食品与发酵工业 >

2024 , Vol. 50 >Issue 7: 92 - 98

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035769

研究报告

固态发酵制备黄精多糖的工艺优化、理化特性及抗氧化活性

  • 杨壮 ,
  • 刘怡琳 ,
  • 李隆熙 ,
  • 刘菡 ,
  • 刘旭 ,
  • 马艳莉 ,
  • 王颉
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  • 1(河北农业大学 食品科技学院,河北 保定,071000)
    2(南阳理工学院 张仲景国医国药学院,河南 南阳,473000)
第一作者:硕士研究生(马艳莉教授和王颉教授为共同通信作者,E-mail:myl_dz@sina.com;wangjie591010@163.com)

收稿日期: 2023-04-09

  修回日期: 2023-05-15

  网络出版日期: 2024-05-09

基金资助

河南省高等学校青年骨干教师培养计划项目(2020GGJS226);南阳市协同创新重大专项(21XTCX12005)

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Process optimization, physicochemical properties, and antioxidant activity of Polygonatum sibiricum polysaccharide prepared by solid-state fermentation

  • YANG Zhuang ,
  • LIU Yilin ,
  • LI Longxi ,
  • LIU Han ,
  • LIU Xu ,
  • MA Yanli ,
  • WANG Jie
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  • 1(College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)
    2(Zhang Zhongjing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473000, China)

Received date: 2023-04-09

  Revised date: 2023-05-15

  Online published: 2024-05-09

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摘要

选取7种乳杆菌进行固态发酵,以黄精多糖得率为评价指标确定最佳发酵菌种。借助单因素试验和响应面曲面分析法对固态发酵黄精工艺进行优化,并分析固态发酵黄精多糖(solid state fermentation Polygonatum sibiricum polysaccharide, SF-PSP)与相同条件下未经发酵黄精多糖(P. sibiricum polysaccharide, PSP)的单糖组成、形态特征、热失重特性以及体外抗氧化活性。结果表明:副干酪乳杆菌ATCC 334为最优发酵菌种,SF-PSP的最佳制备工艺条件为:液料比0.98∶1(mL∶g)、接菌量4.80%、发酵时间51.30 h,此条件下SF-PSP得率为(11.14±0.89)%。SF-PSP主要由甘露糖、半乳糖醛酸、半乳糖和葡萄糖组成,与PSP相比,鼠李糖、半乳糖醛酸、半乳糖含量均提高了4倍。扫描电镜结果表明,与PSP相比,SF-PSP结构更加疏松且有更多致密的小孔。热重分析结果表明2种多糖失重温度节点不同,且SF-PSP保水能力优于PSP。体外抗氧化活性结果表明SF-PSP的DPPH自由基清除率、ABTS阳离子自由基清除率、亚铁离子螯合能力及总抗氧化性均显著高于PSP(P<0.05)。综上,采用SF-PSP可以显著提高黄精多糖的得率和抗氧化活性。

关键词： 菌种筛选; 固态发酵; 黄精多糖; 理化特性; 抗氧化活性

本文引用格式

杨壮 , 刘怡琳 , 李隆熙 , 刘菡 , 刘旭 , 马艳莉 , 王颉 . 固态发酵制备黄精多糖的工艺优化、理化特性及抗氧化活性[J]. 食品与发酵工业, 2024 , 50(7) : 92 -98 . DOI: 10.13995/j.cnki.11-1802/ts.035769

Abstract

Seven kinds of Lactobacillus strains were selected for solid-state fermentation, determined the best fermentation strain based on the yield of Polygonatum sibiricum polysaccharide as the evaluation index.The process of solid-state fermentation P. sibiricum was optimized through single factor test and response surface analysis.The monosaccharide composition, morphology characteristics, thermogravimetric characteristics, and antioxidant activity in vitro of solid-state fermentation P. sibiricum polysaccharide (SF-PSP) and unfermented P. sibiricum polysaccharide (PSP) under the same conditions were analyzed.The results showed that Lactobacillus parasitum ATCC 334 was the best fermentation strain.The optimal preparation process conditions for SF-PSP were as follows:Liquid to material ratio of 0.98∶1 (mL∶g), inoculation rat of 4.80%, and fermentation time of 51.30 h.Under this condition, the yield of the SF-PSP was (11.14±0.89)%.The SF-PSP was mainly composed of mannose, galacturonic acid, galactose and glucose.Compared with PSP, the contents of rhamnose, galacturonic acid and galactose increased by 4 times.The results of scanning electron microscope indicated that the structure of SF-PSP was looser and had more dense pores than PSP.The results of thermogravimetric analysis showed that the weight loss temperature nodes of the two crude polysaccharides were different, the water retention ability of SF-PSP was better than that of PSP.The results of antioxidant activity in vitro showed that DPPH radical scavenging rate, ABTS cationic radical scavenging rate, ferrous ion chelating ability, and total antioxidant activity of SF-PSP were significantly higher than those of PSP(P<0.05).In summary, the use of SF-PSP can significantly improve the yield and antioxidant activity of PSP.

Key words： strain screening; solid state fermentation; Polygonatum sibiricum polysaccharides; physicochemical properties; antioxidant activity

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