食品与发酵工业 >

2023 , Vol. 49 >Issue 17: 53 - 61

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034349

研究报告

燕窝中结合唾液酸调节5种益生菌生长以及孕妇肠道菌群组成的研究

  • 鲁佩涵 ,
  • 吴剑荣 ,
  • 柳训才 ,
  • 华玉蓉 ,
  • 詹晓北 ,
  • 高敏杰 ,
  • 张洪涛
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  • 1(糖化学与生物技术教育部重点实验室(江南大学),江苏 无锡,214122)
    2(厦门燕之屋丝浓食品有限公司燕窝研究院,福建 厦门,361000)
    3(江南大学附属医院,江苏 无锡,214122)
第一作者:硕士研究生(吴剑荣副教授为通信作者,E-mail:kinowu@jiangnan.edu.cn)

收稿日期: 2022-11-18

  修回日期: 2022-12-23

  网络出版日期: 2023-09-27

基金资助

国家重点研发计划项目(2021YFC2101402)

收起

Regulation of bacteria growth of five probiotics and intestinal flora of pregnant women by bound sialic acid of edible bird’s nest

  • LU Peihan ,
  • WU Jianrong ,
  • LIU Xuncai ,
  • HUA Yurong ,
  • ZHAN Xiaobei ,
  • GAO Minjie ,
  • ZHANG Hongtao
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(Xiamen Yan Palace Seelong Food Co.Ltd.Bird’s Nest Institute, Xiamen 361000, China)
    3(Affiliated Hospital of Jiangnan University, Wuxi 214122, China)

Received date: 2022-11-18

  Revised date: 2022-12-23

  Online published: 2023-09-27

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摘要

膳食动物源黏蛋白存在结合态唾液酸聚糖,可能对人体营养健康产生影响。该文以一种富含唾液酸的黏蛋白—燕窝(edible bird’s nest, EBN)为研究对象,通过酸处理切除燕窝糖蛋白末端唾液酸(acidolysis bird’s nest, ABN),分别进行体外模拟消化,比较酸处理前后的燕窝及唾液酸单体在抗氧化活性方面的差异,以及对不同益生菌和孕妇粪菌在体外发酵的影响。结果表明,EBN比ABN和唾液酸具有更好的抗氧化活性。EBN以及唾液酸可不同程度改善乳杆菌和双歧杆菌菌株的生长,并增加短链脂肪酸的生成。同时,EBN可以显著促进阿克曼黏细菌的生长。在体外进行的健康女性和孕晚期孕妇粪菌发酵中,EBN在24 h内可显著增加乙酸、丙酸和丁酸的产量,其中丙酸的增加最为显著。同时,EBN还能够降低大肠杆菌-志贺氏菌属、肠杆菌属和克雷伯氏菌属等潜在致病菌的相对丰度,并增加潜在益生菌(如拟杆菌属、粪杆菌属和粪球菌属)的相对丰度。EBN在降低致病菌丰度和增加益生菌丰度具有比ABN更加显著的效果。因此,EBN中结合了唾液酸的完整性对其生物学功能起重要作用。

关键词： 燕窝; 唾液酸; 益生菌; 肠道菌群; 短链脂肪酸

本文引用格式

鲁佩涵 , 吴剑荣 , 柳训才 , 华玉蓉 , 詹晓北 , 高敏杰 , 张洪涛 . 燕窝中结合唾液酸调节5种益生菌生长以及孕妇肠道菌群组成的研究[J]. 食品与发酵工业, 2023 , 49(17) : 53 -61 . DOI: 10.13995/j.cnki.11-1802/ts.034349

Abstract

There are bound sialoglycans in dietary mucin from animals, which may have an impact on human nutrition and health. In this work, a sialic acid-rich mucin, edible bird’s nest (EBN) was chosen as the research target. Also, the terminal sialic acid of EBN glycoprotein was removed through acidic treatment, forming acidolysis bird’s nest (ABN) and subject to in vitro digestion separately. The effects of these two types of bird’s nest and N-acetylneuraminic acid on the growth of probiotics and the fecal bacteria of pregnant women in vitro were investigated. Compared with the ABN, EBN and N-Acetylneuraminic acid could improve the growth of Lactobacillus and Bifidobacterium and increase the production of short chain fatty acids. EBN can significantly promote the growth of Akkermansia muciniphila. EBN can also increase the production of acetic acid, propionic acid, and butyric acid within 24 h during the in vitro fermentation of fecal bacteria of healthy women and late pregnant women, especially for the increase of propionic acid. Meanwhile, EBN can also reduce the relative abundance of potential pathogenic bacteria such as Escherichia coli-Shigella, Enterobacter, and Klebsiella, and increase the relative abundance of potential probiotics such as Bacteroides, Faecalibacterium and Coprococuus. EBN had more remarkable effect than ABN in reducing the abundance of pathogenic bacteria and increasing the abundance of probiotics. Therefore, the integrity of bound sialic acid of EBN plays an important role in its biological function.

Key words： edible bird’s nest; sialic acid; probiotics; intestinal flora; short chain fatty acids

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