肥胖是一种普遍存在的疾病,与许多严重的并发症有关。为了探究香榧油对肥胖小鼠脂质代谢影响的潜在分子机制,以高脂饮食建立肥胖小鼠模型,评估香榧油的降脂、抗氧化和抗炎症功能。结果表明,与高脂饮食(high-fat diet,HFD)组小鼠相比,香榧油高剂量(Torreya seed oil high-dose,SCH)组小鼠总胆固醇、甘油三酯和低密度脂蛋白胆固醇的水平均显著降低,高密度脂蛋白胆固醇的水平显著升高(P<0.05)。香榧油能显著抑制体重增长,降低肝脏系数和肾周脂肪积累。此外,香榧油干预后能降低肥胖小鼠血清中肿瘤坏死因子-α和白介素6水平,提高超氧化物歧化酶和谷胱甘肽过氧化酶酶活力,改善氧化应激和炎症反应。实时荧光定量PCR(real-time quantitative PCR,RT-PCR)结果显示,与HFD组相比,香榧油低剂量(Torreya seed oil low-dose,SCL)组小鼠固醇调节元件结合蛋白-1c(sterol regulatory element-binding protein-1c, SPEBP-1c)表达水平显著降低(P<0.05),而腺苷酸活化蛋白激酶α(adenosine monophosphate (AMP)-activated protein kinase alpha,AMPKα)和脂肪酸合酶(fatty acid synthase, FAS)的表达水平略有降低但不显著;SCH组小鼠AMPKα、SPEBP-1c和FAS表达均显著降低(P<0.05)。香榧油改善肥胖小鼠脂质代谢紊乱的作用机制可能是通过调节AMPKα/SREBP-1c信号通路,提高抗氧化水平和降低炎症反应实现。
蒋起宏
,
姚诗炜
,
卢红伶
,
蒋陈凯
,
胡文君
,
冯永才
,
陈振滨
,
沈国新
,
相兴伟
,
陈琳
. 香榧油对肥胖小鼠脂质代谢紊乱的作用机制研究[J]. 食品与发酵工业, 2024
, 50(8)
: 176
-181
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DOI: 10.13995/j.cnki.11-1802/ts.035084
Obesity is a widespread disease associated with many serious complications.This study aimed to investigate the potential molecular mechanisms of Torreya seed oil on lipid metabolism in obese mice, an obese mice model established with a high-fat diet to evaluate the hypolipidemic, antioxidant, and anti-inflammatory functions.The levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol were significantly decreased, but the level of high-density lipoprotein cholesterol was significantly increased in the Torreya seed oil high-dose (SCH) group mice compared with the high-fat diet (HFD) group mice (P<0.05).Meanwhile, Torreya seed oil could significantly reduce weight gain and decrease liver and perirenal fat accumulation.Torreya seed oil also reduced the expression levels of tumor necrosis factor-α and interleukin-6, increased the activities of superoxide dismutase and glutathione peroxidase, and improved oxidative stress and inflammatory response.Real-time quantitative PCR (RT-PCR) showed that the expression level of SPEBP-1c in the Torreya seed oil low-dose group was significantly reduced (P<0.05), while the expression levels of adenosine monophosphate (AMP)-activated protein kinase alpha (AMPKα) and fatty acid synthase (FAS) were only slightly reduced.The expression levels of AMPKα, sterol regulatory element-binding protein-1c (SPEBP-1c), and FAS in the SCH group were significantly decreased compared with those of the untreated obese mice (P<0.05).These results suggested that Torreya seed oil improved lipid metabolism disorders by regulating the AMPKα/SREBP-1c signalling pathway expression, increasing antioxidant levels, and reducing inflammatory responses in obese mice.
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