食品与发酵工业 >

2024 , Vol. 50 >Issue 2: 57 - 66

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033270

研究报告

兰州百合鳞茎花青素合成相关MYB与bHLH转录因子的筛选分析

  • 范文广 ,
  • 李保豫 ,
  • 田辉 ,
  • 李欣 ,
  • 任海伟 ,
  • 曹莹莹 ,
  • 姜欣彤 ,
  • 柴佳靖 ,
  • 陈少青
展开
  • (兰州理工大学 生命科学与工程学院,甘肃 兰州,730050)
第一作者:博士,副教授(通信作者,E-mail:fanwenguang_88@163.com)

收稿日期: 2022-08-09

  修回日期: 2022-10-26

  网络出版日期: 2024-02-27

基金资助

国家自然科学基金项目 (31960491);甘肃省科技计划项目(20JR10RA159)

收起

Screening and analysis of MYB and bHLH transcription factors associated with anthocyanin synthesis in Lilium davidii var. unicolor bulbs

  • FAN Wenguang ,
  • LI Baoyu ,
  • TIAN Hui ,
  • LI Xin ,
  • REN Haiwei ,
  • CAO Yingying ,
  • JIANG Xintong ,
  • CHAI Jiajing ,
  • CHEN Shaoqing
Expand
  • (School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China)

Received date: 2022-08-09

  Revised date: 2022-10-26

  Online published: 2024-02-27

Fold

摘要

百合鳞茎在采后贮藏过程中,由于花青素的积累导致鳞茎变紫红色,进而影响其价值。该文基于兰州百合鳞茎转录组数据,利用生物信息学技术分析花青素相关转录因子,共分析了50个MYB转录因子与73个bHLH转录因子,包括序列的比对、理化性质分析、亚细胞定位、系统进化关系以及保守结构域预测。MYB分类结果显示,50个MYB转录因子中有2个属于1R-MYB类,44个属于R2R3-MYB类,3个属于3R-MYB类,1个属于4R-MYB类。50个MYB不稳定蛋白均为亲水性蛋白,亚细胞定位表明48个MYB定位于细胞核。73个bHLH均为不稳定蛋白,且72个都为亲水性,亚细胞定位显示58个bHLH定位于细胞核。通过与模式植物拟南芥MYB和bHLH转录因子构建进化树分析、保守结构域预测、基因表达量分析以及转录因子与结构基因的相关性分析,初步筛选出3个MYB基因和1个bHLH基因可促进兰州百合鳞茎花青素合成,为进一步深入研究兰州百合鳞茎花青素调控基因提供了理论支持。

关键词： 兰州百合(Lilium davidii var.unicolor); 鳞茎; MYB转录因子; bHLH转录因子; 花青素合成

本文引用格式

范文广 , 李保豫 , 田辉 , 李欣 , 任海伟 , 曹莹莹 , 姜欣彤 , 柴佳靖 , 陈少青 . 兰州百合鳞茎花青素合成相关MYB与bHLH转录因子的筛选分析[J]. 食品与发酵工业, 2024 , 50(2) : 57 -66 . DOI: 10.13995/j.cnki.11-1802/ts.033270

Abstract

During the post-harvest storage of lily bulbs, the accumulation of anthocyanins causes the bulbs to turn violet-red, which in turn affects their value. Based on transcriptome data in Lilium davidii var. unicolor bulbs and analysis of anthocyanin-related transcription factors using bioinformatics technology, including sequence alignment, physicochemical property analysis, subcellular localization, phylogenetic relationship, and prediction of conserved domains, a total of 53 MYB transcription factors and 73 bHLH transcription factors were analyzed. The MYB classification results showed that 2 of them belonged to the 1R-MYB class, 44 of them belonged to the R2R3-MYB class, 3 of them belonged to the 3R-MYB class, and 1 of them belonged to the 4R-MYB class. Among 50 MYB unstable proteins showed hydrophilicity, and subcellular localization indicated that 48 MYBs were localized to the nucleus. All 73 bHLHs were unstable proteins, and 72 were hydrophilic. Subcellular localization showed that 58 bHLHs were localized in the nucleus. By constructing phylogenetic tree analysis with the model plant Arabidopsis MYB and bHLH transcription factors, the prediction of conserved domains, gene expression analysis, as well as correlation analysis between transcription factors and structural genes, 3 MYB genes and 1 bHLH genes that promote anthocyanin synthesis were preliminarily screened out from the bulbs of Lilium davidii var. unicolor. It provides theoretical support for further in-depth study of anthocyanin-regulated genes in Lilium davidii var. unicolor bulbs.

