食品与发酵工业 >

2024 , Vol. 50 >Issue 6: 116 - 121

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035657

研究报告

低聚糖对植物乳杆菌AUH2103抑菌作用的影响

  • 严浩东 ,
  • 张臣臣 ,
  • 徐晶晶 ,
  • 陈大卫 ,
  • 康文丽 ,
  • 潘丽娜 ,
  • 唐溶雪 ,
  • 李威 ,
  • 顾瑞霞
展开
  • 1(扬州大学 食品科学与工程学院,江苏 扬州,225000)
    2(扬州大学,江苏省乳品生物技术与安全控制重点实验室,江苏 扬州,225000)
    3(澳优乳业(中国)有限公司,湖南 长沙,410127)
    4(人体微生态制品湖南省工程研究中心,湖南 长沙,410127)
第一作者:硕士研究生(顾瑞霞教授为通信作者,E-mail:rxgu@yzu.edu.cn)

收稿日期: 2023-03-29

  修回日期: 2023-04-23

  网络出版日期: 2024-04-17

基金资助

湖南省长沙市望城区科技计划重点研发计划项目(K221012);江苏省自然科学基金面上项目(BK20211325);扬州市-扬州大学市校合作共建科技创新平台(YZ2020265)

收起

Effect of oligosaccharides on antibacterial activity of Lactiplantibacillus plantarum AUH2103

  • YAN Haodong ,
  • ZHANG Chenchen ,
  • XU Jingjing ,
  • CHEN Dawei ,
  • KANG Wenli ,
  • PAN Lina ,
  • TANG Rongxue ,
  • LI Wei ,
  • GU Ruixia
Expand
  • 1(College of Food Science and Engineering, Yangzhou University, Yangzhou 225000, China)
    2(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou 225000, China)
    3(Ausnutria Dairy Corporation Ltd., Changsha 410127, China)
    4(Hunan Engineering Research Center of Human Microecological Products, Changsha 410127, China)

Received date: 2023-03-29

  Revised date: 2023-04-23

  Online published: 2024-04-17

Fold

摘要

对致病菌的抑制能力是评价益生菌功能特性的重要指标。该文使用单层平板扩散法和细胞黏附法测试了植物乳杆菌AUH2103的抑菌能力。结果显示,将碳源替换为低聚半乳糖(galactooligosaccharides,GOS)与低聚果糖(fructooligosaccharides,FOS)后,植物乳杆菌AUH2103的抑菌能力与在MRS中的结果无显著差异;而将碳源替换为2′-岩藻糖基乳糖(2′-focusllactose, 2′-FL)后,由于生长能力变弱导致抑菌效果不明显。在低聚糖存在的情况下,植物乳杆菌AUH2103对于Caco-2细胞的黏附能力变强;在植物乳杆菌AUH2103与低聚糖存在的情况下,致病菌与Caco-2细胞的黏附能力出现明显下降。该研究表明,在低聚糖存在的情况下,植物乳杆菌AUH2103可以通过抑制致病菌对于Caco-2细胞的黏附产生抑菌效果。

关键词： 低聚糖; 益生菌; 抑菌; 细胞黏附

本文引用格式

严浩东 , 张臣臣 , 徐晶晶 , 陈大卫 , 康文丽 , 潘丽娜 , 唐溶雪 , 李威 , 顾瑞霞 . 低聚糖对植物乳杆菌AUH2103抑菌作用的影响[J]. 食品与发酵工业, 2024 , 50(6) : 116 -121 . DOI: 10.13995/j.cnki.11-1802/ts.035657

Abstract

The ability to inhibit pathogenic bacteria is an important indicator to evaluate the functional properties of probiotics.In this paper, the inhibition ability of Lactiplantibacillus plantarum AUH2103 was tested using a monolayer plate diffusion method and a cell adhesion method.The results showed that after replacing the carbon source with oligogalactose (GOS) and oligofructose (FOS), the inhibition ability of L.plantarum AUH2103 was not significantly different from the results in MRS;In contrast, the replacement of the carbon source with 2′-fucosyl lactose (2′-FL) resulted in insignificant inhibition due to weakened growth capacity.The adhesion of L.plantarum AUH2103 to Caco-2 became stronger in the presence of oligosaccharides;In the presence of L.plantarum AUH2103 and oligosaccharides, the adhesion ability of pathogenic bacteria to Caco-2 appeared to be significantly reduced.This study shows that in the presence of oligosaccharides, L.plantarum AUH2103 can inhibit the adhesion of pathogenic bacteria to Caco-2 cells.

