食品与发酵工业 >

2024 , Vol. 50 >Issue 11: 18 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037968

研究报告

一株猪源德尔卑沙门氏菌的分离鉴定、生物学特性以及全基因组序列分析

  • 蔡秋慧 ,
  • 龚莹婷 ,
  • 李港回 ,
  • 彭钢 ,
  • 孟静南 ,
  • 王俊颖 ,
  • 翟立公
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  • (安徽科技学院 食品工程学院,安徽 滁州,233100)
第一作者:硕士研究生(翟立公副教授为通信作者,E-mail:gavin340@126.com)

收稿日期: 2023-11-16

  修回日期: 2024-02-07

  网络出版日期: 2024-07-12

基金资助

安徽省自然科学基金项目(2008085MC89);安徽省高校自然学科重大项目(2023AH040277)

收起

Isolation, biological characterization, and whole genome sequence analysis of a porcine strain of Salmonella Derby

  • CAI Qiuhui ,
  • GONG Yingting ,
  • LI Ganghui ,
  • PENG Gang ,
  • MENG Jingnan ,
  • WANG Junying ,
  • ZHAI Ligong
Expand
  • (School of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China)

Received date: 2023-11-16

  Revised date: 2024-02-07

  Online published: 2024-07-12

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摘要

沙门氏菌作为人畜共患的致病菌,更是极易产生耐药性,严重影响了人类与动物的健康。为了更好的了解动物源性沙门氏菌的生物学和分子特性。对安徽凤阳县某市场猪肉样本进行细菌分离纯化、血清型鉴定、药敏实验和毒力基因检测,同时通过全基因组测序结果进行毒力、耐药基因注释等分子生物学信息分析,并通过qPCR对毒力基因进行验证。分离鉴定出1株德尔卑沙门氏菌,分子分型为ST1498型,将其命名为G-1B,该菌株对环丙沙星、卡那霉素和复方新诺明敏感。全基因组长度为4 805 494 bp,预测出4 660个基因,其中包含559个毒力基因与37个耐药基因。该研究为了解猪源德尔卑沙门氏菌的遗传背景和生物学特性奠定了基础,为进一步探讨德尔卑沙门氏菌致病性、耐药机制和分子进化规律提供参考依据。

关键词： 德尔卑沙门氏菌; 环境胁迫; 全基因组测序; 基因功能注释; 生物信息学分析

本文引用格式

蔡秋慧 , 龚莹婷 , 李港回 , 彭钢 , 孟静南 , 王俊颖 , 翟立公 . 一株猪源德尔卑沙门氏菌的分离鉴定、生物学特性以及全基因组序列分析[J]. 食品与发酵工业, 2024 , 50(11) : 18 -26 . DOI: 10.13995/j.cnki.11-1802/ts.037968

Abstract

As a zoonotic food-borne pathogen, Salmonella spp.is particularly susceptible to drug resistance, which has a serious impact on the health of both humans and animals.This study aimed to gain a better understanding of the biology and molecular characterization of Salmonella spp.of animal origin.Bacterial isolation and purification, serotype identification, 16S rRNA gene identification, drug susceptibility testing, and virulence gene detection have been carried out on samples of pig meat from a market in Fengyang county, Anhui province, China, while molecular biological information, such as virulence and resistance gene annotation, was analyzed by whole-genome sequencing results and virulence genes verified by qPCR.A strain of Salmonella Derby ST1498 was isolated and identified as G-1B.It was susceptible to ciprofloxacin, kanamycin, and cotrimoxazole.The total genome length was 4 805 494 bp and 4 660 genes were predicted, including 559 virulence genes with 37 resistance genes.This study lays the foundation for the understanding of the genetic background and biological characteristics of Salmonella Derby of pig origin, provides a reference basis for the further investigation of the pathogenicity, drug resistance mechanism and molecular evolution of Salmonella Derby, and is of great significance for the subsequent studies of strain G-1B.

Key words： Salmonella Derby; environmental stress; whole-genome sequencing; gene function annotation; bioinformatics analysis

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