食品与发酵工业 >

2024 , Vol. 50 >Issue 5: 274 - 279

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035552

研究报告

膜醭毕赤酵母蛋白复合膜对桃采后果腐病的抑制效果及机制

  • 陈海艺 ,
  • 徐悦 ,
  • 刘艺璇 ,
  • 张亚敏 ,
  • 郭红莲
展开
  • (天津科技大学 食品科学与工程学院,天津,300457)
第一作者：陈海艺(硕士研究生)和徐悦(硕士研究生)为共同第一作者(郭红莲副教授为通信作者,E-mail:guohonglian@tust.edu.cn)

收稿日期: 2023-03-21

  修回日期: 2023-04-19

  网络出版日期: 2024-04-09

基金资助

天津市食品质量与健康重点实验室基金项目(TJS202101);天津科技大学大学生创新训练项目(202210057089)

收起

Inhibitory effect and mechanism of Pichia membranaefaciens composite protein film on postharvest rot of peaches

  • CHEN Haiyi ,
  • XU Yue ,
  • LIU Yixuan ,
  • ZHANG Yamin ,
  • GUO Honglian
Expand
  • (College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)

Received date: 2023-03-21

  Revised date: 2023-04-19

  Online published: 2024-04-09

Fold

摘要

该文旨在探究膜醭毕赤酵母(Pichia membranaefaciens)蛋白复合膜对镰刀孢(Fusarium spp.)的体外抑菌机制及对桃果采后果腐病的防治效果。通过测定蛋白复合膜对镰刀孢孢子萌发及菌丝生长的影响,分析抑菌机理,并测定果实硬度、褐变度、可溶性固形物及维生素C含量,探究复合膜对桃果采后贮藏期品质的影响。结果显示,蛋白复合膜处理下,孢子萌发受抑,菌丝生长受阻,菌落生长抑制率达56.72%;菌体细胞膜透性增大导致胞内核酸、蛋白质外泄;扫描电镜下菌丝膨大、表面皱褶、内容物外泄;有伤接种实验表明蛋白复合膜能有效降低桃果发病率并延缓病斑扩展,维持桃果硬度和色泽,延缓可溶性固形物及维生素C含量降低。膜醭毕赤酵母蛋白复合膜作为一种天然生物抗菌膜,能有效控制桃果采后果腐病,维持桃果实生理品质。

关键词： 蛋白复合膜; 桃; 采后病害; 抑制机制; 生理品质

本文引用格式

陈海艺 , 徐悦 , 刘艺璇 , 张亚敏 , 郭红莲 . 膜醭毕赤酵母蛋白复合膜对桃采后果腐病的抑制效果及机制[J]. 食品与发酵工业, 2024 , 50(5) : 274 -279 . DOI: 10.13995/j.cnki.11-1802/ts.035552

Abstract

This study aims to explore the inhibition of composite film containing secretory protein of Pichia membranaefaciens against Fusarium spp. in vitro and its effect on the control of peach rot caused by Fusarium spp.Spore germination and mycelial growth of pathogen were measured and the biological quality of inoculated peach were tested to explore the preservation effect of film.Research has shown that the film inhibited the spore germination and mycelial growth of Fusarium spp.Effectively, the inhibition rate of colony growth reached to 56.72%.The increase of membrane permeability leaded to the leakage of nucleic acid and protein.SEM observed that the mycelium expanded and its surface became uneven.The inoculation test showed the composite film could effectively reduce the incidence of peach and delay the spread of lesion;maintain the hardness and color;delay the decrease of soluble solid and Vc content.Above all, the protein composite film can effectively control post-harvest decay and maintain quality of peach as a kind of natural biological antibacterial material.

Key words： protein composite film; peach; postharvest disease; antibacterial mechanism; physiological quality