Key words： oligosaccharide; probiotics; antibacterial; cell adhesion

参考文献

[1] ECKBURG P B, BIK E M, BERNSTEIN C N, et al.Diversity of the human intestinal microbial flora[J].Science, 2005, 308(5728):1635-1638.
[2] FURCI F, CAMINATI M, CRISAFULLI E, et al.The intriguing possibility of using probiotics in allergen-specific immunotherapy[J].World Allergy Organization Journal, 2023, 16(2):100751.
[3] HANEISHI Y, FURUYA Y, HASEGAWA M, et al.Inflammatory bowel diseases and gut microbiota[J].International Journal of Molecular Sciences, 2023, 24(4):3817.
[4] QI Y, WANG X F.The role of gut microbiota in high-fat-diet-induced diabetes:Lessons from animal models and humans[J].Nutrients, 2023, 15(4):922.
[5] KAMADA N, CHEN G Y, INOHARA N, et al. Control of pathogens and pathobionts by the gut microbiota[J].Nature Immunology, 2013, 14:685-690.
[6] PIEWNGAM P, ZHENG Y, NGUYEN T H, et al.Pathogen elimination by probiotic Bacillus via signalling interference[J].Nature, 2018, 562:532-537.
[7] GIBSON G R, HUTKINS R, SANDERS M E, et al.Expert consensus document:The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics[J].Nature Reviews Gastroenterology and Hepatology, 2017, 14(8):491-502.
[8] ROBERFROID M, GIBSON G R, HOYLES L, et al.Prebiotic effects:Metabolic and health benefits[J].The British Journal of Nutrition, 2010, 104(Suppl 2):1-63.
[9] GARRIDO D, RUIZ-MOYANO S, LEMAY D G, et al.Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria[J].Scientific Reports, 2015, 5:13517.
[10] OOZEER R, VAN LIMPT K, LUDWIG T, et al. Intestinal microbiology in early life: Specific prebiotics can have similar functionalities as human-milk oligosaccharides1[J]. The American Journal of Clinical Nutrition, 2013, 98(2):561S-571S.
[11] RUIZ-PALACIOS G M, CERVANTES L E, RAMOS P, et al.Campylobacter jejuni binds intestinal H(O) antigen (Fuc alpha 1, 2Gal beta 1, 4GlcNAc), and fucosyloligosaccharides of human milk inhibit its binding and infection[J].The Journal of Biological Chemistry, 2003, 278(16):14112-14120.
[12] YU Z T, NANTHAKUMAR N N, NEWBURG D S.The human milk oligosaccharide 2′-fucosyllactose quenches Campylobacter jejuni-induced inflammation in human epithelial cells HEp-2 and HT-29 and in mouse intestinal Mucosa1[J].The Journal of Nutrition, 2016, 146(10):1980-1990.
[13] BOHORA A A, KOKATE S R, KHEDKAR S, et al.Antimicrobial activity of probiotics against endodontic pathogens:A preliminary study[J].Indian Journal of Medical Microbiology, 2019, 37(1):5-11.
[14] ALIZADEH BEHBAHANI B, NOSHAD M, FALAH F.Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15[J].Microbial Pathogenesis, 2019, 136:103677.
[15] SINGH T P, KAUR G, KAPILA S, et al.Antagonistic activity of Lactobacillus reuteri strains on the adhesion characteristics of selected pathogens[J].Frontiers in Microbiology, 2017, 8:486.
[16] 王鹏, 刚梦萱, 张臣臣, 等.母乳源乳酸菌的低聚糖利用特性研究[J].食品与发酵工业, 2022, 48(11):101-106.
WANG P, GANG M X, ZHANG C C, et al.Oligosaccharide utilization characteristics of lactic acid bacteria from breast milk[J].Food and Fermentation Industries, 2022, 48(11):101-106.
[17] COPPA G V, BRUNI S, ZAMPINI L, et al. Oligosaccharides of human milk inhibit the adhesion of listeria monocytogenes to Caco-2 cells[J].The Italian Journal of Pediatrics, 2003, 29(1):61-68.
[18] ELO S, SAXELIN M, SALMINEN S. Attachment of Lactobacillus casei strain GG to human colon carcinoma cell line Caco-2:Comparison with other dairy strains Lett[J].Letters in Applied Microbiology, 1991, 13(3):154-156.
[19] CHAUVIERE G, COCONNIER M H, KERNEIS S, et al.Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells[J].Journal of General Microbiology, 1992, 138(8):1689-1696.
[20] BERNET M F, BRASSART D, NEESER J R, et al.Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions[J].Applied and Environmental Microbiology, 1993,59(12):4121-4128.
[21] BEACHEY E H.Bacterial adherence:Adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces[J].The Journal of Infectious Diseases, 1981, 143(3):325-345.
[22] GRIGORYAN S, BAZUKYAN I, TRCHOUNIAN A.Aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo:A potential probiotic strain[J].Probiotics and Antimicrobial Proteins, 2018, 10(2):269-276.
[23] ENDO H, TAMURA K, FUKASAWA T, et al.Comparison of fructooligosaccharide utilization by Lactobacillus and Bacteroides species[J].Bioscience, Biotechnology, and Biochemistry, 2012, 76(1):176-179.
[24] FACINELLI B, MARINI E, MAGI G, et al.Breast milk oligosaccharides:Effects of 2′-fucosyllactose and 6′-sialyllactose on the adhesion of Escherichia coli and Salmonella fyris to Caco-2 cells[J].The Journal of Maternal-Fetal and Neonatal Medicine, 2019, 32(17):2950-2952.
[25] CILIEBORG M S, SANGILD P T, JENSEN M L, et al.α1,2-Fucosyllactose does not improve intestinal function or prevent Escherichia coli F18 diarrhea in newborn pigs[J].Journal of Pediatric Gastroenterology and Nutrition, 2017, 64(2):310-318.
[26] JANKOWSKA A, LAUBITZ D, ANTUSHEVICH H, et al.Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells[J].Journal of Biomedicine and Biotechnology, 2008, 2008:357964.
[27] JAYASHREE S, KARTHIKEYAN R, NITHYALAKSHMI S, et al.Anti-adhesion property of the potential probiotic strain Lactobacillus fermentum 8711 against methicillin-resistant Staphylococcus aureus (MRSA)[J].Frontiers in Microbiology, 2018, 9:411.
[28] COCONNIER M H, BERNET M F, KERNÉIS S, et al.Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion[J].FEMS Microbiology Letters, 1993, 110(3):299-305.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司