肉类营养物质丰富,营养价值高,但在贮藏和销售中易受不良环境影响发生腐败变质,因此肉类的新鲜度实时监测在其流通过程中至关重要。纳米纤维具有较高的比表面积和较高的孔隙率,多孔的纳米纤维其比表面积和孔隙率会更高,有利于增加传感的灵敏度。实验以聚乳酸为基材,蓝莓花青素为指示材料,使用静电纺丝技术制备了多孔纳米纤维颜色指示膜。扫描电子显微镜表明指示膜具有均匀、光滑的纤维结构,纤维表面具有大小一致的孔隙,有利于花青素的负载以及纳米纤维的快速响应。通过氨响应性探究出指示膜对不同浓度的氨溶液有颜色响应。将指示膜应用于羊肉的新鲜度检测中。结果表明,多孔纳米纤维颜色指示膜在羊肉从新鲜到腐败的过程中,颜色从紫红色变为深灰色,能够被肉眼识别。综上所述,该多孔颜色指示膜能够实现羊肉新鲜度的无损检测,对食品安全检测技术的研究具有借鉴意义。
Meat is rich in nutrients and high in nutritional value but is susceptible to spoilage in storage and distribution due to adverse environmental influences.Therefore, real-time monitoring of meat freshness is vital in its distribution.Nanofibers have higher specific surface area and higher porosity.Porous nanofibers have higher specific surface area and porosity, which is conducive to increasing the sensitivity of sensing.The porous nanofiber color indicator membrane was prepared using the electrostatic spinning technique with degradable polylactic acid as the base material and natural pigment blueberry anthocyanin as the indicator material.The addition of NaHCO3 to the poly-L-lactic acid solution, prepared at 50%-60% humidity can result in fibers with more pores.Scanning electron microscopy showed that the porous color indicator membrane had a uniform and smooth fiber structure with uniformly sized pores on the fiber surface, which facilitated the loading of anthocyanins and the rapid response of the nanofibers.The porous color indicator membrane was probed for its color response to different concentrations of ammonia solution by ammonia responsiveness.The porous color indicator membrane was applied to mutton stored at 25 ℃ to determine the changes of TVB-N and the total color difference of the porous color indicator membrane.Results showed that the color and ΔE of the porous color indicator membrane changed significantly under 25 ℃ storage.The porous nanofiber color indicator membrane could be identified by the naked eye as the color of mutton changes from purplish red to dark gray during the process from fresh to spoiled.In summary, this porous color indicator membrane enables nondestructive detection of mutton freshness and has implications for the study of food safety detection technology.
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