食品与发酵工业 >

2024 , Vol. 50 >Issue 1: 163 - 169

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034819

研究报告

西藏凹乳芹多糖的分离纯化、结构表征及抗氧化活性研究

  • 赵泽素 ,
  • 南木加 ,
  • 刘双平 ,
  • 毛健
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  • 1(江南大学 生物工程学院,江苏 无锡,214122)
    2(粮食发酵与食品生物制造国家工程研究中心(江南大学),江苏 无锡,214122)
    3(西藏藏医药大学,基础部,西藏 拉萨,850000)
    4(江南大学(绍兴)产业技术研究院,浙江 绍兴,312000)
    5(国家黄酒工程技术研究中心,浙江 绍兴,312000)
第一作者:硕士研究生(毛健教授和南木加副教授为共同通信作者,E-mail:maojian@jiangnan.edu.cn;1796944976@qq.com)

收稿日期: 2023-01-05

  修回日期: 2023-02-09

  网络出版日期: 2024-01-31

基金资助

藏医药“十四五”规划内涵建设一期项目(2021ZYYGH008)

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Isolation, purification, structure characterization, and antioxidant activity of polysaccharides from Vicatia thibetica de Boiss

  • ZHAO Zesu ,
  • NAN Mujia ,
  • LIU Shuangping ,
  • MAO Jian
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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)
    3(Basic Department, University of Tibetan Medicine, Lhasa 850000, China)
    4(Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing 312000, China)
    5(National Engineering Research Center for Huangjiu, Shaoxing 312000, China)

Received date: 2023-01-05

  Revised date: 2023-02-09

  Online published: 2024-01-31

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摘要

该文以西藏凹乳芹为原料,超声辅助酶法提取多糖,经DEAE-52纤维素和Sephadex G-100 凝胶柱分离纯化,得到水溶性西藏凹乳芹多糖(Vicatia thibetica de Boiss polysaccharide,VTP)的纯化组分VTP-1。通过高效凝胶渗透色谱、离子色谱、紫外可见光谱、傅立叶红外光谱、刚果红等实验对其分子质量、单糖组成、空间结构、理化性质和体外抗氧化活性进行研究。结果表明,VTP-1相对分子质量为96.81 kDa,由阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖组成,物质的量之比为5.63∶2.79∶86.36∶3.02∶1.59。VTP-1是以α和β-型糖苷键共同连接的中性杂多糖且无三螺旋结构。VTP-1对DPPH自由基、ABTS阳离子自由基、·OH清除率的半抑制浓度值分别为1.808、1.845、4.634 mg/mL,抑制率均表现为依赖剂量性增加,表明其具有良好的抗氧化活性。综上,该实验对西藏凹乳芹多糖首次开展研究,证明了其体外抗氧化活性,为西藏凹乳芹多糖在功能性食品以及医药行业的应用提供了理论依据。

关键词： 西藏凹乳芹; 多糖; 纯化; 结构表征; 抗氧化活性

本文引用格式

赵泽素 , 南木加 , 刘双平 , 毛健 . 西藏凹乳芹多糖的分离纯化、结构表征及抗氧化活性研究[J]. 食品与发酵工业, 2024 , 50(1) : 163 -169 . DOI: 10.13995/j.cnki.11-1802/ts.034819

Abstract

A new polysaccharide was extracted from Vicatia thibetica de Boiss using an ultrasound-assisted enzymatic method. After separation and purification by DEAE-52 cellulose ion column chromatography and Sephadex G-100 glucan gel column chromatography, the water-soluble Vicatia thibetica de Boiss polysaccharide (VTP-1) was obtained. High-performance gel permeation chromatography (HPGPC), ion chromatography, UV-vis spectrum, Fourier transform infrared spectroscopy, and Congo red experiment were applied to analyze the molecular weight, monosaccharide composition, spatial structure, physicochemical properties, and antioxidant activity in vitro of polysaccharides. Results showed that the relative molecular weight of VTP-1 was 96.81 kDa. It was composed of arabinose, galactose, glucose, xylose, and mannose in the molar ratio of 5.63∶2.79∶86.36∶3.02∶1.59. VTP-1 was identified as a neutral heteropolysaccharide containing both α- and β-type glycosidic bonds and did not have a triple helix structure. The IC50 values of DPPH free radical, ABTS cationic radical, and hydroxyl radical scavenging ability were 1.808, 1.845, and 4.634 mg/mL, respectively, and the inhibition rates showed a dose-dependent increase, indicating that VTP-1 had good antioxidant activity in vitro. In conclusion, this experiment was the first to study the polysaccharide of Vicatia thibetica de Boiss, which proved its antioxidant activity in vitro. It could provide a theoretical basis for the application of Vicatia thibetica de Boiss polysaccharides in the functional food and medicine industry.

Key words： Vicatia thibetica de Boiss; polysaccharides; purification; structure characteristics; antioxidant activity

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