参考文献

[1] 张娜, 郑香香, 于晋泽, 等.晚熟桃采后褐腐病致病菌的分离鉴定及拮抗菌对其防治效果的研究[J].食品工业科技, 2018, 39(2):102-107.
ZHANG N, ZHENG X X, YU J Z, et al.Isolation and identification of pathogen caused postharvest brown rot of late-ripening peach and bio-control effects of antagonistic bacteria[J].Science and Technology of Food Industry, 2018, 39(2):102-107.
[2] 肖烟云, 张婷婷, 林丽莎, 等.中国桃果实采后生理和病害研究进展[J].包装与食品机械, 2014, 32(3):45-51.
XIAO Y Y, ZHANG T T, LIN L S, et al.Advances in the studies on post-harvest physiology and post-harvest disease of Chinese peach fruit[J].Packaging and Food Machinery, 2014, 32(3):45-51.
[3] 霍若冰, 李洋, 徐曈晖, 等.鱼腥草提取液-壳聚糖抗菌复合膜对低温贮藏蓝莓的保鲜作用[J].现代食品科技, 2022, 38(8):153-162.
HUO R B, LI Y, XU T H, et al.The fresh-keeping effect of antibacterial Houttuynia cordata extract-chitosan composite film on blueberries stored at A low temperature[J].Modern Food Science and Technology, 2022, 38(8):153-162.
[4] 方静, 谷会, 姚全胜, 等.肉桂精油复合壳聚糖乳液对采后芒果炭疽病的防治效果及作用机制分析[J].热带作物学报, 2023,44(7):1460-1467.
FANG J, GU H, YAO Q S, et al.Control effect and mechanism of cinnamon essential oil compound chitosan lotion on postharvest mango anthracnose[J].Chinese Journal of Tropical Crops, 2023,44(7):1460-1467.
[5] WANG X H, SONG X J, ZHANG D J, et al.Preparation and characterization of natamycin-incorporated agar film and its application on preservation of strawberries[J].Food Packaging and Shelf Life, 2022, 32:100863.
[6] 王笑笑, 季小诗, 刘芳, 等.生防菌KD-16J的鉴定及其对番茄灰霉病的抑制作用[J].食品研究与开发, 2021, 42(3):182-188.
WANG X X, JI X S, LIU F, et al.Identification of biocontrol strain KD-16J and the effect on grey mould of tomatoes[J].Food Research and Development, 2021, 42(3):182-188.
[7] 季小诗, 郭红莲, 王笑笑, 等.拮抗菌发酵液对冬枣贮藏品质的影响[J].食品与发酵工业, 2021, 47(14):82-87.
JI X S, GUO H L, WANG X X, et al.Effects of an antagonistic yeast fermentation broth on the storage quality of winter jujube[J].Food and Fermentation Industries, 2021, 47(14):82-87.
[8] 杨昭辉, 王笑笑, 赵祺, 等.膜醭毕赤酵母菌外泌蛋白抑菌复合膜的制备及性能研究[J].食品与发酵工业, 2022, 48(12):215-219.
YANG Z H, WANG X X, ZHAO Q, et al.Preparation and properties of antibacterial composite membrane of secreted protein from Pichia membranaefaciens[J].Food and Fermentation Industries, 2022, 48(12):215-219.
[9] 戴希爽, 张忠, 毕阳, 等.沙枣胶/枯茗醛复合涂膜对番茄采后红粉病的抑制作用及机制[J].食品科学, 2022, 43(23):187-193.
DAI X S, ZHANG Z, BI Y, et al.Inhibitory effect and mechanism of composite coating containing oleaster gum and cuminal on postharvest pink mold rot of tomatoes[J].Food Science, 2022, 43(23):187-193.
[10] 代博仁, 申光辉, 刘海娜, 等.大蒜有机硫化物对解淀粉芽孢杆菌的抑菌作用及机理[J].华南农业大学学报, 2020, 41(4):95-103.
DAI B R, SHEN G H, LIU H N, et al.Antibacterial activity and mechanism of garlic organic sulfide against Bacillus amyloliquefaciens[J].Journal of South China Agricultural University, 2020, 41(4):95-103.
[11] PEREIRA F D, MENDES J M, LIMA I O, et al.Antifungal activity of geraniol and citronellol, two monoterpenes alcohols, against Trichophyton rubrum involves inhibition of ergosterol biosynthesis[J].Pharmaceutical Biology, 2015, 53(2):228-234.
[12] ZHAO L N, LAN C, TANG X Y, et al.Efficacy of Debaryomyce hansenii in the biocontrol for postharvest soft rot of strawberry and investigation of the physiological mechanisms involved[J].Biological Control, 2022, 174:105011.
[13] LIU Q, CUI X, SONG Z B, et al.Coating shiitake mushrooms (Lentinus edodes) with a polysaccharide from Oudemansiella radicata improves product quality and flavor during postharvest storage[J].Food Chemistry, 2021, 352:129357.
[14] 令利军, 程文婷, 蒋坤玲, 等.黄花蒿精油及其单体桉叶油醇对兰州百合采后病害的防治效果[J].西北农林科技大学学报(自然科学版), 2022, 50(8):77-87.
LING L J, CHENG W T, JIANG K L, et al.Control of postharvest diseases of Lanzhou lily using essential oil of Artemisia annua L.and its monomer eucalyptol[J].Journal of Northwest A & F University (Natural Science Edition), 2022, 50(8):77-87.
[15] VIRÁGH M, MARTON A, VIZLER C, et al.Insight into the antifungal mechanism of Neosartorya fischeri antifungal protein[J].Protein & Cell, 2015, 6(7):518-528.
[16] 李永春. 臭椿canthin-6-one的提取分离及对尖孢镰刀孢抑菌机制研究[D].哈尔滨:东北林业大学, 2021.
LI Y C.Extraction and isolation of canthin-6-one from Ailanthus altissima and its antifungal mechanism against Fusarium oxysporum[D].Harbin:Northeast Forestry University, 2021.
[17] JIAO W X, SHU C, LI X X, et al.Preparation of a chitosan-chlorogenic acid conjugate and its application as edible coating in postharvest preservation of peach fruit[J].Postharvest Biology and Technology, 2019, 154:129-136.
[18] 余慧, 吴唯可, 范楚宏, 等.丝素-壳聚糖复合涂膜对鲜切苹果保鲜性能的研究[J].中国食品添加剂, 2018(6):125-129.
YU H, WU W K, FAN C H, et al.Fresh-keeping effect of silk fibroin-chitosan film on fresh-cut apple[J].China Food Additives, 2018(6):125-129.
[19] YUAN Y H, WANG H, FU Y, et al.Sodium alginate/gum Arabic/glycerol multicomponent edible films loaded with natamycin:Study on physicochemical, antibacterial, and sweet potatoes preservation properties[J].International Journal of Biological Macromolecules, 2022, 213:1068-1077.
[20] 李结瑶, 罗文翰, 肖更生, 等.丁香/香茅精油复合涂膜对百香果采后保鲜的研究[J].食品与发酵工业, 2023, 49(4):59-67.
LI J Y, LUO W H, XIAO G S, et al.Effort of clove/citronella essential oil composite coating on preservation of passion fruit[J].Food and Fermentation Industries, 2023, 49(4):59-67.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司