综述与专题评论

基于铁蛋白纳米笼构建传感元件及其在食品检测中的研究进展

  • 韩雪儿 ,
  • 谢江 ,
  • 虎梦吉 ,
  • 马良 ,
  • 郭婷 ,
  • 张宇昊 ,
  • 尚永彪 ,
  • 陈海
展开
  • 1(西南大学 食品科学学院,重庆,400715)
    2(川渝共建特色食品重庆市重点实验室,重庆,400715)
    3(发光分析和分子传感教育部重点实验室,重庆,400715)
第一作者:硕士研究生(陈海副教授和尚永彪教授为共同通信作者,E-mail:chenhai2509@swu.edu.cn;252143819@qq.com)

收稿日期: 2022-12-01

  修回日期: 2023-01-14

  网络出版日期: 2024-03-15

基金资助

国家自然科学基金青年项目(32201928);重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX055);十四五国家重点研发计划项目(2021YFD21001005)

Research progress of ferritin nanocage-based sensors and their application for detection of food-hazardous substances

  • HAN Xueer ,
  • XIE Jiang ,
  • HU Mengji ,
  • MA Liang ,
  • GUO Ting ,
  • ZHANG Yuhao ,
  • SHANG Yongbiao ,
  • CHEN Hai
Expand
  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)
    3(Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing 400715, China)

Received date: 2022-12-01

  Revised date: 2023-01-14

  Online published: 2024-03-15

摘要

铁蛋白(ferritin)是由24个亚基自组装而成的中空笼形结构蛋白,具有良好的水溶性、稳定性、生物相容性和可逆自组装特性。在生物体内,铁蛋白在铁的吸收、转运、氧化和贮存中扮演重要角色,具有调节体内铁代谢平衡的功能。近年来,由于铁蛋白具有纳米尺度的笼形结构,而且容易通过化学和生物等方法进行结构改造和修饰,使得其被广泛用于检测传感元件的构建。该文对铁蛋白分子的结构与功能进行简要介绍,总结了铁蛋白在构建检测传感元件中的2种基本策略,并重点回顾了基于铁蛋白传感元件在食品真菌毒素、重金属离子、病毒、过氧化氢等有毒有害物质检测中的研究进展,以期为铁蛋白在食品检测领域的应用提供研究思路。

本文引用格式

韩雪儿 , 谢江 , 虎梦吉 , 马良 , 郭婷 , 张宇昊 , 尚永彪 , 陈海 . 基于铁蛋白纳米笼构建传感元件及其在食品检测中的研究进展[J]. 食品与发酵工业, 2024 , 50(3) : 328 -335 . DOI: 10.13995/j.cnki.11-1802/ts.034501

Abstract

Ferritin is composed of 24 subunits that are self-assembled into a cage-like protein nanocage and possess good water solubility, stability, biocompatibility, and reversible self-assembly features. In biology, ferritin proteins play an important role in iron absorption, transport, oxidation, and storage, and participate in iron metabolism. In recent years, due to their nanoscale particle size and robust structure that is amenable to chemical and genetic engineering, ferritin nanocages have been widely used for the construction of biosensors in food, medical, and material sciences. In this paper, by starting with a brief introduction to the structure and function of ferritin nanocages, two basic strategies for the construction of ferritin-based sensors were summarized. Subsequently, the research progress on the ferritin nanocage-based sensors for the detection of food mycotoxins and harmful substances including food toxins, heavy metal ions, viruses, and hydrogen peroxide were reviewed. The purpose of this article was to provide insight into the construction of a ferritin-based sensor for the detection of food hazardous substances.

