研究报告

凡纳滨对虾热风干制过程中内源性三甲胺的形成规律研究

  • 唐振冬 ,
  • 吉宏武 ,
  • 张迪 ,
  • 刘书成 ,
  • 苏伟明 ,
  • 宋文奎
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东 湛江,524088)
    2(海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁 大连,116034)
第一作者:硕士研究生(吉宏武教授和张迪讲师为共同通信作者,E-mail:Jihw62318@163.com;zjs578180838@sina.com)

收稿日期: 2022-08-13

  修回日期: 2022-09-08

  网络出版日期: 2023-09-27

基金资助

国家自然科学基金项目(32072340)国家虾蟹产业技术体系项目(CARs-48)

Study on formation regularity of endogenous trimethylamine during hot-air-drying of shrimp (Litopenaeus vannamei)

  • TANG Zhendong ,
  • JI Hongwu ,
  • ZHANG Di ,
  • LIU Shucheng ,
  • SU Weiming ,
  • SONG Wenkui
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University,Dalian 116034, China)

Received date: 2022-08-13

  Revised date: 2022-09-08

  Online published: 2023-09-27

摘要

该研究以凡纳滨对虾为原料,置于不同热风干制温度干制至水分含量约为20%,分别测定三甲胺(trimethylamine, TMA)、氧化三甲胺(trimetlylamine oxide, TMAO)、二甲胺(dimethylamine, DMA)和甲醛(formaldehyde, FA)等指标的含量变化。进一步探究了对虾在85 ℃下热风干制过程的水分含量、挥发性盐基氮(total volatile base nitrogen,TVB-N)、总还原力值、pH值、TMA、TMAO、FA和DMA等指标的变化情况。同时,为验证虾肉上清液中TMA的形成规律与整体的相似性,对不同反应时间、温度和pH条件下虾肉上清液中TMAO分解产生TMA的反应特性进行研究。结果表明,热风干制温度越高,TMAO通过非酶热分解形成的内源性TMA、DMA和FA含量越大。随着干制时间延长,对虾水分含量的降低,TVB-N值、总还原力和pH值增大,TMAO含量呈现显著下降趋势,内源性TMA、DMA和FA含量在干制后期显著上升,内源性TMA从开始的29.36 mg/kg显著上升至565.11 mg/kg (P<0.01)。虾肉上清液中包含了产生TMA的物质,同时其TMA的形成与热风干制过程虾干TMA的形成规律相一致,温度越高,加热时间越长,TMA产生越多。研究结果揭示了凡纳滨对虾在热风干制中内源性TMA的形成规律和产生机制,为虾干制品的加工和生产提供理论依据,以期为控制虾干TMA含量提供参考。

本文引用格式

唐振冬 , 吉宏武 , 张迪 , 刘书成 , 苏伟明 , 宋文奎 . 凡纳滨对虾热风干制过程中内源性三甲胺的形成规律研究[J]. 食品与发酵工业, 2023 , 49(17) : 223 -229 . DOI: 10.13995/j.cnki.11-1802/ts.033319

Abstract

In this study, Litopenaeus vannamei was used as raw material and dried at different hot air-drying temperatures until the moisture content was about 20%. The content changes of trimethylamine (TMA), trimethylamine oxide (TMAO), dimethylamine (DMA), and formaldehyde (FA) were measured respectively. The changes in moisture content, total volatile base nitrogen (TVB-N), total reducing power value, pH value, TMA, TMAO, FA, and DMA during hot-air-drying of shrimp at 85 ℃ were further explored. At the same time, the reaction characteristics of TMAO decomposition in shrimp meat supernatant under different reaction time, temperature, and pH conditions to produce TMA were studied to verify the similarity of the formation rule and the whole of TMA in shrimp meat supernatant. With increasing hot-air-drying temperature, non-enzymatic thermal decomposition of TMAO in white shrimp was observed, leading to a significant increase in the levels of endogenous TMA, DMA, and FA. Moreover, with the increase in drying time, the moisture content of shrimp decreased, TVB-N value, total reducing power, and pH value increased, TMAO content showed a significant downward trend, and endogenous TMA, DMA, and FA content increased significantly in the later drying period. Endogenous TMA increased significantly from the initial 29.36 mg/kg to 565.11 mg/kg (P<0.01). The supernatant of shrimp meat contains substances that produce TMA, and the formation of TMA was consistent with the formation of TMA in dried shrimp during hot air drying. The higher the temperature and the longer the heating time, the more TMA was produced. The formation law of endogenous TMA in the hot-air-drying process of shrimp provides a theoretical basis for the processing and production of dried shrimp products, to provide a basic reference for controlling the content of TMA in dried shrimp.