Key words： Lilium davidii var. unicolor; bulb; MYB transcription factor; bHLH transcription factor; anthocyanin synthesis

参考文献

[1] 陈俊愉. 中国花卉品种分类学[M].北京:中国林业出版社, 2001.
CHEN J Y.Taxonomy of Chinese Flower Varieties[M].Beijing:China Forestry Press, 2001.
[2] 夏婷, 耿兴敏, 罗凤霞.不同花色野生百合色素成分分析[J].东北林业大学学报, 2013, 41(5):109-113;166.
XIA T, GENG X M, LUO F X.Components of flower pigments in the petals of wild Lilium in China[J].Journal of Northeast Forestry University, 2013, 41(5):109-113;166.
[3] 王瑜, 崔金腾, 张克中, 等.百合花青素苷合成酶基因片段的克隆及表达分析[J].中国农学通报, 2013, 29(10):162-166.
WANG Y, CUI J T, ZHANG K Z, et al.Molecular cloning and expression analysis of anthocyanidin synthase gene fragment in Lilium[J].Chinese Agricultural Science Bulletin, 2013, 29(10):162-166.
[4] 胡悦, 杜运鹏, 田翠杰, 等.百合属植物化学成分及其生物活性的研究进展[J].食品科学, 2018, 39(15):323-332.
HU Y, DU Y P, TIAN C J, et al.A review of chemical components and their bioactivities from the genus Lilium[J].Food Science, 2018, 39(15):323-332.
[5] KANO M, TAKAYANAGI T, HARADA K, et al.Antioxidative activity of anthocyanins from purple sweet potato, Ipomoera batatas cultivar Ayamurasaki[J].Bioscience, Biotechnology, and Biochemistry, 2005, 69(5):979-988.
[6] 范国良, 刘养卉.兰州百合产业现状与研究进展[J].甘肃农业, 2013(4):12-13.
FAN G L, LIU Y H.Present situation and research progress of lily industry in Lanzhou[J].Gansu Agriculture, 2013(4):12-13.
[7] 肖伟. 兰州百合LdMYB6基因的克隆与初步功能分析[D].长春:吉林农业大学, 2019.
XIAO W.Cloning and preliminary functional analysis of LdMYB6 gene from Lilium davidii var. unicolor[D].Changchun:Jilin Agricultural University, 2019.
[8] NØRBAEK R, KONDO T.Anthocyanins from flowers of Lilium (Liliaceae)[J].Phytochemistry, 1999, 50(7):1181-1184.
[9] 陈洁, 安利清, 王涛, 等.百合查尔酮合成酶基因克隆及其转化烟草的花色表达分析[J].西北植物学报, 2012, 32(8):1511-1517.
CHEN J, AN L Q, WANG T, et al.Cloning of chalcone synthase gene in Lilium and expression analysis of flower colour changes in transgenic tobacco[J].Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(8):1511-1517.
[10] 覃仁娟. 百合DFR基因克隆及功能初步鉴定[D].雅安:四川农业大学, 2014.
QIN R J.Cloning and functional initial characterization of the gene encoding dihydroflavonol 4-reductase (DFR) from lily[D].Ya′an:Sichuan Agricultural University, 2014.
[11] 窦晓莹, 郎利新, 包放, 等.东方百合查尔酮异构酶基因LhCHI的克隆及表达[J].东北林业大学学报, 2015,43(9):6-11;17.
DOU X Y, LANG L X, BAO F, et al.Cloning and expression analysis of chalcone isomerase gene LhCHI in oriental hybrid lily (Lilium spp.)[J].Journal of Northeast Forestry University, 2015,43(9):6-11;17.
[12] YAMAGISHI M, SHIMOYAMADA Y, NAKATSUKA T, et al.Two R2R3-MYB genes, homologs of Petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of Asiatic hybrid lily[J].Plant and Cell Physiology, 2010, 51(3):463-474.
[13] YAMAGISHI M.A novel R2R3-MYB transcription factor regulates light-mediated floral and vegetative anthocyanin pigmentation patterns in Lilium regale[J].Molecular Breeding, 2016, 36(1):1-14.
[14] NAKATSUKA A, YAMAGISHI M, NAKANO M, et al.Light-induced expression of basic helix-loop-helix genes involved in anthocyanin biosynthesis in flowers and leaves of Asiatic hybrid lily[J].Scientia Horticulturae, 2009, 121(1):84-91.
[15] HUANG D J, LI W T, DAWUDA M M, et al.Hydrogen sulfide reduced colour change in Lanzhou lily-bulb scales[J].Postharvest Biology and Technology, 2021, 176:111520.
[16] GARG V K, AVASHTHI H, TIWARI A, et al.MFPPI-multi FASTA ProtParam interface[J].Bioinformation, 2016, 12(2):74-77.
