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微生物合成乳酸的细胞工厂构建研究进展

  • 王晓娜 ,
  • 田开仁 ,
  • 吴昊 ,
  • 寇佳祥 ,
  • 乔建军 ,
  • 李艳妮
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  • 1(天津大学 化工学院,天津,300072)
    2(系统生物工程教育部重点实验室,天津,300072)
    3(天津大学 浙江研究院(绍兴),浙江 绍兴,312300)
第一作者:硕士研究生(李艳妮副研究员为通信作者,E-mail:liyanni@tju.edu.cn)

收稿日期: 2023-04-13

  修回日期: 2023-05-10

  网络出版日期: 2024-04-09

基金资助

国家重点研发计划项目(2019YFA0904104)

Research advances on the construction of cell factory for microbial synthesis of lactic acid

  • WANG Xiaona ,
  • TIAN Kairen ,
  • WU Hao ,
  • KOU Jiaxiang ,
  • QIAO Jianjun ,
  • LI Yanni
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  • 1(School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China)
    2(Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China)
    3(Zhejiang Institute of Tianjin University (Shaoxing), Shaoxing 312300, China)

Received date: 2023-04-13

  Revised date: 2023-05-10

  Online published: 2024-04-09

摘要

乳酸目前广泛应用于食品、医疗和化妆品行业,并因其作为可生物降解塑料聚乳酸的原料而备受关注。微生物发酵合成乳酸具有光学纯度高和底物成本低等优点,因此,研究人员利用合成生物学和代谢工程技术构建了新型微生物细胞工厂,以实现乳酸的高效合成。在此过程中,代谢通路的构建与优化、氧化还原平衡的调节、底物谱的拓宽以及细胞工厂鲁棒性的提高等方面是需要考虑的关键问题。文章详细介绍了微生物合成乳酸的细胞工厂构建研究进展,并对当前面临的挑战和未来的研究方向进行了探讨,旨在为工业微生物高效合成乳酸提供借鉴。

本文引用格式

王晓娜 , 田开仁 , 吴昊 , 寇佳祥 , 乔建军 , 李艳妮 . 微生物合成乳酸的细胞工厂构建研究进展[J]. 食品与发酵工业, 2024 , 50(5) : 317 -325 . DOI: 10.13995/j.cnki.11-1802/ts.035820

Abstract

Lactic acid is widely used in the food, medical, and cosmetic industries, and has gained attention as a raw material for biodegradable plastic polylactic acid.Microbial synthesis of lactic acid offers advantages such as high optical purity and low substrate cost.Therefore, researchers have utilized synthetic biology and metabolic engineering techniques to construct novel microbial cell factories capable of efficient lactic acid synthesis.During this process, the construction and optimization of metabolic pathways, regulation of redox balance, broadening of substrate spectrum, and improvement of cell factory robustness are key issues that need to be considered.In this review, a comprehensive overview of recent advances in the construction of lactic acid synthesis microbial cell factory is provided.Furthermore, the current challenges and future research directions are explored, aiming to provide guidance for the efficient synthesis of lactic acid by industrial microorganisms.

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