参考文献

[1] 鄢雷娜, 吴鑫, 段和祥, 等.高效液相色谱法同时测定抗疲劳功能食品中4种功效成分的研究[J].食品安全质量检测学报, 2020, 11(1):170-174.
YAN L N, WU X, DUAN H X, et al.Determination of 4 functional compositions in antifatigue health foods by high performance liquid chromatography[J].Journal of Food Safety & Quality, 2020, 11(1):170-174.
[2] 石勤艳, 姚颖辉, 侯义德, 等.气相色谱法测定保康茶叶中有机氯农药残留[J].食品安全质量检测学报, 2020, 11(1):158-164.
SHI Q Y, YAO Y H, HOU Y D, et al.Determination of organochlorine pesticide residues in Baokang tea samples by gas chromatography[J].Journal of Food Safety & Quality, 2020, 11(1):158-164.
[3] 李泽冉, 荣维广, 陈蓓, 等.气相色谱-质谱法同时测定鲜猪肉中氟乙酰胺和毒鼠强[J].江苏预防医学, 2021, 32(6):679-681.
LI Z R, RONG W G, CHEN B, et al.Simultaneous determination of fluoracetamide and tetramine infresh pork by gas chromatography-mass spectrometry[J].Jiangsu Journal of Preventive Medicine, 2021, 32(6):679-681.
[4] TANG Y, HUANG X H, WANG X L, et al.G-quadruplex DNAzyme as peroxidase mimetic in a colorimetric biosensor for ultrasensitive and selective detection of trace tetracyclines in foods[J].Food Chemistry, 2022, 366:130560.
[5] LAUFBRERGER V.Sur la cristallisation de la ferritin[J].Bulletin de la Societe de Chimie, 1937, 19:1575-1582.
[6] NGUYEN T H A, NGUYEN V C, PHAN T N H, et al.Novel biogenic silver and gold nanoparticles for multifunctional applications:Green synthesis, catalytic and antibacterial activity, and colorimetric detection of Fe(III) ions[J].Chemosphere, 2022, 287:132271.
[7] MADANODAYA S, AGNIHOTRA SRIKANTH R, BRENT A, et al.An electrochemical molecularly imprinted polymer sensor for rapid and selective food allergen detection[J].Food Chemistry, 2020, 344:128648.
[8] HARRISON P M, AROSIO P.The ferritins:Molecular properties, iron storage function and cellular regulation[J].Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1996, 1275(3):161-203.
[9] AROSIO P, INGRASSIA R, CAVADINI P.Ferritins:A family of molecules for iron storage, antioxidation and more[J].Biochimica et Biophysica Acta (BBA) - General Subjects, 2009, 1790(7):589-599.
[10] BRIAT J F, CELLIER F, GAYMARD F.Ferritins and Iron Accumulation in Plant Tissues[M].Iron Nutrition in Plants and Rhizospheric Microorganisms.Dordrecht:Springer Netherlands, 2006:341-357.
[11] AKANDA M R, JU H X.Ferritin-triggered redox cycling for highly sensitive electrochemical immunosensing of protein[J].Analytical Chemistry, 2018, 90(13):8028-8034.
[12] ZHEN Z P, TANG W, GUO C L, et al.Ferritin nanocages to encapsulate and deliver photosensitizers for efficient photodynamic therapy against cancer[J].ACS Nano, 2013, 7(8):6988-6996.
[13] LIN X, XIE J, NIU G, et al.Chimeric ferritin nanocages for multiple function loading and multimodal imaging[J].Nano Letters, 2011, 11(2):814-819.
[14] MING T H, HUAN H S, SU C, et al.Structural comparison of two ferritins from the marine invertebrate Phascolosoma esculenta[J].FEBS Open Bio, 2021, 11(3):793-803.
[15] YANG R, TIAN J, LIU Y Q, et al.Interaction mechanism of ferritin protein with chlorogenic acid and iron ion:The structure, iron redox, and polymerization evaluation[J].Food Chemistry, 2021, 349:129144.
[16] ZHAO G H.Phytoferritin and its implications for human health and nutrition[J].Biochimica et Biophysica Acta (BBA) - General Subjects, 2010, 1800(8):815-823.
[17] MAITY B, LI Z P, NIWASE K, et al.Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution[J].Physical Chemistry Chemical Physics:PCCP, 2020, 22(33):18562-18572.
[18] UCHIDA M, KANG S, REICHHARDT C, et al.The ferritin superfamily:Supramolecular templates for materials synthesis[J].Biochimica et Biophysica Acta (BBA) - General Subjects, 2010, 1800(8):834-845.
[19] ZHANG Z J, ZAWOROTKO M J.Template-directed synthesis of metal-organic materials[J].Chemical Society Reviews, 2014, 43(16):5444-5455.
[20] SONG N N, ZHANG J L, ZHAI J, et al.Ferritin:A multifunctional nanoplatform for biological detection, imaging diagnosis, and drug delivery[J].Accounts of Chemical Research, 2021, 54(17):3313-3325.
