食品与发酵工业 >

2023 , Vol. 49 >Issue 17: 202 - 208

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035762

研究报告

正己烷与氯化钙介导法联产提取未破壁螺旋藻的藻蓝蛋白和油脂

  • 郭旭 ,
  • 魏登枭 ,
  • 钟彩荣 ,
  • 兰英 ,
  • 何勇锦 ,
  • 陈必链
展开
  • 1(福建师范大学 生命科学学院,福建 福州,350117)
    2(工业微生物教育部工程研究中心(福建师范大学),福建 福州,350117)
第一作者:硕士研究生(陈必链教授为通信作者,E-mail:chenbil@fjnu.edu.cn)

收稿日期: 2023-04-08

  修回日期: 2023-04-23

  网络出版日期: 2023-09-27

基金资助

国家自然科学基金资助项目(32172997);福建省教育厅重点项目(2022G02013);福建师范大学碳中和研究院开放项目(TZH-2022-03)

收起

A novel extraction method mediated with n-hexane and calcium chloride to concurrently extract phycocyanin and lipids from unbroken Spirulina platensis

  • GUO Xu ,
  • WEI Dengxiao ,
  • ZHONG Cairong ,
  • LAN Ying ,
  • HE Yongjin ,
  • CHEN Bilian
Expand
  • 1(College of Life Sciences, Fujian Normal University, Fuzhou 350117, China)
    2(Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350117, China)

Received date: 2023-04-08

  Revised date: 2023-04-23

  Online published: 2023-09-27

Fold

摘要

以未破壁螺旋藻藻粉为原料,研发一种由有机溶剂与盐介导的新提取方法,可同时提取螺旋藻的藻蓝蛋白和油脂。为了获得最大联产提取性能,优化正己烷与CaCl2介导法的工艺参数。实验结果表明,适合联产提取未破壁螺旋藻藻蓝蛋白和油脂的工艺条件为:CaCl2 30 g/L,正己烷与悬浮液的体积比2∶1,料液比1∶60(g∶mL),提取时间3 h。在优化的条件下,正己烷与CaCl2介导法的藻蓝蛋白和油脂的最大提取率分别为71.06%和26.19%。正己烷与CaCl2介导法不仅可联产提取螺旋藻藻蓝蛋白和油脂,还具有分离所提取的藻蓝蛋白和油脂的功能,为螺旋藻深加工提取生物活性物质提供重要的理论依据。

关键词： 螺旋藻; 藻蓝蛋白; 油脂; 提取; 分离

本文引用格式

郭旭 , 魏登枭 , 钟彩荣 , 兰英 , 何勇锦 , 陈必链 . 正己烷与氯化钙介导法联产提取未破壁螺旋藻的藻蓝蛋白和油脂[J]. 食品与发酵工业, 2023 , 49(17) : 202 -208 . DOI: 10.13995/j.cnki.11-1802/ts.035762

Abstract

In this study, a new extraction method mediated by organic solvent and salt was developed to simultaneously extract phycocyanin and lipids from the unbroken Spirulina platensis. To obtain the maximum co-production extraction performance, the process parameters of the developed extraction method with n-hexane and calcium chloride were optimized. After optimization, the optimal conditions to extract phycocyanin and oil from unbroken Spirulina platensis using the n-hexane and calcium chloride-mediated method were that calcium chloride concentration was 3%, the volume ratio of n-hexane to suspension was 2:1, the ratio of algal biomass to liquid was 1:60 (g:mL), and extraction time was 3 h. Under the optimized conditions, the maximum extraction rates of phycocyanin and oil by the exploited extraction method were 71.06% and 26.19%, respectively. Based on these obtained results, the novel developed extraction method not only enables the co-production extraction of phycocyanin and lipids from Spirulina platensis but also has the function to separate the extracted phycocyanin and lipids, which provides important practical details to extract the desired bioactive substances from Spirulina platensis through Spirulina-derived deep processing.

