研究报告

超声预处理对鸡骨香精基料美拉德反应及生物活性的影响

  • 王亮 ,
  • 田玉潭 ,
  • 刘军 ,
  • 郑安然 ,
  • 毛筱艺 ,
  • 吴迅 ,
  • 刘敦华
展开
  • 1(宁夏大学 食品与葡萄酒学院,宁夏 银川,750021)
    2(宁夏大学 农学院,宁夏 银川,750021)
第一作者:硕士研究生(刘敦华教授为通信作者,E-mail:dunhualiu@126.com)

收稿日期: 2022-06-07

  修回日期: 2022-07-25

  网络出版日期: 2023-10-25

基金资助

国家星火计划(2015GA880005);宁夏自然科学基金(2021AAC03013;2022AAC02021);宁夏回族自治区农业科技自主创新资金(NGSB-2021-6-05)

Effect of ultrasound pretreatment on Maillard reaction and bioactive properties of chicken bone essence basic material

  • WANG Liang ,
  • TIAN Yutan ,
  • LIU Jun ,
  • ZHENG Anran ,
  • MAO Xiaoyi ,
  • WU Xun ,
  • LIU Dunhua
Expand
  • 1(School of Food and Wine, Ningxia University, Yinchuan 750021, China)
    2(School of Agriculture, Ningxia University, Yinchuan 750021, China)

Received date: 2022-06-07

  Revised date: 2022-07-25

  Online published: 2023-10-25

摘要

为充分开发鸡骨的潜在利用价值,该研究以鸡骨为原料,利用酶解发酵技术联合美拉德反应制备鸡骨香精基料,分析热处理、超声协同热处理制备鸡骨香精基料的美拉德反应体系,并探究鸡骨香精基料的生物活性。结果表明,超声预处理后,鸡骨香精基料美拉德反应的中间产物、褐变程度、荧光强度显著增加(P<0.05),主要呈味物质醛类、烷烃类、杂环类化合物相对含量增加,表明超声能够促进鸡骨香精基料美拉德反应的发生,改善鸡骨香精基料的风味;鸡骨香精基料的DPPH自由基、ABTS阳离子自由基、超氧阴离子自由基清除率最高分别为95.45%、83.82%、90.63%,血管紧张素转换酶(angiotensin-converting enzyme,ACE)抑制率最高为90.00%,体外降胆固醇能力最高为30.97%,证明鸡骨香精基料具备一定的抗氧化能力、ACE抑制能力和体外降胆固醇能力。该实验明确超声预处理对鸡骨香精基料美拉德反应程度及风味的影响,为开发具有抗氧化活性和抑制活性的高品质肉味衍生化产品提供一定参考。

本文引用格式

王亮 , 田玉潭 , 刘军 , 郑安然 , 毛筱艺 , 吴迅 , 刘敦华 . 超声预处理对鸡骨香精基料美拉德反应及生物活性的影响[J]. 食品与发酵工业, 2023 , 49(18) : 97 -104 . DOI: 10.13995/j.cnki.11-1802/ts.032596

Abstract

To exploit the potential utilization value of chicken bone, this study used chicken bone as raw material to prepare the essence basic material using enzymatic hydrolysis and fermentation technology combined with Maillard reaction, analyzed the system of Maillard reaction of chicken bone essence basis material prepared by heat treatment and ultrasonic synergistic heat treatment, as well as explored the biological activity of it. Results showed that the intermediate products, browning degree, and fluorescence intensity of the Maillard reaction of chicken bone essence basis material significantly increased (P<0.05) after ultrasonic pretreatment, and the relative contents of the main flavor-presenting substances aldehydes, alkanes, and heterocyclic compounds increased, indicating that ultrasound could promote the occurrence of the Maillard reaction of chicken bone essence basic material and improve the flavor while the highest scavenging rates of DPPH free radicals, ABTS cationic radicals, and superoxide anion radicals were 95.45%, 83.82%, and 90.63%, respectively, and the highest angiotensin-converting enzyme (ACE) inhibition rate was 90.00%, the highest in vitro cholesterol-lowering ability was 30.97%, which proved that the chicken bone essence basis material had the definite antioxidant ability, ACE inhibition, and cholesterol inhibition. This study demonstrated the effect of ultrasonic pretreatment on the degree of Maillard reaction and flavor of chicken bone essence basis material, providing a reference for the development of high-quality meat flavor derivatization products with antioxidant and inhibitory activity.

