食品与发酵工业 >

2023 , Vol. 49 >Issue 18: 41 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032907

研究报告

天山冷水藻油多不饱和脂肪酸纯化及其体外抗氧化活性研究

  • 艾合买提江·艾海提 ,
  • 艾斯卡尔·吐尔逊 ,
  • 古丽菲热·伊力哈木 ,
  • 肖移聪 ,
  • 秦新政 ,
  • 艾买提·买买提明 ,
  • 祖丽胡玛尔·亚库普 ,
  • 茹先古丽·买买提依明 ,
  • 刘军
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  • 1(新疆大学 生命科学与技术学院,新疆 乌鲁木齐,830046)
    2(新疆农业科学院微生物应用研究所,新疆 乌鲁木齐,830000)
    3(新疆新光生物科技有限公司,新疆 乌鲁木齐,830000)
第一作者:硕士,副教授(茹先古丽·买买提依明讲师和刘军副教授为共同通信作者,E-mail:725276492@qq.com;liujunxju@sina.com)

收稿日期: 2022-07-07

  修回日期: 2022-08-01

  网络出版日期: 2023-10-25

基金资助

国家重点研发计划(2021YFD1100603)

收起

Purification of polyunsaturated fatty acids of Tianshan cold water algae oil and study on its antioxidant activity in vitro

  • AIHAITI Aihemaitijiang ,
  • TUERXUN Aisikaer ,
  • YILIHAMU Gulifeire ,
  • XIAO Yicong ,
  • QIN Xinzheng ,
  • Aimaiti MAIMAITIMING ,
  • Zulihumaer YAKUPU ,
  • Ruxianguli MAIMAITIYIMING ,
  • LIU Jun
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  • 1(School of Life Science and Technology, Xinjiang University, Urumqi 830046, China)
    2(Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China)
    3(Xinjiang Xinguang Biotechnology Co.Ltd., Urumqi 830000, China)

Received date: 2022-07-07

  Revised date: 2022-08-01

  Online published: 2023-10-25

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摘要

为确定天山冷水藻油多不饱和脂肪酸(Tianshan cold water algae oil polyunsaturated fatty acids,TAOP)的纯化工艺条件,测定天山冷水藻粉(Tianshan cold water algae powder,TAP)、天山冷水藻油(Tianshan cold water algae oil,TAO)以及TAOP活性成分、测定其对体外抗氧化能力的影响,进行相关性分析,该研究以TAP为原料,利用尿素包合法对TAO进行工艺优化,测定TAP和TAOP中的活性成分含量,并对总抗氧化能力、DPPH自由基清除能力、超氧阴离子自由基清除能力、ABTS阳离子自由基清除能力之间进行相关性分析。结果表明,TAOP工艺优化最佳条件为:脂肪酸尿素质量比1∶3.98,结晶温度10.71 ℃,包合时间16.31 h,回流时间27.67 min,在此条件下,综合评价值为132.74。TAP和TAOP的抗氧化能力随着多酚、三萜、黄酮、总酸等成分含量的降低而下降,并且其中多酚、三萜、黄酮、总酸等成分含量与抗氧化活性具有正相关关系(P<0.05)。研究结果可为TAOP纯化工艺提供简便的新的优化方法,通过研究TAP、TAO、TAOP的抗氧化活性为其开发及应用提供科学依据和参考基础。

关键词： 冷水藻; 藻油; 多不饱和脂肪酸; 纯化; 抗氧化; 相关性分析

本文引用格式

艾合买提江·艾海提 , 艾斯卡尔·吐尔逊 , 古丽菲热·伊力哈木 , 肖移聪 , 秦新政 , 艾买提·买买提明 , 祖丽胡玛尔·亚库普 , 茹先古丽·买买提依明 , 刘军 . 天山冷水藻油多不饱和脂肪酸纯化及其体外抗氧化活性研究[J]. 食品与发酵工业, 2023 , 49(18) : 41 -49 . DOI: 10.13995/j.cnki.11-1802/ts.032907

Abstract

To determine the purification process conditions of Tianshan cold water algae oil polyunsaturated fatty acids (TAOP), Tianshan cold water algae powder (TAP), Tianshan cold water algae oil (TAO) and active components of TAOP, and their effects on antioxidant capacity in vitro and correlation analysis, TAP was used as raw material to optimize the process of TAO by urea inclusion method. The contents of active components in TAP and TAOP were determined, and the correlation analysis was conducted on the total antioxidant capacity, DPPH radical scavenging capacity, superoxide anion radical scavenging capacity, and ABTS cationic radical scavenging capacity. Results showed that the optimum conditions of the TAOP process were that the mass ratio of fatty acid to urea was 1∶3.98, crystallization temperature was 10.71 ℃, inclusion time was 16.31 h, and reflux time was 27.67 min. Under these conditions, the comprehensive evaluation value was 132.74. The antioxidant capacity of TAP and TAOP decreased with the decrease of the content of polyphenols, triterpenes, flavonoids, total acids, and other components. The content of polyphenols, triterpenes, flavonoids, total acids, and other components presented a positive correlation with the antioxidant activity (P<0.05). TAOP purification process provides a new and simple optimization method. The research results could provide a simple and new optimization method for the purification process of TAOP. By studying the antioxidant activities of TAP, TAO and TAOP, it provides a scientific basis and certain data reference basis for its development and application.

Key words： cold water algae; algal oil; polyunsaturated fatty acids; purification; antioxidant; correlation analysis

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