脱落酸(S-abscisic acid,S-ABA)是五大植物生长调节剂之一,现代农业广泛使用于农业生产,具有增强植物抗逆胁迫,保花保果,促进果实转色、增糖降酸等诸多功能。多种植物病原真菌可以合成,现广泛应用于生产的为灰葡萄孢霉菌(Botrytis cinerea),该文以使用响应面分析方法,考察碳氮比、初始pH、CaCO3质量浓度、搅拌转速、通气条件、接种量等关键营养条件和工艺条件对Botrytis cinerea菌体形态和产S-ABA的影响。确定最佳发酵条件为:碳氮比25、搅拌强度535 r/min、初始pH 5.1、接种量11%、通气量1.0 vvm,最佳菌丝球尺寸0.95 mm。此条件下S-ABA产量提升了约8.23%。
Abscisic acid (S-ABA) is one of the five major plant growth regulators, widely used in modern agriculture. It has many functions such as enhancing plant resistance to stress, preserving flowers and fruits, promoting fruit color change, increasing sugar, and reducing acid. Multiple plant pathogenic fungi can be synthesized, and Botrytis cinerea is widely used for production. This article uses response surface methodology to investigate the effects of key nutritional and process conditions such as carbon nitrogen ratio, initial pH, CaCO3 content, stirring speed, ventilation conditions, and inoculation amount on the morphology and S-ABA production of B. cinerea. The optimal fermentation conditions were determined as follows: Carbon nitrogen ratio of 25, stirring intensity of 535 r/min, initial pH of 5.1, inoculation amount of 11%, ventilation rate of 1.0 vvm, and optimal mycelium ball size of 0.95 mm. Under this condition, the production of S-ABA increased by approximately 8.23%.
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