[21] SÁNCHEZ P, VALERO E, GÁLVEZ N, et al.MRI relaxation properties of water-soluble apoferritin-encapsulated gadolinium oxide-hydroxide nanoparticles[J].Dalton Transactions, 2009(5):800-804.
[22] JIANG B, YAN L A, ZHANG J L, et al.Biomineralization synthesis of the cobalt nanozyme in SP94-ferritin nanocages for prognostic diagnosis of hepatocellular carcinoma[J].ACS Applied Materials & Interfaces, 2019, 11(10):9747-9755.
[23] NAITO M, IWAHORI K, MIURA A, et al.Circularly polarized luminescent CdS quantum dots prepared in a protein nanocage[J].Angewandte Chemie, 2010, 49(39):7006-7009.
[24] XING R M, WANG X Y, YAN L L, et al.Fabrication of water soluble and biocompatible CdSe nanoparticles in apoferritin with the aid of EDTA[J].Dalton Transactions, 2009(10):1710-1713.
[25] LI M, VIRAVAIDYA C, MANN S.Polymer-mediated synthesis of ferritin-encapsulated inorganic nanoparticles[J].Small, 2007, 3(9):1477-1481.
[26] LIU G D, WANG J, WU H, et al.Versatile apoferritin nanoparticle labels for assay of protein[J].Analytical Chemistry, 2006, 78(21):7417-7423.
[27] LIU G D, WU H, DOHNALKOVA A, et al.Apoferritin-templated synthesis of encoded metallic phosphate nanoparticle tags[J].Analytical Chemistry, 2007, 79(15):5614-5619.
[28] ZHANG J L, CHENG D F, HE J Y, et al.Cargo loading within ferritin nanocages in preparation for tumor-targeted delivery[J].Nature Protocols, 2021, 16(10):4878-4896.
[29] KLEM M T, MOSOLF J, YOUNG M, et al.Photochemical mineralization of europium, titanium, and iron oxyhydroxide nanoparticles in the ferritin protein cage[J].Inorganic Chemistry, 2008, 47(7):2237-2239.
[30] CHEN H, MA L, ZHANG Y. Ferritin-catalyzed synthesis of ferrihydrite nanoparticles with high mimetic peroxidase activity for biomolecule detection[J]. RSC advances, 2021, 11(42): 26211-26217.
[31] TANG Z, WU H, ZHANG Y, et al. Enzyme-mimic activity of ferric nano-core residing in ferritin and its biosensing applications[J]. Analytical Chemistry, 2011, 83(22): 8611-8616.
[32] JIANG X, SUN C J, GUO Y, et al.Peroxidase-like activity of apoferritin paired gold clusters for glucose detection[J].Biosensors and Bioelectronics, 2015, 64:165-170.
[33] KISHIDA Y, OLSEN B R, BERG R A, et al.Two improved methods for preparing ferritin-protein conjugates for electron microscopy[J].The Journal of Cell Biology, 1975, 64(2):331-339.
[34] KRAMER R M, LI C, CARTER D C, et al.Engineered protein cages for nanomaterial synthesis[J].Journal of the American Chemical Society, 2004, 126(41):13282-13286.
[35] LIU M M, ZHU Y, JIN D, et al.Hemin-caged ferritin acting as a peroxidase-like nanozyme for the selective detection of tumor cells[J].Inorganic Chemistry, 2021, 60(19):14515-14519.
[36] 刘博, 张晨曦, 臧佳辰, 等.铁蛋白纳米载体在营养与健康领域的应用研究进展[J].食品科学, 2022, 43(15):302-311.
LIU B, ZHANG C X, ZANG J C, et al.Review of ferritin nanocarrier applications in the field of nutrition and health[J].Food Science, 2022, 43(15):302-311.
[37] JUTZ G, BÖKER A.Bionanoparticles as functional macromolecular building blocks: A new class of nanomaterials[J].Polymer, 2011, 52(2):211-232.
[38] TERASHIMA M, UCHIDA M, KOSUGE H, et al.Human ferritin cages for imaging vascular macrophages[J].Biomaterials, 2011, 32(5):1430-1437.
[39] ZHAO J, LIU M L, ZHANG Y Y, et al.Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection[J].Analytica Chimica Acta, 2013, 759:53-60.
[40] FERNÁNDEZ B, GÁLVEZ N, SÁNCHEZ P, et al.Fluorescence resonance energy transfer in ferritin labeled with multiple fluorescent dyes[J].JBIC Journal of Biological Inorganic Chemistry, 2008, 13(3):349-355.
[41] KANG H J, KANG Y J, LEE Y M, et al.Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform[J].Biomaterials, 2012, 33(21):5423-5430.
[42] KIM S E, AHN K Y, PARK J S, et al.Fluorescent ferritin nanoparticles and application to the aptamer sensor[J].Analytical Chemistry, 2011, 83(15):5834-5843.
