The proper sealing of the pickle jar/pond is a crucial factor that impacts the blooming of pickles.To elucidate the impact of air on yeast flowering, transcriptomics and bioinformatics were employed to scrutinize and unravel pivotal genes that modulate yeast florescence.A total of 183 differentially expressed genes were identified in the flower-producing yeast cultured with or without air, including 42 up-regulated genes and 141 down-regulated genes (P<0.05).The differentially expressed genes were predominantly enriched in glucosidase activity, arabinose dehydrogenase activity, and ribosomal subunit.The differentially expressed genes were found to be enriched in 34 pathways.Among them, the ribosome pathway was identified as the most significant one, with 19 up-regulated genes such as RPL4, RPL10 and RPSA, and 2 down-regulated ones including RPS2 and RPS15.In addition, FAD2 was up-regulated in both fatty acid synthesis pathway and unsaturated fatty acid synthesis pathway while 6 genes (FAS1, FAS2 and SCD etc.) were down-regulated.The qRT-PCR results demonstrated that the expression levels of pivotal genes, namely FAD2, RPL4 and RPL10, were significantly up-regulated in air culture (P<0.05), which was consistent with the transcriptome-based predictions.The findings establish a theoretical foundation for understanding the flowering mechanism of yeast in pickles and optimizing pickle quality control.
[1] 邓维琴, 伍亚龙, 陈功, 等.多代发酵泡菜母水微生物多样性及其理化指标动态研究[J].食品与发酵工业, 2018, 44(8):49-56.
DENG W Q, WU Y L, CHEN G, et al.Study on microbial diversity and physiochemical index of multi-generational fermented Paocai brine[J].Food and Fermentation Industries, 2018, 44(8):49-56.
[2] 黄润秋, 唐垚, 费敏, 等.四川泡菜盐渍液处理及应用的研究进展[J].食品与发酵工业, 2023, 49(1):330-336.
HUANG R Q, TANG Y, FEI M, et al.Research progress on treatment and application of Sichuan Paocai saline[J].Food and Fermentation Industries, 2023, 49(1):330-336.
[3] LIU L, SHE X, QIAN Y, et al.Effect of different fermenting containers on the deterioration of Sichuan pickle[J].LWT, 2019, 111:829-836.
[4] 王猛. 四川泡菜腐败过程中腐败细菌的分离鉴定及其腐败特性分析[D].成都:西华大学, 2016.
WANG M.Isolation, identification and characteristics analysis of spoilage bacteria in Sichuan pickle during the deteriorating process[D].Chengdu:Xihua University, 2016.
[5] RAO Y, QIAN Y, SHE X, et al.Pellicle formation, microbial succession and lactic acid utilisation during the aerobic deteriorating process of Sichuan pickle[J].International Journal of Food Science & Technology, 2018, 53(3):767-775.
[6] 何鹏晖, 厍晓, 钱杨, 等.发酵蔬菜中腐败微生物及其防控的研究进展[J].食品工业科技, 2017, 38(11):374-378;384.
HE P H, SHE X, QIAN Y, et al.Research advances of spoilage microorganisms and their preventive measures in fermented vegetables[J].Science and Technology of Food Industry, 2017, 38(11):374-378;384.
[7] LU J Y, HU X C, REN L J.Biofilm control strategies in food industry:Inhibition and utilization[J].Trends in Food Science & Technology, 2022, 123:103-113.
[8] 黄道梅, 胡露, 贾秋思, 等.多菌协同发酵萝卜过程中不同盐浓度对菌相的影响[J].食品与发酵工业, 2016, 42(6):36-43.
HUANG D M, HU L, JIA Q S, et al.Effect of different salinity on the microbial community diversity during strains synergism fermentation of pickled radish[J].Food and Fermentation Industries, 2016, 42(6):36-43.
[9] YANG Y, LIAN Y Y, YIN S M, et al.Inhibition of Lactobacillus fermentum SHY10 on the white membrane production of soaked pickled radish[J].Food Science & Nutrition, 2022, 10(7):2236-2244.
[10] FENG G R, LI X D, WANG W J, et al.Effects of peptide thanatin on the growth and transcriptome of Penicillium digitatum[J].Frontiers in Microbiology, 2020, 11:606482.
