食品与发酵工业 >

2023 , Vol. 49 >Issue 19: 190 - 198

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033018

研究报告

海黄牡丹不同部位的成分分析、体外抗氧化能力及其α-葡萄糖苷酶、α-淀粉酶抑制能力

  • 代嫚婷 ,
  • 宋静 ,
  • 余潇 ,
  • 张平芳 ,
  • 平怀磊 ,
  • 刘子榕 ,
  • 王娟 ,
  • 王振兴
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  • 1(西南林业大学 园林园艺学院,云南 昆明,650224)
    2(西南林业大学 林学院,云南 昆明,650224)
    3(西南林业大学 绿色发展研究院,云南 昆明,650224)
    4(西南林业大学 生命科学学院,云南 昆明,650224)
第一作者:硕士研究生(王娟教授与王振兴讲师为共同通信作者,E-mail:schima@163.com;wangzhenxingfood@163.com)

收稿日期: 2022-07-18

  修回日期: 2022-08-19

  网络出版日期: 2023-11-01

基金资助

云南省重大基础专项生物资源数字化开发应用项目(202002AA10007);国家自然科学基金项目(32060089);云南省万人计划“云岭产业技术领军人才”专项(云发改[2018]212号);云南省千人计划专项(2019);云南省中央引导地方科技发展资金项目(202207AB110015)

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The phytochemical profiling, antioxidant capacity, α-glucosidase and α-amylase inhibition abilities of different parts of Paeonia suffruticosa ‘Hai Huang'

  • DAI Manting ,
  • SONG Jing ,
  • YU Xiao ,
  • ZHANG Pingfang ,
  • PING Huailei ,
  • LIU Zirong ,
  • WANG Juan ,
  • WANG Zhenxing
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  • 1(College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China)
    2(College of Forestry, Southwest Forestry University, Kunming 650224, China)
    3(Eco-development Academy, Southwest Forestry University, Kunming 650224, China)
    4(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)

Received date: 2022-07-18

  Revised date: 2022-08-19

  Online published: 2023-11-01

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摘要

为比较海黄牡丹的花瓣、花萼、雌蕊、雄蕊4个部位的化学成分和功能活性,测定其总酚、总黄酮含量,采用HPLC进一步分析5种多酚含量,并评估其抗氧化能力、α-葡萄糖苷酶和α-淀粉酶活性抑制能力。结果表明,海黄牡丹4个部位的总酚含量介于347.72～917.82 mg GAE/g,总黄酮含量介于13.21～58.71 mg RT/g,其中雌蕊、花萼的总酚和总黄酮含量显著高于其他2个部位(P<0.05)。HPLC分析发现不同部位之间化合物成分含量差异显著;在定量的5个多酚化合物中,花瓣部位检测到的多酚种类最多,雄蕊部位芹菜素和异鼠李素含量最高(P<0.05)。此外,花萼部位有较强的DPPH自由基和ABTS阳离子自由基清除能力,其IC50值分别为44.97和74.44 μg/mL,雌蕊部位的铁还原能力最强为691.46 mg FeSO4/g。花瓣对α-葡萄糖苷酶有较好的抑制作用,而雄蕊则对α-淀粉酶的抑制能力最强,优于阳性对照阿卡波糖。综上结果,海黄牡丹的雌蕊和花萼部位具有较好的抗氧化能力,而花瓣和雄蕊部位具有一定的体外降血糖的能力。该研究为海黄牡丹开发为天然抗氧化、降血糖食品提供一定的科学依据。

关键词： 海黄牡丹; 活性成分; 抗氧化能力; α-葡萄糖苷酶; α-淀粉酶

本文引用格式

代嫚婷 , 宋静 , 余潇 , 张平芳 , 平怀磊 , 刘子榕 , 王娟 , 王振兴 . 海黄牡丹不同部位的成分分析、体外抗氧化能力及其α-葡萄糖苷酶、α-淀粉酶抑制能力[J]. 食品与发酵工业, 2023 , 49(19) : 190 -198 . DOI: 10.13995/j.cnki.11-1802/ts.033018

Abstract

To compare the phytochemical profiling and functional activity of petals, calyx, pistil, and stamen of Paeonia suffruticosa ‘Hai Huang'. The total phenolic (TPC) and total flavonoid (TFC) contents of these four parts were quantified, and their 5 polyphenols contents were further analyzed by HPLC. Finally, the antioxidant ability, α-glucosidase and α-amylase inhibition abilities of these four parts were evaluated. Results showed that the TPC of these four parts ranged between 347.72 to 917.82 mg GAE/g, while TFC ranged between 13.21 to 58.71 mg RT/g. Of these, pistil and calyx showed significantly higher TPC and TFC than those of the other two parts (P<0.05). From HPLC results, remarkable differences in their bioactive compound contents were observed between different parts of the samples (P<0.05). Among them, the petal detected the most compounds frequently, and the stamen detected the highest compounds contents. In addition, the calyx displayed the strongest DPPH radical and ABTS radical with IC50 values of 44.97 and 74.44 μg/mL, respectively. Pistil had the best ferric capacity, and its value was 691.46 mg FeSO4/g. In these four parts, the petal showed strong inhibition of α-glucosidase, while stamen displayed a superior inhibitory effect against α-amylase, which was better than the positive control acarbose. In summary, the pistil and calyx parts of Hai Huang exhibited superior antioxidant activity, while petal and stamen showed certain in vitro hypoglycemic activity. This study can provide some scientific basis for developing Hai Huang into natural antioxidant and hypoglycemic.

Key words： Paeonia suffruticosa ‘Hai Huang'; bioactive constituents; antioxidant capacity; α-glucosidase; α-amylase

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