[43] 杨婷婷, 严艺琳, 石文婷, 等.粮食中重金属污染的快速检测研究进展[J].粮食与食品工业, 2019, 26(2):56-60.
YANG T T, YAN Y L, SHI W T, et al.Research progress on rapid detection of heavy metals in grain[J].Cereal & Food Industry, 2019, 26(2):56-60.
[44] MIZANUR RAHMAN G M, WOLLE M M, FAHRENHOLZ T, et al.Measurement of mercury species in whole blood using speciated isotope dilution methodology integrated with microwave-enhanced solubilization and spike equilibration, headspace-solid-phase microextraction, and GC-ICP-MS analysis[J].Analytical Chemistry, 2014, 86(12):6130-6137.
[45] RUIZ-DE-CENZANO M, ROCHINA-MARCO A, CERVERA M L, et al.Speciation of methylmercury in market seafood by thermal degradation, amalgamation and atomic absorption spectroscopy[J].Ecotoxicology and Environmental Safety, 2014, 107:90-96.
[46] ARANDA P R, GIL R A, MOYANO S, et al.Slurry sampling in serum blood for mercury determination by CV-AFS[J].Journal of Hazardous Materials, 2009, 161(2-3):1399-1403.
[47] 张涛, 苏倡, 刘艳, 等.泥蚶(Tegillarca granosa)重组铁蛋白富集重金属离子的特性及化学传感器的研究[J].海洋与湖沼, 2017, 48(4):870-876.
ZHANG T, SU C, LIU Y, et al.Research of characteristics of enrichment of heavy metals by recombinant ferritin from Tegillarca granosa[J].Oceanologia et Limnologia Sinica, 2017, 48(4):870-876.
[48] 沈洋, 胡继文, 刘婷婷, 等.纳米光学传感器用于检测汞离子[J].化学进展, 2019, 31(4):536-549.
SHEN Y, HU J W, LIU T T, et al.Colorimetric and fluorogenic chemosensors for mercury ion based on nanomaterials[J].Progress in Chemistry, 2019, 31(4):536-549.
[49] WANG Y J, CHEN H, ZANG J C, et al.Re-designing ferritin nanocages for mercuric ion detection[J].The Analyst, 2019, 144(19):5890-5897.
[50] 白艺珍, 李培武, 丁小霞, 等.我国粮油作物产品真菌毒素风险评估现状与对策探讨[J].农产品质量与安全, 2015(5):54-58.
BAI Y Z, LI P W, DING X X, et al.Present situation and countermeasures of mycotoxin risk assessment of grain and oil crops in China[J].Quality and Safety of Agro-Products, 2015(5):54-58.
[51] 周晓文. 细交链孢菌酮酸单克隆抗体的制备及其icELISA方法的建立[D].广州:华南农业大学, 2017.
ZHOU X W.Development of monoclonal antibody against tenuazonic acid[D].Guangzhou:South China Agricultural University, 2017.
[52] 吴希, 邢家溧, 郑睿行, 等.超高效液相色谱-串联质谱法快速检测麦类中典型链格孢霉毒素[J].食品科学, 2022, 43(12):317-324.
WU X, XING J L, ZHENG R X, et al.Rapid determination of typical Alternaria toxins in wheat by ultra-high performance liquid chromatography tandem mass spectrometry[J].Food Science, 2022, 43(12):317-324.
[53] WANG F, LI Z F, WAN D B, et al.Enhanced non-toxic immunodetection of Alternaria mycotoxin tenuazonic acid based on ferritin-displayed anti-idiotypic nanobody-nanoluciferase multimers[J].Journal of Agricultural and Food Chemistry, 2021, 69(16):4911-4917.
[54] CHAVAN S G, YAGATI A K, MOHAMMADNIAEI M, et al.Robust bioengineered apoferritin nanoprobes for ultrasensitive detection of infectious pancreatic necrosis virus[J].Analytical Chemistry, 2019, 91(9):5841-5849.
[55] 赵国有, 林滢, 于维森.高致病性禽流感病毒H5N1核酸检测的风险评估[J].中国卫生检验杂志, 2021, 31(19):2431-2433.
ZHAO G Y, LIN Y, YU W S.Risk assessment of H5N1 nucleic acid detection of highly pathogenic avian influenza virus[J].Chinese Journal of Health Laboratory Technology, 2021, 31(19):2431-2433.
[56] FAN K L, JIANG B, GUAN Z, et al.Fenobody:A ferritin-displayed nanobody with high apparent affinity and half-life extension[J].Analytical Chemistry, 2018, 90(9):5671-5677.
[57] RAFIPOUR R, KASHANIAN S, ABASI TARIGHAT F.Sensitive electrochemical biosensing of H2O2 based on cobalt nanoparticles synthesised in iron storage protein molecules, ferritin[J].IET Nanobiotechnology, 2014, 8(4):196-200.
[58] TAMLEH Z, RAFIPOUR R, KASHANIAN S.Protein-based nanobiosensor for electrochemical determination of hydrogen peroxide[J].Russian Journal of Electrochemistry, 2019, 55(10):962-969.
[59] WANG L, WANG J K, NI P J, et al.Ferritin-mediated biomimetic synthesis of bimetallic Au-Ag nanoparticles on graphene nanosheets for electrochemical detection of hydrogen peroxide[J].Functional Materials Letters, 2015, 8(4):1550044.
文章导航

/