食品与发酵工业 >

2024 , Vol. 50 >Issue 4: 126 - 132

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034301

研究报告

低温等离子体对金鲳鱼肌原纤维蛋白结构特性的影响

  • 徐杰 ,
  • 徐雯晴 ,
  • 孙钦秀 ,
  • 王泽富 ,
  • 高加龙 ,
  • 吉宏武 ,
  • 刘书成
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东 湛江,524088)
    2(江苏省海洋生物资源与环境重点实验室,江苏海洋大学,江苏 连云港,222005)
    3(海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁 大连,116034)
第一作者:硕士研究生(孙钦秀讲师为通信作者,E-mail:sunqinxiugo@163.com)

收稿日期: 2022-11-14

  修回日期: 2022-12-12

  网络出版日期: 2024-03-15

基金资助

江苏省海洋生物资源与环境重点实验室开放课题(SH20211202);广东省普通高校青年创新人才类项目(2020KQNCX028);广东普通高等学校海洋食品绿色加工技术研究团队(2019KCXTD011)

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Effect of cold plasma on structural properties of golden pompano (Trachinotus ovatus) myofibrillar proteins

  • XU Jie ,
  • XU Wenqing ,
  • SUN Qinxiu ,
  • WANG Zefu ,
  • GAO Jialong ,
  • JI Hongwu ,
  • LIU Shucheng
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China)
    3(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2022-11-14

  Revised date: 2022-12-12

  Online published: 2024-03-15

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摘要

采用不同电压(10、20、30 kV)处理金鲳鱼,分析处理后鱼肉肌原纤维蛋白的Ca2+-ATP酶活性、肌原纤维小片化指数(myofibrillary fragmentation index,MFI)、三氯乙酸(trichloroacetic acid,TCA)溶解肽、总氨基酸含量、表面疏水性、紫外吸收光谱、红外光谱和SDS-PAGE,探究低温等离子体(cold plasma,CP)处理对金鲳鱼肌原纤维蛋白结构特性的影响。结果表明,随着CP处理电压的增加,金鲳鱼肉的MFI、TCA溶解肽和总氨基酸含量显著升高(P<0.05),Ca2+-ATP酶活力显著下降(P<0.05),表明肌原纤维蛋白变性程度随电压增加而加深。同时,表面疏水性和紫外吸收强度增加,但是红外光谱和SDS-PAGE未发生显著变化,表明过高电压CP处理会促进蛋白质变性,三级结构发生一定程度舒展,但是对一、二级结构影响较小。综上,在利用CP对水产品进行杀菌时应该避免过高电压导致的水产蛋白质的变性。

关键词： 低温等离子体; 金鲳鱼; 肌原纤维蛋白; 蛋白结构

本文引用格式

徐杰 , 徐雯晴 , 孙钦秀 , 王泽富 , 高加龙 , 吉宏武 , 刘书成 . 低温等离子体对金鲳鱼肌原纤维蛋白结构特性的影响[J]. 食品与发酵工业, 2024 , 50(4) : 126 -132 . DOI: 10.13995/j.cnki.11-1802/ts.034301

Abstract

To investigate the effects of cold plasma (CP) on the structural properties of golden pompano (Trachinotus ovatus) myofibrillar protein (MP), the fish was treated with cold plasma with different voltage (10, 20, and 30 kV), the Ca2+-ATPase activity, myofibrillary fragmentation index (MFI), trichloroacetic acid (TCA) soluble peptide, total amino acid content, surface hydrophobicity, ultraviolet absorption spectrum, infrared spectrum, and SDS-PAGE of myofibrillar protein were measured after treatment. Results showed that the contents of MFI, TCA soluble peptide, and total amino acid content significantly increased (P<0.05), while Ca2+-ATPase activity significantly decreased with the increasing voltage of CP treatment, indicating that the degree of MP denaturation increased with increasing voltage. Meanwhile, the surface hydrophobicity and ultraviolet absorption intensity increased, however, there was no significant change in protein secondary structure and SDS-PAGE, indicating that high-voltage CP treatment could promote protein denaturation and a certain degree of tertiary structure stretching, but had little effect on the primary and secondary structures. Thus, it is necessary to select a suitable voltage CP to sterilize the fish, which could avoid the denaturation of aquatic proteins caused by excessive voltage.

Key words： cold plasma; Trachinotus ovatus; myofibrillar proteins; protein structure

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