母乳是婴儿营养物质的主要来源且蕴藏着丰富的乳酸菌资源。为了了解新疆地区母乳中乳酸菌组成,筛选母乳中具有优良益生特性的双歧杆菌。该文通过传统纯培养方法对6份新疆地区母乳中的乳酸菌进行分离鉴定。以益生菌株Bifidobacterium lactis BB12作为对照,通过发酵特性、体外胃肠液耐受性及胆盐耐受性试验对双歧杆菌进行筛选,得到2株具有潜在益生特性的双歧杆菌,对其进行最适pH,最适温度,对NaCl的耐受性,生长曲线及菌株产酸能力等生理生化特性试验。从6份样品中共分离到69株乳酸菌,鉴定为7个属11个种及亚种,其中Streptococcus salivarius为优势菌,通过初步筛选获得2株具有潜在益生特性的双歧杆菌:Bifidobacterium longum IMAU99727和Bifidobacterium animalis subsp. lactis IMAU99728,有望作为新的母乳源益生菌进行其他益生特性研究和开发利用。
Breast milk is the main source of nutrients for infants and is rich in lactic acid bacteria. In order to understand the composition of lactic acid bacteria in breast milk in Xinjiang, bifidobacteria with excellent probiotic properties were screened for breast milk. In this paper, lactic acid bacteria in six parts of breast milk in Xinjiang were isolated and identified by traditional pure culture method. The probiotic Bifidobacterium lactis BB12 was used as a control, and Bifidobacterium was screened by fermentation characteristics, in vitro gastrointestinal fluid tolerance and bile salt tolerance tests. Two Bifidobacterium strains with potential probiotic properties were obtained. The physiological and biochemical characteristics such as optimum pH, optimum temperature, tolerance to NaCl, growth curve and acid production ability of the strains were tested. A total of 69 strains of lactic acid bacteria were isolated from 6 samples and identified as 11 species and subspecies of 7 genera, of which Streptococcus salivarius was the dominant bacteria. Two strains of Bifidobacterium with potential probiotic properties were obtained by preliminary screening: Bifidobacterium longum IMAU99727 and Bifidobacterium animalis subsp. lactis IMAU99728, which are expected to be used as new breast milk probiotics for other probiotic properties research and development and utilization.
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