Red rice is the largest yield of colored rice in China, with biological activities such as antioxidant and anti-inflammatory.But its water-soluble polysaccharide resources have not been exploited.Non-starch polysaccharides from red rice bran were prepared from red rice bran as raw material, by extracting with hot water, decoloring, deproteinizing, alcohol precipitation, and dialysis.Different fractions of extractive polysaccharides were isolated and purified using DEAE-52 Cellulose and Sephadex G-200, with the largest proportion being the RRBP4 component.Molecular weight, monosaccharide composition, main and side chains, glycosidic bonds, branching degree, and triple helix conformation of RRBP4 were identified by using HPLC, FT-IR, complete acid hydrolysis, partial acid hydrolysis, β-elimination, I2-KI experiment, and Congo red test methods.Result showed that the relative molecular weight of RRBP4 was 1.65×105 Da.RRBP4 was composed of mannose, ribose, rhamnose, galacturonic acid, glucuronic acid, glucose, galactose, xylose, and arabinose, the mole ratio of them was 0.29∶1.93∶0.35∶1.41∶0.50∶1.00∶5.58∶1.67∶2.56.RRBP4 was an acidic heteroglycan dominated by galactose.There were 62.40% polysaccharides located in the side chain, which was a highly branched polysaccharide.RRBP4 was a polysaccharide with α-glycosidic bonds and amino acids and had a triple helix structure.The structural analysis of water-soluble non-starch polysaccharides from red rice bran provided a scientific essential data for the development of their application in the food industry.
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