食品与发酵工业 >

2023 , Vol. 49 >Issue 22: 19 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034949

研究报告

产细菌素乳酸菌的筛选及其益生性能评价

  • 张乐 ,
  • 丁一珍 ,
  • 潘媛娜 ,
  • 李科 ,
  • 耿燕 ,
  • 周娟 ,
  • 许泓瑜 ,
  • 李恒 ,
  • 许正宏 ,
  • 史劲松
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  • 1(江南大学 生命科学与健康工程学院,江苏 无锡,214112)
    2(粮食发酵与食品生物制造国家工程研究中心,江苏 无锡,214112)
第一作者：硕士研究生(李恒副教授为通信作者,E-mail:liheng@jiangnan.edu.cn)

收稿日期: 2023-01-27

  修回日期: 2023-03-06

  网络出版日期: 2023-12-25

基金资助

国家自然科学基金项目(22278181);宁夏回族自治区重点研发计划(2020BFH02011)

收起

Screening of bacteriocin-producing lactic acid bacteria and the probiotic properties evaluation

  • ZHANG Le ,
  • DING Yizhen ,
  • PAN Yuanna ,
  • LI Ke ,
  • GENG Yan ,
  • ZHOU Juan ,
  • XU Hongyu ,
  • LI Heng ,
  • XU Zhenghong ,
  • SHI Jinsong
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  • 1(College of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Research Center for Food Fermentation and Food Biomanufacturing, Wuxi 214122, China)

Received date: 2023-01-27

  Revised date: 2023-03-06

  Online published: 2023-12-25

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摘要

产细菌素乳酸菌可通过肠道微生态的调节发挥益生作用。研究从发酵食品中筛选到20株乳酸菌,通过排除有机酸和过氧化氢等干扰,获得一株抑菌能力较强的产细菌素的哈尔滨乳杆菌(Lactobacillus harbinensis)P1-1。对该菌株的肠道益生性能进行评价,并通过微生物共培养评价其与微生物之间的关系。结果显示,L.harbinensis P1-1在人工胃肠液中的存活率能达到90%以上,在1.2 g/L的胆盐中存活率高达56.2%,具有良好的胃肠道消化液耐受性。L.harbinensis P1-1能显著抑制条件致病菌大肠杆菌(Escherichia coli)ATCC 25922和表皮葡萄球菌(Staphylococcus epidermidis)ATCC 12228增殖,24 h抑菌率分别为86.3%和89.5%。采用共培养方法考察L.harbinensis P1-1的益生特性。L.harbinensis P1-1对益生菌L.plantarum CGMCC No.18389和E.coli Nissle 1917的生长均无明显影响,但可显著抑制2株条件致病菌的生长,存活率低于90%,同时益生菌与条件致病菌都可增强L.harbinensis P1-1的抑菌活性,表明L.harbinensis P1-1具有良好的肠道益生活性,具有进一步性能挖掘和应用拓展的潜力。

关键词： 乳酸菌; 细菌素; 抑菌; 共培养; 哈尔滨乳杆菌

本文引用格式

张乐 , 丁一珍 , 潘媛娜 , 李科 , 耿燕 , 周娟 , 许泓瑜 , 李恒 , 许正宏 , 史劲松 . 产细菌素乳酸菌的筛选及其益生性能评价[J]. 食品与发酵工业, 2023 , 49(22) : 19 -26 . DOI: 10.13995/j.cnki.11-1802/ts.034949

Abstract

Bacteriocin-producing lactic acid bacteria exhibit probiotic effects through regulating intestinal microecology. In the present study, a bacteriocin-producing strain of Lactobacillus harbinensis P1-1 with strong antibacterial ability was screened from 20 strains of lactic acid bacteria by eliminating the bacteriostasis effects of organic acid and hydrogen peroxide. The probiotic effects of L. harbinensis P1-1 were evaluated and the relationship between this strain and some other microorganisms were evaluated through microbial coculture. The results showed that the survival rate of L. harbinensis P1-1 in artificial gastrointestinal fluids could reach more than 90%, while the survival rate in 1.2 g/L bile salt was 56.2%. It indicated that this strain possessed good gastrointestinal digestive fluid tolerance. L. harbinensis P1-1 inhibited the proliferation of Escherichia coli ATCC 25922 and Staphylococcus epidermidis ATCC 12228 significantly with the inhibition rate reached 86.3% and 89.5% at 24 h, respectively. The co-culture method was utilized to investigate the probiotic properties of L. harbinensis P1-1. It showed L. harbinensis P1-1 exerted little effects on growth of the two probiotic L. plantarum CGMCC No.18389 and Escherichia coli Nissle 1917, while the growth of two conditional pathogens were both inhibited apparently with a survival rate of less than 90%. Meanwhile, the antibacterial activity of L. harbinensis P1-1 was enhanced by co-cultured with all these microorganisms, which represented that L. harbinensis P1-1 possessed positive intestinal prebiotic activity and had the potential for further exploration of its performance and application.

Key words： lactic acid bacteria; bacteriocin; bacteriostasis; co-culture; Lactobacillus harbinensis

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