang was cultured in MRS liquid medium with different pH (2.5, 3, 4, 5, 6) at 4 ℃ for acid and low temperature stress. Samples were taken at different time points, and then the activity of the strains after stress was measured by plate counting, fluorescence microscopy and flow cytometry. The results showed that L. casei Zhang did not obtain VBNC state under pH of 2.5, 3, 5, and 6 at 4 ℃ low. It entered the VBNC state by low temperature stress in MRS liquid medium at pH 4 after 120 d. Gram staining showed that the length of VBNC cells became shorter and curved. This study provides some reference for the mechanism study of L. casei Zhang VBNC state.

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