参考文献

[1] MIRABELLA N, CASTELLANI V, SALA S.Current options for the valorization of food manufacturing waste:A review[J].Journal of Cleaner Production, 2014, 65:28-41.
[2] DONG Z Y, LI M Y, TIAN G, et al.Effects of ultrasonic pretreatment on the structure and functionality of chicken bone protein prepared by enzymatic method[J].Food Chemistry, 2019, 299:125103.
[3] KONG Y, YANG X, DING Q, et al.Comparison of non-volatile umami components in chicken soup and chicken enzymatic hydrolysate[J].Food Research International, 2017, 102:559-566.
[4] 陈树俊, 徐晓霞, 胡洁, 等.苦荞、燕麦和杏鲍菇酶解液发酵工艺优化及其抗氧化活性[J].食品工业科技, 2018, 39(3):153-157;166.
CHEN S J, XU X X, HU J, et al.Optimization of fermentation process of enzymatic hydrolysate of buckwheat, oat and Pleurotus eryngii and its antioxidant capacity[J].Science and Technology of Food Industry, 2018, 39(3):153-157;166.
[5] YANG X, LI Y L, LI S T, et al.Effects and mechanism of ultrasound pretreatment of protein on the Maillard reaction of protein-hydrolysate from grass carp (Ctenopharyngodon idella)[J].Ultrasonics Sonochemistry, 2020, 64:104964.
[6] SUN H M, WANG J Z, ZHANG C H, et al.Changes of flavor compounds of hydrolyzed chicken bone extracts during Maillard reaction[J].Journal of Food Science, 2014, 79(12):C2415-C2426.
[7] HABINSHUTI I, MU T H, ZHANG M.Structural, antioxidant, aroma, and sensory characteristics of Maillard reaction products from sweet potato protein hydrolysates as influenced by different ultrasound-assisted enzymatic treatments[J].Food Chemistry, 2021, 361:130090.
[8] 田玉潭. 鸡骨热反应香精基料的制备及抗氧化与抑制性的研究[D].银川:宁夏大学, 2021.
TIAN Y T.Study on preparation and antioxidant and inhibitoryity of chicken bone thermal reaction flavor base[D].Yinchuan:Ningxia University, 2021.
[9] 刘伟. 超声波处理对河蚌肉酶解物美拉德反应的影响及其微囊化调味料制备[D].长春:吉林大学, 2019.
LIU W.Effect of ultrasonic treatment on Maillard reaction of hydrolysate derived from mussel meat and its manufacture of microencapsulated seasoning[D].Changchun:Jilin University, 2021.
[10] TAN J E, LIU T T, YAO Y, et al.Changes in physicochemical and antioxidant properties of egg white during the Maillard reaction induced by alkali[J].LWT, 2021, 143:111151.
[11] 依胜男, 芦晶, 逄晓阳, 等.热处理对牛乳美拉德反应程度及挥发性成分的影响[J].食品科学, 2021, 42(14):9-15.
YI S N, LU J, PANG X Y, et al.Effect of heat treatment on Maillard reaction degree and volatile components of milk[J].Food Science, 2021, 42(14):9-15.
[12] XIONG G Y, CHEN X, ZHANG X X, et al.Process optimization and the relationship between the reaction degree and the antioxidant activity of Maillard reaction products of chicken liver protein hydrolysates[J].Poultry Science, 2020, 99(7):3733-3741.
[13] 张亚琨, 张美莉, 郭新月.微粉碎对燕麦麸皮功能性成分及抗氧化性的影响[J].中国食品学报, 2021, 21(11):22-28.
ZHANG Y K, ZHANG M L, GUO X Y.Effect of micronization on the functional components and antioxidant properties in oat bran[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(11):22-28.
[14] LI X Y, FENG C S, HONG H, et al.Novel ACE inhibitory peptides derived from whey protein hydrolysates:Identification and molecular docking analysis[J].Food Bioscience, 2022, 48:101737.
[15] 李晶, 薛霖莉, 郝左亮, 等.冰葡果醋的功能成分及抗氧化、降血糖和降胆固醇作用[J].中国调味品, 2019, 44(5):197-200.
LI J, XUE L L, HAO Z L, et al.Functional components, antioxidation, hypoglycemic and cholesterol-lowering effects of ice grape vinegar[J].China Condiment, 2019, 44(5):197-200.
