该研究是以提高麦醇溶蛋白(gliadin, Gli)纳米颗粒(gliadin nanoparticles, GNPs)稳定性为目标,利用银耳多糖(Tremella fuciformis polysaccharides, TFPs)的乳化性能,探究TFPs添加量对GNPs稳定性的影响。采用反溶剂法制备银耳多糖和麦醇溶蛋白复合载体颗粒(gliadin-Tremella fuciformis polysaccharides composite nanoparticles, G/T NPs),并利用纳米粒度仪、傅里叶变换红外光谱仪、热场发射扫描电镜对G/T NPs的粒径和电位、相互作用、微观结构进行分析。结果表明,TFPs和Gli之间通过静电作用力和氢键作用力相结合,形成GNPs在内、TFPs外层附着的复合颗粒;TFPs的添加能够有效提高GNPs体系的稳定性,减少颗粒之间的聚集,制得的G/T4∶1 NPs的平均粒径、多分散指数(polydispersion index, PDI)、ζ-电位分别为(198.5±2.6) nm、0.269±0.005、(36.7±0.6) mV,且具有pH响应性。该研究为后续构建疏水营养成分的包埋和运载体系奠定了基础。
To improve the stability of gliadin (Gli) nanoparticles (GNPs), the emulsification property of Tremella fuciformis polysaccharides (TFPs) was used to explore the influence of the content of TFPs on the stability of GNPs.The composite nanoparticles of TFPs and Gli (G/T NPs) were prepared by the anti-solvent method.The Z-average, ζ-potential, interaction, and microstructure of the composite carrier particles were analyzed by Zetasizer, Fourier transform infrared spectrometer, and thermal field emission scanning electron microscopy.Results showed G/T NPs were formed by the combination of the electrostatic force and the hydrogen bond force, with the inner GNPs and TFPs wrapped in the outer layer.TFPs could effectively improve the stability of the GNPs system and reduce the aggregation of particles during rotary evaporation.The Z-average, polydispersion index (PDI), and ζ-potential of G/T4∶1 NPs were (198.5±2.6) nm, (0.269±0.005), (36.7±0.6) mV, respectively, and showed pH responsiveness.This study laid the foundation for the subsequent construction of the encapsulation and delivery system of hydrophobic nutrients.
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