该研究从新疆奶疙瘩中分离鉴定一株发酵粘液乳杆菌(Limosilactobacillus fermentum)WHH2438,以商业菌株鼠李糖乳酪杆菌(Lacticaseibacillus rhamnosus)GG为对照,从抗氧化特性、改善肠道屏障功能、耐受性和黏附性等方面,进行体外功效评价。之后以C57BL/6 J小鼠为实验对象,使用液体酒精饲料建立慢性酒精性肝损伤模型,验证菌株的缓解酒精性肝损伤功能。结果显示,该发酵粘液乳杆菌清除DPPH自由基和羟自由基能力显著优于LGG,在保护肠道细胞和肠道屏障方面也有优秀的能力。通过酒精性肝损伤功能动物剂量效价实验,发现菌株WHH2438高剂量组和灭活组的血清谷丙转氨酶和谷草转氨酶相比模型组明显下降、肝脏甘油三脂和还原型谷胱甘肽下降且接近对照,总体有较明显的改善效果,说明该菌株降低酒精对肝脏的氧化损伤,延缓酒精性脂肪肝的形成,具有良好的缓解酒精性肝损伤功能。综上,发酵粘液乳杆菌WHH2438具有良好的缓解慢性酒精性肝损伤功能,且菌株特性优良,能顺利到达肠道,并黏附于肠道上皮细胞,发挥益生功效。
In this study, a strain of Limosilactobacillus fermentum WHH2438 was isolated and identified from Xinjiang yogurt.The antioxidant properties, intestinal barrier function, tolerance and adhesion of the WHH2438 were evaluated in vitro, using the Lacticaseibacillus rhamnosus GG as a control.Subsequently, C57BL/6 J mice were used as experimental subjects, and a chronic alcohol-induced liver injury model was established using liquid alcohol feed to validate the strain's ability to alleviate alcohol-induced liver damage.Results showed that the ability of Limosilactobacillus fermentum to eliminate DPPH and Hydroxyl radical was significantly better than that of LGG, and it also had excellent ability to protect intestinal cells and intestinal barrier.Through the dosage potency test, the high-dose group and inactivated group of the WHH2438 strain showed a significant decrease in serum glutamic-pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase(GOT) compared to the model group.Additionally, liver triglycerides and reduced glutathione (GSH) decreased and approached the control group, indicating an overall significant improvement.This suggested that the strain reduced alcohol-induced oxidative damage to the liver, delayed the formation of alcoholic fatty liver, and possessed a good ability to alleviate alcohol-induced liver injury.In conclusion, Limosilactobacillus fermentum WHH2438 has a good ability of alleviating chronic alcohol-induced liver injury, and it exhibits favorable strain characteristics, successfully reaches the intestine, adheres to intestinal epithelial cells, and exerts probiotic effects.
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