该研究以蛋清蛋白作为品质改良剂制备低脂凝固型酸奶,探究乳蛋白与蛋清蛋白比例、总蛋白质含量和蔗糖含量对凝固型酸奶pH、硬度、流变学特性、水分状态及感官品质的影响。蛋清蛋白添加比例的升高使得酸奶pH、硬度、储能模量、表观黏度和持水性增加。总蛋白质量分数2.5%时无法形成凝固酸奶,总蛋白质量分数为3.5%和4.5%的酸奶能良好凝固。添加蔗糖能够改善酸奶的感官品质,结果显示添加蔗糖质量分数为6%的酸奶具有最高的感官评分。相关性分析表明,酸奶质地与其储能模量、pH呈正相关,与持水能力呈负相关。这些结果表明,蛋清蛋白能够改善低脂凝固型酸奶的品质。
In this study, low-fat set yogurt was prepared with egg white protein as a quality improver. The influences of the ratio of whey protein to egg white protein, total protein content and sucrose content on the pH, firmness, rheological properties, water state and sensory quality of set yogurt was investigated. The increase of egg white protein ratio increased the pH, firmness, storage modulus and apparent viscosity and water-holding capacity of yogurt. The yogurt with a total protein content of 2.5% failed to set, while the yogurt can set well when the total protein content of yogurt achieved 3.5% and 4.5%. The addition of sucrose improved the sensory quality of yogurt, it turned out that yogurt with a sucrose content of 6% had the highest sensory score. Correlation analysis showed that the texture of yogurt was positively correlated with storage modulus, firmness and pH, and negatively correlated with water-holding capacity. These results suggest that the egg white protein is effective to improve the quality of low-fat set yogurt.
[1] MACORI G, COTTER P D.Novel insights into the microbiology of fermented dairy foods[J].Current Opinion in Biotechnology, 2018, 49:172-178.
[2] TIAN Z C, ZHANG Y Y, ZHANG H M, et al.Transcriptional regulation of milk fat synthesis in dairy cattle[J].Journal of Functional Foods, 2022, 96:105208.
[3] ZOBEL E H, HANSEN T W, ROSSING P, et al.Global changes in food supply and the obesity epidemic[J].Current Obesity Reports, 2016, 5(4):449-455.
[4] LI H J, ZHANG L L, JIA Y Y, et al.Application of whey protein emulsion gel microparticles as fat replacers in low-fat yogurt:Applicability of vegetable oil as the oil phase[J].Journal of Dairy Science, 2022, 105(12):9404-9416.
[5] 李星, 王浩, 张丽华, 等.4种禽蛋中氨基酸成分的测定及分析[J].食品研究与开发, 2022, 43(4):158-168.
LI X, WANG H, ZHANG L H, et al.Measurement and analysis of amino acids in four types of poultry eggs[J].Food Research and Development, 2022, 43(4):158-168.
[6] ARENA S, RENZONE G, SCALONI A.A multi-approach peptidomic analysis of hen egg white reveals novel putative bioactive molecules[J].Journal of Proteomics, 2020, 215:103646.
[7] LI J H, LI X, WANG C Y, et al.Characteristics of gelling and water holding properties of hen egg white/yolk gel with NaCl addition[J].Food Hydrocolloids, 2018, 77:887-893.
[8] WEIJERS M, VAN DE VELDE F, STIJNMAN A, et al.Structure and rheological properties of acid-induced egg white protein gels[J].Food Hydrocolloids, 2006, 20(2-3):146-159.
[9] PU X L, ZHANG M Y, LIN X D, et al.Development and characterization of acid-induced whey protein concentrate and egg white protein composite gel[J].LWT, 2022, 164:113624.
[10] MINE Y.Recent advances in egg protein functionality in the food system[J].World's Poultry Science Journal, 2002, 58(1):31-39.
[11] TABILO-MUNIZAGA G, BARBOSA-CÁNOVAS G V.Pressurized and heat-treated surimi gels as affected by potato starch and egg white:Microstructure and water-holding capacity[J].LWT, 2005, 38(1):47-57.
[12] WANG J, SU Y J, GU L P, et al.The inhibition of cell-free supernatants of several lactic acid bacteria on the selected psychrophilic spoilage bacteria in liquid whole egg[J].Food Control, 2021, 123(5):107753.
[13] WU S, LI D, LI S J, et al.Effects of incubation temperature, starter culture level and total solids content on the rheological properties of yogurt[J].International Journal of Food Engineering, 2009, 5(2).
[14] JIANG Y W, JIA J, XIONG D D, et al.Effects of short-term fermentation with lactic acid bacteria on egg white:Characterization, rheological and foaming activities[J].Food Hydrocolloids, 2020, 101(4):105507.
[15] CHEN J, WANG J, XU L L, et al.Study on gel properties of lysozyme-free egg white before and after Lactiplantibacillus plantarum fermentation[J].Journal of the Science of Food and Agriculture, 2022, 102(13):5618-5627.
[16] 惠丹阳, 华舒雨, 唐裕芳, 等.大豆蛋白部分替代牛乳蛋白对酸奶理化性质及结构的影响[J].食品与发酵工业, 2023, 49(15):122-128.
HUI D Y, HUA S Y, TANG Y F, et al.Effect of partial replacement of milk protein by soy protein on the physicochemical properties and structure of yogurt[J].Food and Fermentation Industries, 2023, 49(15):122-128.
[17] 赵晓, 杜琨, 张媛.菊粉对合生元酸奶质构、流变学特性和微观结构的影响[J].食品工业科技, 2023, 44(1):72-77.
ZHAO X, DU K, ZHANG Y.Effect of inulin on the texture, rheological properties, and microstructure of synbiotic yogurt[J].Science and Technology of Food Industry, 2023, 44(1):72-77.
[18] DICKINSON E, MATIO MERINO L.Effect of sugars on the rheological properties of acid caseinate-stabilized emulsion gels[J].Food Hydrocolloids, 2002, 16(4):321-331.
[19] ZHAO J, BHANDARI B, GAIANI C, et al.Physicochemical and microstructural properties of fermentation-induced almond emulsion-filled gels with varying concentrations of protein, fat and sugar contents[J].Current Research in Food Science, 2021, 4(3):577-587.
[20] DEVNANI B, ONG L, KENTISH S E, et al.Physicochemical and rheological properties of commercial almond-based yoghurt alternatives to dairy and soy yoghurts[J].Future Foods, 2022, 6:100185.
[21] SALOMONSEN T, SEJERSEN M T, VIERECK N, et al.Water mobility in acidified milk drinks studied by low-field 1H NMR[J].International Dairy Journal, 2007, 17(4):294-301.
[22] GE Z W, BAO X, LI Z Y, et al.In situ exopolysaccharides produced by Lactobacillus helveticus MB2-1 and its effect on gel properties of Sayram ketteki yoghurt[J].International Journal of Biological Macromolecules, 2022, 208:314-323.
[23] 程欣玥, 孙小茜, 石静静, 等.增稠剂促进酸奶凝胶的机理及应用研究进展[J].食品与发酵工业, 2022, 48(21):325-332.
CHENG X Y, SUN X Q, SHI J J, et al.Research progress on the mechanism and application of yogurt gelation promoted by thickener[J].Food and Fermentation Industries, 2022, 48(21):325-332.