[11] 文明明, 毕洁, 贺艳萍, 等.高糖饮食抑制后代雄性果蝇寿命和育性及其作用机制[J].现代食品科技, 2022, 38(10):9-18.
WEN M M, BI J, HE Y P, et al.Lifespan and fertility of male Drosophila melanogaster decreased by A high-sucrose diet and the related mechanisms[J].Modern Food Science and Technology, 2022, 38(10):9-18.
[12] 李姝, 童钰琴, 杨舒淋, 等.β-烟酰胺单核苷酸促秀丽隐杆线虫生长及其作用机制[J].食品工业科技, 2023, 44(19):417-426.
LI S, TONG Y Q, YANG S L, et al.Effect of β-nicotinamide mononucleotide on the growth promotion of Caenorhabditis elegans and its mechanism[J].Science and Technology of Food Industry, 2023, 44(19):417-426.
[13] 刘永泉. 基于转录组学和代谢组学研究脐橙-黄龙病菌-柑橘木虱的互作机制[D].南昌:南昌大学, 2022.
LIU Y Q.The interaction mechanism of navel orange-candidatus liberibacter asiaticus-asian citrus psyllids based on transcriptomics and metabolomics[D].Nanchang:Nanchang University, 2022.
[14] 杨恺清, 赵景霖, 毛小琴.核糖体蛋白的核糖体外功能及其在疾病中的作用[J].生理科学进展, 2021, 52(3):227-230.
YANG K Q, ZHAO J L, MAO X Q.The extra-ribosomal functions of ribosomal proteins and their role in disease[J].Progress in Physiological Sciences, 2021, 52(3):227-230.
[15] 郭文环. 间充质干细胞对阿尔茨海默症模型小鼠的治疗作用及相关机制研究[D].北京:北京科技大学, 2022.
GUO W H.Therapeutic effects and mechanisms of mesenchymal stem cell on Alzheimer’s disease model mice[D].Beijing:University of Science and Technology Beijing, 2022.
[16] VILAS-BOAS F, BAGULHO A, JERÓNIMO A, et al.Data on intracellular localization of RPSA upon alteration of its redox state[J].Data in Brief, 2016, 6:311-315.
[17] 任红玉. 禽呼肠孤病毒σA蛋白与宿主蛋白HSP70、HSP40和RPL4相互作用的初步研究[D].南宁:广西大学, 2019.
REN H Y.Preliminary study on the interaction between avian reovirus σA protein and host proteins HSP70, HSP40 and RPL4[D].Nanning:Guangxi University, 2019.
[18] RAMU V S, DAWANE A, LEE S, et al.Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance[J].Molecular Plant Pathology, 2020, 21(11):1481-1494.
[19] 魏荷芬, 施明雨, 郭亭, 等.耐盐异养好氧氨氧化菌株的筛选、鉴定及特性研究[J].生物加工过程, 2023, 21(1):76-82.
WEI H F, SHI M Y, GUO T, et al.Screening, identification and characterization of salt-tolerant heterotrophic aerobicammoxidation strain[J].Chinese Journal of Bioprocess Engineering, 2023, 21(1):76-82.
[20] 魏小艳, 李勇, 谭宇萍, 等.薏苡delta-12脂肪酸去饱和酶基因FAD2的克隆和表达分析[J].药学学报, 2021, 56(5):1478-1485.
WEI X Y, LI Y, TAN Y P, et al.Cloning and expression analysis of delta-12 fatty acid desaturase gene in Coix lacryma-jobi L[J].Acta Pharmaceutica Sinica, 2021, 56(5):1478-1485.
[21] 王霞, 林婉玲, 李来好, 等.高效液相色谱-蒸发光散射法分析海鲈肌肉磷脂组成[J].中国油脂, 2020, 45(9):97-101.
WANG X, LIN W L, LI L H, et al.Determination of phospholipids composition in Lateolabrax japonicus muscle by HPLC-ELSD[J].China Oils and Fats, 2020, 45(9):97-101.
[22] 余鹏, 郑方盈, 余义和, 等.采前喷施胺鲜酯对葡萄果实贮藏期间膜脂代谢的影响[J].食品科学, 2023,44(23):159-164.
YU P, ZHENG F Y, YU Y H, et al.Effects of preharvest spraying of diethyl aminoethyl hexanoate on membrane lipid metabolism of postharvest grapes during storage[J].Food Science, 2023,44(23):159-164.