参考文献

[1] LI D Y, YUAN Z, LIU Z Q, et al.Effect of oxidation and Maillard reaction on color deterioration of ready-to-eat shrimps during storage[J].LWT, 2020, 131:109696.
[2] WU N, GU S Q, TAO N P, et al.Characterization of important odorants in steamed male Chinese mitten crab (Eriocheir sinensis) using gas chromatography-mass spectrometry-olfactometry[J].Journal of Food Science, 2014, 79(7):C1250-C1259.
[3] ZHANG D, JI H W, LIU S C, et al.Similarity of aroma attributes in hot-air-dried shrimp (Penaeus vannamei) and its different parts using sensory analysis and GC-MS[J].Food Research International, 2020, 137:109517.
[4] HU M Y, WANG S Y, LIU Q, et al.Flavor profile of dried shrimp at different processing stages[J].LWT, 2021, 146:111403.
[5] LIANG R, LIN S Y, CHEN D, et al.Differentiation of Penaeus vannamei from different thermal processing methods in physico-chemical, flavor and sensory characteristics[J].Food Chemistry, 2022, 378:132092.
[6] 朱军莉, 励建荣.鱿鱼及其制品加工贮存过程中甲醛的消长规律研究[J].食品科学, 2010, 31(5):14-17.
ZHU J L, LI J R.Change trend of formaldehyde in squid muscle and products during processing and storage[J].Food Science, 2010, 31(5):14-17.
[7] PANKYAMMA V, MOKAM S Y, DEBBARMA J, et al.Effects of microwave vacuum drying and conventional drying methods on the physicochemical and microstructural properties of squid shreds[J].Journal of the Science of Food and Agriculture, 2019, 99(13):5778-5783.
[8] BEKHIT A E D A, GITERU S G, HOLMAN B W B, et al.Total volatile basic nitrogen and trimethylamine in muscle foods:Potential formation pathways and effects on human health[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(4):3620-3666.
[9] HE S Y, JIANG H, ZHUO C L, et al.Trimethylamine/trimethylamine-N-oxide as a key between diet and cardiovascular diseases[J].Cardiovascular Toxicology, 2021, 21(8):593-604.
[10] ZHUANG K J, WU N, WANG X C, et al.Effects of 3 feeding modes on the volatile and nonvolatile compounds in the edible tissues of female Chinese mitten crab (Eriocheir sinensis)[J].Journal of Food Science, 2016, 81(4):S968-S981.
[11] CHEN Z Q, ZHU Y H, CAO W H, et al.Novel insight into the role of processing stages in nutritional components changes and characteristic flavors formation of noble scallop Chlamys nobilis adductors[J].Food Chemistry, 2022, 378:132049.
[12] 郭芮, 苏红, 张晓梅, 等.南美白对虾中氧化三甲胺热分解产甲醛的研究[J].核农学报, 2017, 31(11):2186-2193.
GUO R, SU H, ZHANG X M, et al.Research for the trimethylamine oxide thermally decompose into formaldehyde in Penaeus vannamei[J].Journal of Nuclear Agricultural Sciences, 2017, 31(11):2186-2193.
[13] LI Y C, DU F X, SONG S Z, et al.Effects of phenolic compounds from blueberry leaves on the thermal decomposition of trimethylamine oxide in squid extract[J].International Journal of Food Engineering, 2021, 17(4):285-297.
[14] 张泽伟, 董春雨, 吉宏武, 等.基于层次分析法优化凡纳滨对虾(Penaeus vannamei)热风干燥工艺[J].食品与发酵工业,2023,49(15):193-200.
ZHANG Z W, DONG C Y, JI H W, et al.Optimisation of the hot-air drying process for shrimp (Penaeus vannamei) based on analytic hierarchy process[J].Food and Fermentation Industries,2023,49(15):193-200.
[15] DONG L L, ZHU J L, LI X P, et al.Effect of tea polyphenols on the physical and chemical characteristics of dried-seasoned squid (Dosidicus gigas) during storage[J].Food Control, 2013, 31(2):586-592.
[16] 李颖畅, 李双燕, 曹娜娜, 等.赖氨酸-半乳糖对TMAO-Fe(Ⅱ)体系中TMAO热分解的影响[J].食品科学, 2021, 42(2):30-35.