Key words： Spirulina biomass; phycocyanin; lipid; extraction; separation

参考文献

[1] MA H T, XIONG H Y, ZHU X L, et al.Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice[J].International Journal of Biological Macromolecules, 2019, 133:1090-1101.
[2] 魏艳丽, 姜国庆, 彭坚, 等.螺旋藻的营养健康功能及在食品中应用研究进展[J].食品工业科技, 2022, 43(8):406-415.
WEI Y L, JIANG G Q, PENG J, et al.A review of nutrition and health functions of Spirulina and its application in food industry[J].Science and Technology of Food Industry, 2022, 43(8):406-415.
[3] YU J F.Application of an ultrafine shearing method for the extraction of C-phycocyanin from Spirulina platensis[J].Molecules, 2017, 22(11):2023.
[4] HADIYANTO H, SUTTRISNORHADI S.Response surface optimization of ultrasound assisted extraction (UAE) of phycocyanin from microalgae Spirulina platensis[J].Emirates Journal of Food and Agriculture, 2016, 28(4):227.
[5] KÄFERBÖCK A, SMETANA S, DE VOS R, et al.Sustainable extraction of valuable components from Spirulina assisted by pulsed electric fields technology[J].Algal Research, 2020, 48:101914.
[6] RIZWANUL F I M, NORAINI M Y, MOFIJUR M, et al.Lipid extraction maximization and enzymatic synthesis of biodiesel from microalgae[J].Applied Sciences, 2020, 10(17):6103.
[7] DE SOUSA E S A, MOREIRA L M, DE MAGALHÃES W T, et al.Extraction of biomolecules from Spirulina platensis using non-conventional processes and harmless solvents[J].Journal of Environmental Chemical Engineering, 2017, 5(3):2101-2106.
[8] LI Y, ZHANG Z, PACIULLI M, et al.Extraction of phycocyanin-a natural blue colorant from dried spirulina biomass:Influence of processing parameters and extraction techniques[J].Journal of Food Science, 2020, 85(3):727-735.
[9] WANG F, YU X L, CUI Y, et al.Efficient extraction of phycobiliproteins from dry biomass of Spirulina platensis using sodium chloride as extraction enhancer[J].Food Chemistry, 2023, 406:135005.
[10] HE Y J, CHEN L J, ZHOU Y C, et al.Analysis and model delineation of marine microalgae growth and lipid accumulation in flat-plate photobioreactor[J].Biochemical Engineering Journal, 2016, 111:108-116.
[11] LIAO N B, ZHONG J J, YE X Q, et al.Ultrasonic-assisted enzymatic extraction of polysaccharide from Corbicula fluminea:Characterization and antioxidant activity[J].LWT-Food Science and Technology, 2015, 60(2):1113-1121.
[12] MINKOVA K M, TCHERNOV A A, TCHORBADJIEVA M I, et al.Purification of c-phycocyanin from Spirulina (Arthrospira) fusiformis[J].Journal of Biotechnology, 2003, 102(1):55-59.
[13] CHAIKLAHAN R, CHIRASUWAN N, LOHA V, et al.Separation and purification of phycocyanin from Spirulina sp.using a membrane process[J].Bioresource Technology, 2011, 102(14):7159-7164.
[14] 魏登枭, 车丹丹, 陈必链, 等.三相分离法提取裂殖壶藻中油脂、蛋白质和多糖的工艺优化[J].中国油脂, 2023, 48(3):123-129.
WEI D X, CHE D D, CHEN B L, et al.Process optimization of three phase partitioning to extract the lipids, protein and polysaccharide from Schizochytrium sp[J].China Oils and Fats, 2023, 48(3):123-129.
[15] LAUCERI R, CAVONE C, ZITTELLI G C, et al.High purity grade phycocyanin recovery by decupling cell lysis from the pigment extraction:An innovative approach[J].Food and Bioprocess Technology, 2023, 16(1):111-121.
[16] 姜国庆, 闫秋丽, 李东, 等.螺旋藻中藻蓝蛋白提取、纯化及稳态化研究进展[J].食品安全质量检测学报, 2021, 12(6):2332-2338.
JIANG G Q, YAN Q L, LI D, et al.Research progress on separation, purification and stabilization of phycocyanin from Spirulina[J].Journal of Food Safety & Quality, 2021, 12(6):2332-2338.
[17] I·LTER I, AKYIL S, DEMIREL Z, et al.Optimization of phycocyanin extraction from Spirulina platensis using different techniques[J].Journal of Food Composition and Analysis, 2018, 70:78-88.
[18] WANG L L, WANG Y B, QIN Y C, et al.Extraction and determination of protein from edible oil using aqueous biphasic systems of ionic liquids and salts[J].Food and Bioprocess Technology, 2022, 15(1):190-202.
[19] HUANG Y F, ZHANG D M, XUE S Z, et al.The potential of microalgae lipids for edible oil production[J].Applied Biochemistry and Biotechnology, 2016, 180(3):438-451.
[20] 余佳, 赵岚, 张瑞华, 等.葛仙米藻胆蛋白的提取工艺优化及其纯化研究[J].食品研究与开发, 2018, 39(2):82-89.
YU J, ZHAO L, ZHANG R H, et al.Research on optimization of phycobiliprotein extraction from Nostoc sphaeroids Kutzing and the purification of phycobiliprotein[J].Food Research and Development, 2018, 39(2):82-89.
[21] PINTO L F R, FERREIRA G F, BEATRIZ F P, et al.Lipid and phycocyanin extractions from Spirulina and economic assessment[J].The Journal of Supercritical Fluids, 2022, 184:105567.
[22] YANG X H, LI Y, LI Y H, et al.Solid matrix-supported supercritical CO2 enhances extraction of γ-linolenic acid from the cyanobacterium Arthrospira (Spirulina) platensis and bioactivity evaluation of the molecule in zebrafish[J].Marine Drugs, 2019, 17(4):203.
[23] TAVANANDI H A, MITTAL R, CHANDRASEKHAR J, et al.Simple and efficient method for extraction of C-phycocyanin from dry biomass of Arthospira platensis[J].Algal Research, 2018, 31:239-251.
[24] 沈向阳, 梁霄, 付云, 等.钝顶螺旋藻藻蓝蛋白的提取工艺研究[J].现代食品科技, 2019, 35(7):198-204;136.
SHEN X Y, LIANG X, FU Y, et al.Study of the extraction process of phycocyanin from Spirulina platensis[J].Modern Food Science and Technology, 2019, 35(7):198-204;136.
[25] AYEKPAM C, HAMSAVI G K,.Efficient extraction of food grade natural blue colorant from dry biomass of Spirulina platensis using eco-friendly methods[J].Food and Bioproducts Processing, 2021, 129:84-93.
[26] LARROSA A P Q, CAMARA Á S, MOURA J M, et al.Spirulina sp.biomass dried/disrupted by different methods and their application in biofilms production[J].Food Science and Biotechnology, 2018, 27(6):1659-1665.
[27] OLIVEIRA E G, DUARTE J H, MORAES K, et al.Optimisation of Spirulina platensis convective drying:Evaluation of phycocyanin loss and lipid oxidation[J].International Journal of Food Science & Technology, 2010, 45(8):1572-1578.
[28] CRAMPON C, NIKITINE C, ZAIER M, et al.Oil extraction from enriched Spirulina platensis microalgae using supercritical carbon dioxide[J].The Journal of Supercritical Fluids, 2017, 119:289-296.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司