研究报告

新疆昌吉奶疙瘩中乳酸菌的分离鉴定及发酵杏酱性能分析

  • 达菊庆 ,
  • 武运 ,
  • 吴聪 ,
  • 罗晓红 ,
  • 马文瑞 ,
  • 唐弦 ,
  • 汤凯 ,
  • 杜木英
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  • 1(新疆农业大学 食品科学与药学学院,新疆 乌鲁木齐,830052)
    2(新疆天润生物科技股份有限公司,新疆 乌鲁木齐,830088)
    3(西南大学 食品科学学院,重庆,400715)
第一作者:硕士研究生(武运教授为通信作者,E-mail:wuyunster@sina.com)

收稿日期: 2023-07-24

  修回日期: 2023-08-24

  网络出版日期: 2024-07-11

基金资助

科技部国家重点研发计划项目(2022YFE0120800)

Isolation and identification of lactic acid bacteria from cheese in Changji, Xinjiang and analysis of their fermentation performance in apricot jam

  • DA Juqing ,
  • WU Yun ,
  • WU Cong ,
  • LUO Xiaohong ,
  • MA Wenrui ,
  • TANG Xian ,
  • TANG Kai ,
  • DU Muying
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  • 1(College of Food Science and Pharmacy, Xinjiang Agricultural University, Urmuqi 830052, China)
    2(Xinjiang Tianrun Biotechnology Co.Ltd., Urumqi 830088, China)
    3(College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2023-07-24

  Revised date: 2023-08-24

  Online published: 2024-07-11

摘要

为探究奶疙瘩源乳酸菌发酵杏酱性能,从新疆昌吉采收15份传统手工奶疙瘩样品,采用传统平板划线法、生理生化分析、16S rDNA基因序列测序鉴定从中筛选出的7株菌,并对其安全性、耐高可溶性固形物、耐高酸及发酵杏酱过程中活菌数、总酸、及发酵前后基本理化指标分析。经鉴定,其中菌株YP01为罗伊氏乳杆菌(Lactobacillus reuteri)、菌株YP04、YP09、YP17和YP19为魏斯氏菌(Weissella confusa)、菌株YP11和YP12为植物乳杆菌(Lactiplantibacillus plantarum)。菌株YP01、YP04、YP09、YP17生长活性较高、安全性较强,菌株YP01、YP04耐受性强。测定以上4株菌发酵杏酱过程中活菌数、总酸及发酵前后理化指标与感官品质,结果显示罗伊氏乳杆菌(Lactobacillus reuteri)YP01更适合杏酱发酵,魏斯氏菌(Weissella confusa)YP04次之。2株菌均具有良好的碳源利用能力。罗伊氏乳杆菌(Lactobacillus reuteri)YP01与魏斯氏菌(Weissella confusa)YP04具有发酵杏酱潜力,可作为发酵杏酱待选菌株。

本文引用格式

达菊庆 , 武运 , 吴聪 , 罗晓红 , 马文瑞 , 唐弦 , 汤凯 , 杜木英 . 新疆昌吉奶疙瘩中乳酸菌的分离鉴定及发酵杏酱性能分析[J]. 食品与发酵工业, 2024 , 50(12) : 84 -91 . DOI: 10.13995/j.cnki.11-1802/ts.036880

Abstract

15 traditional handmade cheese from Changji, Xinjiang were collected to investigate the performance of cheese-derived lactic acid bacteria in fermenting apricot jam.Finally, seven strains were selected by using traditional plate marking, physiological and biochemical analysis, and 16S rDNA gene sequencing methods.The strain’s safety, high soluble solids resistance, high acid resistance, and some indicators, including number of viable bacteria, total acid, and basic physicochemical indicators were analyzed before and after the fermentation.After identification, the strain YP01 belongs to Lactobacillus reuteri. Strains YP04, YP09, YP17, and YP19 belong to Weissella confusa.Strains YP11 and YP12 belong to Lactiplantibacillus plantarum.Among them, strains YP01, YP04, YP09, and YP17 have high growth activity and strong safety, while strains YP01 and YP04 have strong tolerance.The number of viable bacteria, total acids, and physiological-biochemical indicators before and after fermentation, as well as sensory quality, were measured during the fermentation of apricot jam using the above four lactic acid bacteria.The results showed that Lactobacillus reuteri YP01 was more suitable for apricot jam fermentation, followed by Weissella confusa YP04.Both of them had strong carbon source utilization capacity.Lactobacillus reuteri YP01 and Weissella confusa YP04 had the potential to ferment apricot jam, which could be used as potential fermenting strains for apricot jam fermentation.

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