[16] XU B G, ZHANG M, BHANDARI B, et al.Effect of ultrasound immersion freezing on the quality attributes and water distributions of wrapped red radish[J].Food and Bioprocess Technology, 2015, 8(6):1366-1376.
[17] ZHENG Z L, ZHANG M, LIU W C, et al.Effect of beef tallow, phospholipid and microwave combined ultrasonic pretreatment on Maillard reaction of bovine bone enzymatic hydrolysate[J].Food Chemistry, 2021, 377:131902.
[18] 陈晓. 鸡肝蛋白酶解物的美拉德反应条件优化及其产物特性[D].合肥:安徽农业大学, 2020.
CHEN X.Optimization of Maillard reaction conditions for chicken liver protein enzymatic hydrolysate and its product characteristics[D].Hefei:Anhui Agricultural University, 2020.
[19] LI Q, LI X J, REN Z Y, et al.Physicochemical properties and antioxidant activity of Maillard reaction products derived from Dioscorea opposita polysaccharides[J].LWT, 2021, 149:111833.
[20] SUN W Z, ZHAO M M, CUI C, et al.Effect of Maillard reaction products derived from the hydrolysate of mechanically deboned chicken residue on the antioxidant, textural and sensory properties of Cantonese sausages[J].Meat Science, 2010, 86(2):276-282.
[21] BAEK H H, CADWALLADER K R.Enzymatic hydrolysis of crayfish processing by-products[J].Journal of Food Science, 1995, 60(5):929-935.
[22] ZHAN P, TIAN H L, ZHANG X M, et al.Contribution to aroma characteristics of mutton process flavor from the enzymatic hydrolysate of sheep bone protein assessed by descriptive sensory analysis and gas chromatography olfactometry[J].Journal of Chromatography B Analyt Technol Biomed Life, 2013, 921-922(6):1-8.
[23] 刘子轩, 高雅, 王文倩, 等.不同品种食用菌制备热反应肉味基料风味差异分析[J].食品科学技术学报, 2022, 40(1):30-43.
LIU Z X, GAO Y, WANG W Q, et al.Flavor difference analysis of thermal processing meat flavoring prepared with different varieties of mushroom materials[J].Journal of Food Science and Technology, 2022, 40(1):30-43.
[24] BI S, LAO F, PAN X, et al.Flavor formation and regulation of peas (Pisum sativum L.) seed milk via enzyme activity inhibition and off-flavor compounds control release[J].Food Chemistry, 2022, 380:132203.
[25] WANG H Y, QIAN H, YAO W R.Melanoidins produced by the Maillard reaction:Structure and biological activity[J].Food Chemistry, 2011, 128(3):573-584.
[26] RUFIÁN-HENARES J A, MORALES F J.Angiotensin-i converting enzyme inhibitory activity of coffee melanoidins[J].Journal of Agricultural and Food Chemistry, 2007, 55(4):1480-1485.
[27] LI F J, OHNISHI-KAMEYAMA M, TAKAHASHI Y, et al.Angiotensin I-converting enzyme inhibitory activities of Chinese fermented soypaste and estimation of the inhibitory substances[J].Journal of Functional Foods, 2013, 5(4):1991-1995.
[28] 刘兰涛. 山西老陈醋醋泥的降血脂和抗氧化活性研究[D].太原:山西大学, 2016.
LIU L T.Research on hypolipidemic and antioxidant activity of Shanxi aged vinegar sludge[D].Taiyuan:Shanxi university, 2016.
[29] PAK V V, KOO M, LEE N, et al.Structure—Activity relationships of the peptide Ile-Ala-Val-pro and its derivatives revealed using the semi-empirical am1 method[J].Chemistry of Natural Compounds, 2005, 41(4):454-460.
[30] OH N S, KWON H S, LEE H A, et al.Preventive effect of fermented Maillard reaction products from milk proteins in cardiovascular health[J].Journal of Dairy Science, 2014, 97(6):3300-3313.
文章导航

/