食品与发酵工业 >

2023 , Vol. 49 >Issue 18: 105 - 112

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032761

研究报告

六种柚果不同部位矿物质元素、总酚、柚皮苷含量测定及抗氧化活性研究

  • 张声源 ,
  • 陈炜铃 ,
  • 王楠 ,
  • 古秋明 ,
  • 况伟 ,
  • 陈嘉乐 ,
  • 苏文欣 ,
  • 刘志伟
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  • 1(嘉应学院,广东省山区特色农业资源保护与精准利用重点实验室,广东 梅州,514015)
    2(梅州市南方金柚研究院,广东 梅州,518000)
    3(嘉应学院广东省柚类工程技术开发中心,广东 梅州,514031)
第一作者:博士,副教授(刘志伟教授为通信作者,E-mail:zhweiliu@vip.sina.com)

收稿日期: 2022-06-22

  修回日期: 2022-08-04

  网络出版日期: 2023-10-25

基金资助

广东省企业科技特派员项目(GDKTP2020068500);梅州市应用型科技专项资金项目(2020B0205004;2021B0201001);广东省自然科学基金项目(2016A030307011);梅州市医药卫生科研课题(2020-B-79);广东省大学生创新创业项目(S201910582108)

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Study on mineral elements, total polyphenol, naringin content, and antioxidant activity in different parts of six pomelo cultivars

  • ZHANG Shengyuan ,
  • CHEN Weiling ,
  • WANG Nan ,
  • GU Qiuming ,
  • KUANG Wei ,
  • CHEN Jiale ,
  • SU Wenxin ,
  • LIU Zhiwei
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  • 1(Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou 514015, China)
    2(Meizhou Institute of Southern Golden Pomelo, Meizhou 518000, China)
    3(Guangdong Pomelo Engineering Technology Development Center, Meizhou 514031, China)

Received date: 2022-06-22

  Revised date: 2022-08-04

  Online published: 2023-10-25

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摘要

比较研究6种柚果(沙田柚、四季柚、黄肉蜜柚、红肉蜜柚、白肉蜜柚、三红蜜柚)的外皮层、中皮层、囊衣、汁胞4个部位的矿物质元素、总酚、柚皮苷含量及抗氧化活性差异。采用福林酚比色法、高效液相法、火焰原子吸收光谱法分别测定总酚、柚皮苷、矿物质元素含量;以DPPH法、ABTS法和普鲁士蓝法分别测定6种柚子4个部位95%(体积分数)乙醇提取物的自由基清除能力和铁离子还原能力。结果表明,矿物质元素中Mg和Ca元素含量较高,Mg元素最高可达(25 349.50±1.03) μg/g DW(三红蜜柚外皮层)、Ca元素最高可达(14 704.50±1.08) μg/g DW(三红蜜柚外皮层);黄肉蜜柚外皮层总酚含量最高,达(6.14±0.31) mg GA/g DW;黄肉蜜柚中皮层柚皮苷含量最高,达(11.95±0.06) mg/g DW。综合抗氧化活性指数表明,外皮层抗氧化活性最强的品种是黄肉蜜柚,其他部位抗氧化活性最强的品种均是四季柚。相关性分析显示,总酚、柚皮苷的含量与ABTS阳离子自由基清除能力均呈显著性正相关(P<0.01)。该研究可为柚类资源的开发利用及品质评价提供参考依据。

关键词： 柚子; 总酚; 柚皮苷; 抗氧化; 矿物质元素

本文引用格式

张声源 , 陈炜铃 , 王楠 , 古秋明 , 况伟 , 陈嘉乐 , 苏文欣 , 刘志伟 . 六种柚果不同部位矿物质元素、总酚、柚皮苷含量测定及抗氧化活性研究[J]. 食品与发酵工业, 2023 , 49(18) : 105 -112 . DOI: 10.13995/j.cnki.11-1802/ts.032761

Abstract

The contents of mineral elements, total polyphenol, naringin, and antioxidant activities from different parts (flavedo, mediopellis, segment membrane, juice sac) of 6 pomelo cultivars [Citrus maxima (Burm.) Merr. cv. Shatian yu, Citrus grandis (Linn) Osbeckl. cv. Siji yu, Citrus maxima (Burm.) Merr.cv.Bairoumi yu, Citrus maxima (Burm.) Merr.cv.Hongroumi yu, Citrus maxima (Burm.) Merr.cv.Sanhongmi yu, and Citrus maxima (Burm.) Merr.cv.Huangroumi yu] were researched by comparative study. The contents of total polyphenol, naringin, and mineral elements were determined by Folin-Ciocalteu colorimetric, HPLC method, flame atomic absorption spectrometry, and so on. Antioxidant activities of 95% ethanol extracts from different parts of pomelo were evaluated by methods of DPPH radical scavenging, ABTS cationic radical scavenging, and Prussian blue. Results showed that the mineral elements content of calcium [(25 349.50±1.03) μg/g] and magnesium [(14 704.50±1.08) μg/g] were highest in flavedo parts of Citrus maxima (Burm.) Merr.cv.Sanhongmi yu. The part with the highest content of total phenols was in the flavedo of Citrus maxima (Burm.) Merr.cv.Hongroumi yu, (6.14±0.31) mg GA/g DW, and the part with the highest content of naringin was in the mediopellis of Citrus maxima (Burm.) Merr.cv.Hongroumi yu, (11.95±0.06) mg/g DW. The antioxidant potency composite (APC) index showed that the highest antioxidant activity of flavedo was in Citrus maxima (Burm.) Merr.cv.Hongroumi yu and the highest antioxidant activity of the other parts were both in Citrus grandis (Linn) Osbeckl. cv. Siji yu. ABTS cationic radical scavenging activity had an extremely significant positive correlation with the content of total polyphenol and naringin (P<0.01). This research provides a reference for the reasonable development and utilization of pomelo resources and quality evaluation systems.

Key words： pomelo; total polyphenol; naringin; antioxidant; mineral elements

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