为探究NO对杏果实采后贮藏期间的抗氧化作用,实验以吊干杏为试材,采用外源NO处理,测定了杏果实采后硬度、呼吸强度、可溶性固形物(soluble solids,TSS)、可滴定酸(titratable acid,TA)和H2O2含量,抗坏血酸-谷胱甘肽(ascorbic acid-glutathione,AsA-GSH)循环中抗坏血酸(ascorbic acid,AsA)、还原型谷胱甘肽(reduced glutathione,GSH)的含量,脱氢抗坏血酸(dehydroascorbic acid,DHA)、氧化型谷胱甘肽(glutathione oxidized,GSSG)含量,抗坏血酸过氧化物酶(ascorbic acid peroxidase,APX)、谷胱甘肽还原酶(glutathione reductase,GR)、单脱氢抗坏血酸还原酶(monodehydroascorbate reductase,MDHAR)以及脱氢抗坏血酸还原酶(dehydroascorbate reductase,DHAR)的变化。结果表明,NO处理可延缓杏果实采后硬度下降,降低了呼吸强度和TA含量,提高TSS含量,较好地维持杏果实贮藏品质。同时,NO处理降低了杏果实、果皮和果肉中H2O2含量,增强了APX、GR、MDHAR和DHAR的活性,提高了AsA和GSH的含量,降低了DHA和GSSG含量。此外,NO处理后果皮抗氧化酶活性和H2O2含量均高于果肉。NO处理通过激活AsA-GSH循环关键抗氧化酶活性,提高了清除H2O2的效率,调节杏果实采后AsA-GSH循环,从而维持了杏果实采后贮藏品质,且果皮的响应快于果肉。
The test used to hang dried apricot as the test material and used nitric oxide (NO) treatment to determine the hardness, respiration strength, soluble solids (TSS), titratable acid (TA), and H2O2content, and change of ascorbic acid (AsA), reduced glutathione (GSH) content, dehydroascorbic acid (DHA), oxidized glutathione (GSSG), ascorbic acid peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) in the ascorbic acid-glutathione (AsA-GSH) cycle.Results showed that NO treatment could delay the decrease of postharvest hardness, reduce respiration intensity and TA, increase TSS content, and better maintain the storage quality of apricot fruit.At the same time, NO treatment reduced the content of H2O2 in the peel and pulp of apricot fruit, enhanced the activity of APX, GR, MDHAR, and DHAR, increased the content of AsA and GSH, and reduced the content of DHA and GSSG.In addition, the contents of antioxidant enzymes and H2O2 in the skin were higher than those in the pulp after NO treatment.NO treatment improved the efficiency of H2O2 removal and regulated the postharvest AsA-GSH cycle by activating AsA-GSH antioxidant enzyme activity, thereby maintaining the postharvest storage quality of apricot fruit, and the response of the peel was faster than that of the pulp.
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