[17] DUBOS C, STRACKE R, GROTEWOLD E, et al.MYB transcription factors in Arabidopsis[J].Trends in Plant Science, 2010, 15(10):573-581.
[18] THORLEY J L, PAGE R D M.RadCon:Phylogenetic tree comparison and consensus[J].Bioinformatics, 2000, 16(5):486-487.
[19] GONZALEZ A, ZHAO M Z, LEAVITT J M, et al.Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings[J].The Plant Journal, 2008, 53(5):814-827.
[20] KRANZ H D, DENEKAMP M, GRECO R, et al.Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana[J].The Plant Journal, 1998, 16(2):263-276.
[21] STRACKE R, WERBER M, WEISSHAAR B.The R2R3-MYB gene family in Arabidopsis thaliana[J].Current Opinion in Plant Biology, 2001, 4(5):447-456.
[22] 刘振, 武俊刚, 李响, 等.基于转录组数据的绢毛委陵菜MYB转录因子家族分析[J/OL].分子植物育种, 2021.http://kns.cnki.net/kcms/detail/46.1068.S.20210722.1457.024.html.
LIU Z, WU J G, LI X, et al.Identification of the MYB transcription factor family of Potentilla sericata and screening of the MYB gene regulating heavy metal Cadmium[J/OL].Molecular Plant Breeding, 2021.http://kns.cnki.net/kcms/detail/46.1068.S.20210722.1457.024.html.
[23] 谢冬冬, 王武萍, 何学高, 等.基于转录组的漆树MYB转录因子的筛选及分析[J].西北林学院学报, 2021, 36(1):108-116.
XIE D D, WANG W P, HE X G, et al.Screening and analysis of MYB transcription factors based on transcriptome data in Toxicodendron vernicifluum[J].Journal of Northwest Forestry University, 2021, 36(1):108-116.
[24] 王雪倩, 袁国振, 吴泽, 等.亚洲百合MYB转录因子家族的鉴定及调控花粉败育MYB基因的筛选[J].农业生物技术学报, 2019, 27(11):1951-1961.
WANG X Q, YUAN G Z, WU Z, et al.Identification of MYB transcription factors family and screening MYB genes associated with pollen abortion in Lilium Asiatic hybrids[J].Journal of Agricultural Biotechnology, 2019, 27(11):1951-1961.
[25] 冯磊, 石元豹, 汪贵斌, 等.银杏bHLH家族转录因子生物信息学及表达分析[J].江苏农业学报, 2019, 35(2):400-411.
FENG L, SHI Y B, WANG G B, et al.Bioinformatics and expression analysis of transcription factors of ginkgo bHLH family[J].Jiangsu Journal of Agricultural Sciences, 2019, 35(2):400-411.
[26] 刘静, 王翠平, 朱强, 等.黑果枸杞bHLH转录因子家族的生物信息学分析[J].分子植物育种, 2020, 18(14):4612-4623.
LIU J, WANG C P, ZHU Q, et al.Bioinformatics analysis of bHLH transcription factor family in Lycium ruthenicum Murr.[J].Molecular Plant Breeding, 2020, 18(14):4612-4623.
[27] HEIM M A, JAKOBY M, WERBER M, et al.The basic helix-loop-helix transcription factor family in plants:A genome-wide study of protein structure and functional diversity[J].Molecular Biology and Evolution, 2003, 20(5):735-747.
[28] SATO S, NAKAMURA Y, KANEKO T, et al.Structural analysis of Arabidopsis thaliana chromosome 3.I.sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones[J].DNA Research, 2000, 7(2):131-135.
[29] NESI N, DEBEAUJON I, JOND C, et al.The TT8 gene encodes a basic helix-loop-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques[J].The Plant Cell, 2000, 12(10):1863-1878.
[30] 孙颖琦, 孟亚轩, 赵心月, 等.谷子bHLH转录因子家族基因鉴定及生物信息学分析[J].种子, 2021, 40(12):45-55.
SUN Y Q, MENG Y X, ZHAO X Y, et al.Identification and bioinformatics analysis of transcription factor family genes bHLH in foxtail millet[J].Seed, 2021, 40(12):45-55.
[31] FAN W G, LI B Y, TIAN H, et al.Metabolome and transcriptome analysis predicts metabolism of violet-red color change in Lilium bulbs[J].Journal of the Science of Food and Agriculture, 2022, 102(7):2903-2915.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司