该文对从实验室筛选出的具有强耐镉能力的肉葡萄球菌(Staphylococcus carnosus)846进行了降镉特性和降镉机制的研究。分析了pH值、Cd2+浓度、菌体浓度和其他金属离子等因素对其降镉能力的影响,并通过扫描电镜,能谱和傅里叶光谱探究了该菌的降镉机制。结果表明,当pH值为5,Cd2+质量浓度为50 mg/L和菌体质量浓度为1 g/L时,菌体的降Cd2+效率最高;Mg2+、Pb2+和Zn2+等金属离子的存在会对菌体的降Cd2+效果产生负面影响,而Cr6+的存在提高了菌体的降Cd2+能力。Cd2+的存在主要会使细胞表面发生褶皱,变得粗糙,甚至使细胞破裂。能谱结果证明Cd2+在细胞表面的积累。细胞表面的—OH、—CH2—、CH2/CH3、—C—OH和PO等基团参与了菌体的降Cd2+过程。因此,肉葡萄球菌846具有一定的降镉潜力,为降镉乳酸菌的开发提供了依据。
This paper aimed to investigate the cadmium-lowering properties and mechanisms of Staphylococcus carnosus 846 with high cadmium tolerance. The effects of pH, Cd2+ concentration, bacterial concentration and other metal ions on its cadmium-lowering ability were analyzed, and the mechanism of cadmium-decrease was investigated by scanning electron microscopy, energy dispersive spectrometer (EDS) and mid-infrared Fourier spectroscopy. The results showed that the cadmium-lowering efficiency of the strain reached the maximum at pH 5, with the Cd2+ concentration for 50 mg/L and the concentration of the bacterium for 1 g/L. The presence of other metal ions such as Mg2+, Pb2+ and Zn2+ reduced the Cd2+ lowering effect of the strain, while the presence of Cr6+ enhanced its Cd2+ lowering ability. The presence of Cd2+ mainly caused the cell surface wrinkling, rough and even cell rupture. The EDS results demonstrated the Cd2+ accumulated mainly on the cell surface. The —OH, —CH2—, CH2/CH3, —C—OH and PO groups on the cell surface were involved in the Cd2+ lowering process of S. carnosus 846. Therefore, S. carnosus 846 were demonstrated having cadmium reduction potential, which provided a basis for the development of cadmium-lowering lactic acid bacteria.
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