LI Y C, LI S Y, CAO N N, et al.Effect of lysine-galactose mixture on the degradation of trimethylamine N-oxide in TMAO-Fe(Ⅱ) system[J].Food Science, 2021, 42(2):30-35.
[17] 朱军莉, 励建荣, 苗林林, 等.基于高温非酶途径的秘鲁鱿鱼内源性甲醛的控制[J].水产学报, 2010, 34(3):375-381.
ZHU J L, LI J R, MIAO L L, et al.Control on the intrinisic formaldehyde content in jumbo squid based on the non-enzymatic thermal pathway[J].Journal of Fisheries of China, 2010, 34(3):375-381.
[18] 靳肖, 周德庆, 孙永.鱿鱼丝氧化三甲胺热分解模拟体系的研究[J].食品工业科技, 2011, 32(3):106-108.
JIN X, ZHOU D Q, SUN Y.Study on thermal decomposition of trimethylamine-N-oxide model systems of shredded squid[J].Science and Technology of Food Industry, 2011, 32(3):106-108.
[19] ZHANG T, XIN X Q, XUE Y, et al.Reduction of formaldehyde residues induced by the thermal decomposition of trimethylamine oxide during the processing and storage of jumbo squid (Dosidicus gigas)[J].LWT, 2018, 97:676-683.
[20] 陈帅, 朱军莉, 潘伟春.乳糖对鱿鱼中氧化三甲胺热分解反应动力学的影响[J].现代食品科技, 2017, 33(3):116-121;145.
CHEN S, ZHU J L, PAN W C.Effects of galactose on the reaction kinetics of thermal degradation of TMAO in squid[J].Modern Food Science and Technology, 2017, 33(3):116-121;145.
[21] KIM S H, JUNG E J, HONG D L, et al.Quality assessment and acceptability of whiteleg shrimp (Litopenaeus vannamei) using biochemical parameters[J].Fisheries and Aquatic Sciences, 2020, 23:21.
[22] 周绪霞, 赵丹丹, 吕飞, 等.丁基羟基茴香醚处理对加速氧化期间毛虾干制品品质的影响[J].食品工业科技, 2013, 34(24):344-346.
ZHOU X X, ZHAO D D, LYU F, et al.Effect of BHA on the quality of dried product of Acetes chinensis during accelerated oxidation process[J].Science and Technology of Food Industry, 2013, 34(24):344-346.
[23] ZHU J L, WU S S, WANG Y H, et al.Quality changes and browning developments during storage of dried-seasoned squid (Dosidicus gigas and Ommastrephes bartrami)[J].Journal of Aquatic Food Product Technology, 2016, 25(7):1107-1119.
[24] WANG Y X, HUANG H Y, SHI W Z.Effect of different drying time on physicochemical properties of black carp (Mylopharyngodon piceus) by hot air[J].Journal of Food Processing and Preservation, 2022, 46(2):e16217.
[25] 张旭飞, 罗晓琳, 吉宏武, 等.凡纳滨对虾水煮加热过程颜色与虾青素及体外抗氧化活性的相关性[J].广东海洋大学学报, 2021, 41(3):105-112.
ZHANG X F, LUO X L, JI H W, et al.Correlation between astaxanthin coloration and in vitro antioxidant activity of Litopenaeus vannamei during boiling and heating[J].Journal of Guangdong Ocean University, 2021, 41(3):105-112.
[26] ZHU J L, JIA J A, LI X P, et al.ESR studies on the thermal decomposition of trimethylamine oxide to formaldehyde and dimethylamine in jumbo squid (Dosidicus gigas) extract[J].Food Chemistry, 2013, 141(4):3881-3888.
[27] 李颖畅, 杜凤霞, 张笑, 等.蓝莓叶多酚对鱿鱼上清液中氧化三甲胺高温热分解的影响[J].中国食品学报, 2020, 20(8):183-190.
LI Y C, DU F X, ZHANG X, et al.Effects of polyphenols extracted from blueberry leaves on the thermal decomposition of trimethylamine oxide in squid supernatant[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(8):183-190.
[28] 杨美竹, 王慧森, 刘璇, 等.热处理对鱿鱼上清液中氧化三甲胺降解的影响[J].中国调味品, 2022, 47(1):55-59.
YANG M Z, WANG H S, LIU X, et al.Effect of heat treatment on TMAO degradation in squid supernatant[J].China Condiment, 2022, 47(1):55-59.
[29] DENG Y, LIU Y M, QIAN B J, et al.Impact of far-infrared radiation-assisted heat pump drying on chemical compositions and physical properties of squid (Illex illecebrosus) fillets[J].European Food Research and Technology, 2011, 232(5):761-768.
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